Combined effect of chemotherapy and meristem culture on sugarcane mosaic virus elimination in sugarcane

Balamuralikrishnan, M.; Doraisamy, Sabitha; Ganapathy, T.; Viswanathan, R.

doi:10.1007/bf02956875

Cited by 15 publications

(13 citation statements)

References 7 publications

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“…The inclusion of 0.1 mg l -1 BA (6-Benzyladenine) and 0.015 mg l -1 KIN (DO4) in the initiation medium resulted in shoot development in 100% meristems and a significantly higher number of shoots/meristem (1,370 ± 10), compared with other routes tested after 12 weeks in culture. This result supports the findings of previous work (Victoria et al 1999;Visessuwan et al 1999;Chatenet et al 2001;Balamuralikrishnan et al 2002;Cha-um et al 2006;Ali et al 2008) where direct organogenesis was used for meristem culture. Further investigations to establish virus elimination were based on meristems cultured using this morphogenic route.…”

Section: Resultssupporting

confidence: 95%

“…In contrast, viral particles in meristems larger than 2 mm were not eradicated by the thermotherapy as plants from these meristems tested positive for both viruses after 0 and 12 months acclimation (Table 4). This observation correlates with previous findings that reported virus-free sugarcane plants via organogenesis from explants sized 0.3-1 mm (Victoria et al 1999;Chatenet et al 2001;Balamuralikrishnan et al 2002;Parmessur et al 2002). Consequently, one way of eliminating viruses in sugarcane plants is to combine thermotherapy and optimal meristem size (B2 mm).…”

Section: Effect Of Meristem Source Size and Thermotherapy On Virus Esupporting

confidence: 93%

“…Consequently, one way of eliminating viruses in sugarcane plants is to combine thermotherapy and optimal meristem size (B2 mm). Other techniques that have reported to inactivate viruses include chemo-and cryotherapy (Balamuralikrishnan et al 2002;Wang et al 2007, respectively).…”

Section: Effect Of Meristem Source Size and Thermotherapy On Virus Ementioning

confidence: 98%

“…Similarly in meristems from node shoots, 53.9% of meristems size A generated shoots while 100% larger meristems developed shoots (Table 3). Category A meristems are sensitive to desiccation, phenolics and damage during dissection, factors that could negatively influence shoot development (Balamuralikrishnan et al 2002). As viruses may be present in meristems larger than 1 mm (Victoria et al 1999;Chatenet et al 2001;Parmessur et al 2002), this work assessed the use of thermotherapy to eradicate viruses from larger meristems.…”

Section: Effect Of Meristem Source Size and Thermotherapy On Virus Ementioning

confidence: 99%

“…As only the meristematic dome and the immediate covering (1st leaf primordia) are usually virus-free (Grout 1990;Kane 2005), the size of the meristem excised is critical. A literature review indicates that for virus elimination in sugarcane the size of the meristem excised should be in the range of 0.2-1.5 mm in length (Visessuwan et al 1991(Visessuwan et al , 1999Victoria et al 1999;Chatenet et al 2001;Fitch et al 2001;Balamuralikrishnan et al 2002;Parmessur et al 2002;Cha-um et al 2006;Zhang et al 2006).…”

Section: Introductionmentioning

confidence: 98%

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Elimination of virus and rapid propagation of disease-free sugarcane (Saccharum spp. cultivar NCo376) using apical meristem culture

Ramgareeb

Snyman

Antwerpen

et al. 2009

Plant Cell Tiss Organ Cult

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The use of apical meristem culture for simultaneous virus elimination and shoot proliferation in sugarcane was assessed. Virus-free plants were propagated from Sugarcane mosaic virus and Sugarcane yellow leaf virus-infected material of the South African commercial cultivar, NCo376. A combination of thermotherapy by hot water treatment of stem sections (nodes) and subsequent germination of vegetative buds at 40°C and optimal meristem size were key factors for the production of virus-free plants. Only meristems of 2 mm in length or of a smaller size (but [0.5 mm) resulted in virus-free sugarcane. Shoot induction and proliferation via direct organogenesis were achieved on Murashige and Skoog nutrient medium supplemented with 0.

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Section: Resultssupporting

confidence: 95%

Section: Effect Of Meristem Source Size and Thermotherapy On Virus Esupporting

confidence: 93%

Section: Effect Of Meristem Source Size and Thermotherapy On Virus Ementioning

confidence: 98%

Section: Effect Of Meristem Source Size and Thermotherapy On Virus Ementioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 3 more Smart Citations

Elimination of virus and rapid propagation of disease-free sugarcane (Saccharum spp. cultivar NCo376) using apical meristem culture

Ramgareeb

Snyman

Antwerpen

et al. 2009

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

Plant Tissue Culture and Genetic Transformation for Crop Improvement

Gosal

Kang

2012

Improving Crop Resistance to Abiotic Stress

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Thermotherapy, Chemotherapy, and Meristem Culture in Banana

Lassois

Lepoivre

Swennen

et al. 2012

Methods in Molecular Biology

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Bananas that provide a staple food to the millions of people are adversely affected by several viruses such as Banana bunchy Top Virus (BBTV), Banana Streak Virus (BSV), and Cucumber Mosaic Virus (CMV). These viruses are known to have a devastating effect on crop production and constraint to the international exchange and conservation of banana germplasm-a cornerstone for breeding new cultivars. The viruses are particularly problematic in vegetative propagated crops, like bananas, because of their transmission in the planting material. Different virus eradication techniques have been developed, such as thermotherapy, chemotherapy, and meristem culture for providing virus-free planting material. Meristem culture proved to be the most effective procedure to eradicate phloem-associated viruses. This method requires isolation of meristematic dome of plant under the aseptic conditions and culture in an appropriate nutrient medium to develop new virus-free plants. Thermotherapy is another widely used virus eradication technique, which is initially carried out on in vivo or in vitro plants and eventually combined with meristem culture technique. The plantlets are initially grown at 28°C day temperature and increase it by 2°C per day until reaches 40°C and the night temperature at 28°C; maintain plants at 40°C for 4 weeks; excise meristem and culture onto the regeneration medium. In chemotherapy technique, antiviral chemical compound Virazole(®) is applied on meristem culture. Combination of these techniques is also applied to improve the eradication rate.

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Combined effect of chemotherapy and meristem culture on sugarcane mosaic virus elimination in sugarcane

Cited by 15 publications

References 7 publications

Elimination of virus and rapid propagation of disease-free sugarcane (Saccharum spp. cultivar NCo376) using apical meristem culture

Elimination of virus and rapid propagation of disease-free sugarcane (Saccharum spp. cultivar NCo376) using apical meristem culture

Plant Tissue Culture and Genetic Transformation for Crop Improvement

Thermotherapy, Chemotherapy, and Meristem Culture in Banana

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