Mechanical Stimulation of Cells Through Scaffold Design for Tissue Engineering

Oliver-Urrutia, Carolina; García, Ma. Victoria Domínguez; Estrada, Jaime Flores; camacho, antonio laguna; Cadena, Julieta Castillo; Merino, Miriam Veronica Flores

doi:10.5772/intechopen.69925

Cited by 3 publications

(2 citation statements)

References 55 publications

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“…Second, it helps with the fusion, alignment, and maturation of the myotube. Third, it helps with the proliferation and differentiation of muscle cells [145], muscle alignment [146] and muscle growth and maturation [147,148]. There are various methods of mechanical stimulation of cells [149].…”

Section: Mechanical Stimulationmentioning

confidence: 99%

Consumer Acceptance and Production of In Vitro Meat: A Review

et al. 2022

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In vitro meat (IVM) is a recent development in the production of sustainable food. The consumer perception of IVM has a strong impact on the commercial success of IVM. Hence this review examines existing studies related to consumer concerns, acceptance and uncertainty of IVM. This will help create better marketing strategies for IVM-producing companies in the future. In addition, IVM production is described in terms of the types of cells and culture conditions employed. The applications of self-organising, scaffolding, and 3D printing techniques to produce IVM are also discussed. As the conditions for IVM production are controlled and can be manipulated, it will be feasible to produce a chemically safe and disease-free meat with improved consumer acceptance on a sustainable basis.

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Section: Mechanical Stimulationmentioning

confidence: 99%

Consumer Acceptance and Production of In Vitro Meat: A Review

et al. 2022

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“…Mechanical stimulation is a very promising strategy, based on which novel therapeutic biomedical technologies can be developed. Mechanical stimulation is known to affect the growth and differentiation of stem cells [1], and thus, various applications employing this strategy have been proposed in the fields of tissue engineering and regenerative medicine [2]. Interestingly, mechanoresponsive genes have been identified, highlighting that mechanical stress alone can regulate the transcription of certain genes [3].…”

Section: Introductionmentioning

confidence: 99%

Magnetomechanical Stress-Induced Colon Cancer Cell Growth Inhibition

Spyridopoulou

Aindelis

Sarafidis

et al. 2022

JNT

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The application of magnetomechanical stress in cells using internalized magnetic nanoparticles (MNPs) actuated by low-frequency magnetic fields has been attracting considerable interest in the field of cancer research. Recent developments prove that magnetomechanical stress can inhibit cancer cells’ growth. However, the MNPs’ type and the magnetic field’s characteristics are crucial parameters. Their variability allows multiple combinations, which induce specific biological effects. We previously reported the antiproliferative effects induced in HT29 colon cancer cells by static-magnetic-field (200 mT)-actuated spherical MNPs (100 nm). Herein, we show that similar growth inhibitory effects are induced in other colon cancer cell lines. The effect of magnetomechanical stress was also examined in the growth rate of tumor spheroids. Moreover, we examined the biological mechanisms involved in the observed cell growth inhibition. Under the experimental conditions employed, no cell death was detected by PI (propidium iodide) staining analysis. Flow cytometry and Western blotting revealed that G2/M cell cycle arrest might mediate the antiproliferative effects. Furthermore, MNPs were found to locate in the lysosomes, and a decreased number of lysosomes was detected in cells that had undergone magnetomechanical stress, implying that the mechanical activation of the internalized MNPs could induce lysosome membrane disruption. Of note, the lysosomal acidic conditions were proven to affect the MNPs’ magnetic properties, evidenced by vibrating sample magnetometry (VSM) analysis. Further research on the combination of the described magnetomechanical stress with lysosome-targeting chemotherapeutic drugs could lay the groundwork for the development of novel anticancer combination treatment schemes.

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