Computational Framework to Evaluate the Hydrodynamics of Cell Scaffold Geometries

Puleri, Daniel F.; Roychowdhury, Sayan; Ames, Jeff; Randles, Amanda

doi:10.1109/embc44109.2020.9176313

Cited by 1 publication

(1 citation statement)

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“…4 Cell scaffolds can support cell growth and migration, determine cell phenotype, and maintain structural integrity. 5 Hepatocytes can be cultured on scaffolds made from various substrates, such as natural biopolymers and synthetic biopolymers. 6 The most commonly used natural biopolymers include silk, collagen, chitosan, hyaluronan, gelatin and alginate.…”

Section: Introductionmentioning

confidence: 99%

Optimization of decellularized liver matrix-modified chitosan fibrous scaffold for C3A hepatocyte culture

Zhou

Zhao

2022

J Biomater Appl

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Hepatocyte scaffold is an essential part in bioartificial liver device. We have designed a novel hepatocyte scaffold based on porcine liver extracellular matrix (ECM) and chitosan (CTS) fabrics. This CTS-ECM scaffold can improve cell adhesion and proliferation. In the present study, an orthogonal test was designed to optimize the CTS/ECM composite scaffold, in which ECM concentration, EDC concentration and EDC to NHS ratio were taken as factors, proportion of nitrogen element and hydroxyproline content as indicators. The cytocompatibility of the novel scaffold for C3A hepatocytes was analyzed in vitro. The orthogonal test demonstrated that the optimal scaffold should be based on ECM concentration of 5 mg/mL, EDC concentration of 5 mg/mL, and EDC to NHS ratio 1:1. C3A hepatocytes cultured on the optimized CTS-ECM scaffolds showed stronger proliferation and functionality than those on Cytodex3 microcarriers ( p < 0.05). The CTS/ECM composite scaffold may be widely used as a promising hepatocyte culture carrier, especially in bioartificial liver support systems.

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Section: Introductionmentioning

confidence: 99%