Thermotherapy, Chemotherapy, and Meristem Culture in Banana

Lassois, Ludivine; Lepoivre, Philippe; Swennen, Rony; houwe, Ines Van den; Panis, Bart

doi:10.1007/978-1-62703-074-8_32

Cited by 20 publications

(20 citation statements)

References 9 publications

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“…The accessions that have tested positive for a virus will undergo a sanitation process: thermotherapy and chemotherapy are combined with meristem culture to eliminate the infecting virus (Lassois et al 2013). After therapy, the accession is tested again after 3 and 6 months of greenhouse growth and, if negative, can be available for distribution.…”

Section: Virus Indexingmentioning

confidence: 99%

Safeguarding and using global banana diversity: a holistic approach

houwe¹,

Chase

Sardos

et al. 2020

CABI Agric Biosci

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The CGIAR genebank International Musa Germplasm Transit Centre (ITC) currently holds 1617 banana accessions from 38 countries as an in vitro collection, backed-up by a cryopreserved collection to safeguard global Musa diversity in perpetuity. The ITC also serves as a vital safety backup and transit centre for national banana genebanks and ensures that germplasm is clean of pests and diseases and freely available under the International Treaty on Plant Genetic Resources for Food and Agriculture. In more than 35 years of activity, the ITC has distributed over 18,000 banana accession samples to researchers and farmers in 113 countries. Ex situ conservation of vegetatively-propagated crops such as banana poses very particular challenges. Maintaining the ITC genebank is labor intense and costly. Efficiencies are sought through research and development of techniques on detecting viruses, the genetic integrity of accessions, and on innovative means of safeguarding banana diversity, such as conserving populations of wild species by seed banking. Although the conservation of global banana diversity is the main objective of the ITC, significant value comes from its holistic approach to better understand and promote its germplasm through numerous research activities and resources. Techniques for morphological and molecular characterization serve to identify and describe the collection, while also determining what gaps should be filled by collecting missions with national partners. The evaluation of desirable agronomic traits inherent in Musa spp. are investigated by a high-throughput phenotyping platform, which helps breeding programs to select cultivars resistant or tolerant to biotic and abiotic stresses. Genomic and bioinformatic studies of several banana wild relatives greatly enhance our understanding of Musa genetic diversity, links to important phenotypic traits and bring new methods for management of the collection. Collectively, these research activities produce enormous amounts of data that require curation and dissemination to the public. The two information systems at the ITC, Musa Genebank Management System and the Musa Germplasm Information System, serve to manage the genebank activities and to make public germplasm-related data for over 30 banana collections worldwide, respectively. By implementing the 10-year workplan set out in the Global Strategy for the Conservation and Use of Musa Genetic Resources, the network MusaNet supports Musa researchers and stakeholders, including the ITC, and most importantly, links to the world’s banana-producing countries via three regional banana networks.

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Section: Virus Indexingmentioning

confidence: 99%

Safeguarding and using global banana diversity: a holistic approach

houwe¹,

Chase

Sardos

et al. 2020

CABI Agric Biosci

Self Cite

View full text Add to dashboard Cite

show abstract

“…Other critical points need to be considered; one of the main difficulties with SAM culture of ranunculus is the high mortality caused by endogenous bacterial contamination. Moreover, the excision of a portion of apical meristem small enough to guarantee a healthy plant may lead to a high risk of non‐viability (Lassois et al ., ). In the current study, sterile and viable SAM explants were obtained from 6% of the meristems excised from buds of a virus‐infected Yellow 1 plant.…”

Section: Discussionmentioning

confidence: 97%

RT‐PCR tests for sensitive detection of the major Ranunculus‐infecting viruses: field and in vitro applications

Sacco

Borghi

Rabaglio³

et al. 2018

Plant Pathology

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Buttercup (Ranunculus asiaticus, family Ranunculaceae) hybrids are considered a popular and valuable ornamental crop in Europe and are cultivated both for cut flowers and for pot or border plants. In the Liguria region of northern Italy, Ranunculus hybrid cultivation has dramatically increased over recent years and the related sanitary issues regarding virus infection need to be carefully monitored in order to maintain commercial competitiveness. Presently, ELISA is the most used diagnostic tool for virus diagnosis, but if ‘mother plants’ with high economic value are to be tested, more sensitive molecular diagnostic tools are needed, together with efficient protocols for in vitro culture to eradicate viruses. In this study, new RT‐PCR protocols were tested on virus‐infected Ranunculus hybrid plants, indexed for the 13 most important Ranunculus‐infecting viruses. Rapid, uniform and effective procedures for molecular diagnosis were devised in order to promote an easy technology transfer from scientific institutions to laboratories of small and medium private enterprises. Moreover, an in vitro culture procedure was established for this species, which proved to be extremely effective in virus eradication. Advanced technology and sustainable quality production of high economic value plants are keys for future competitiveness in floriculture.

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“…It was shown that ninety percent of the farmers from the Great Lake Region were able to recognise bunchy top symptoms (Niyongere et al ., ). Moreover, the process of initiating the plants in vitro can eradicate the virus (Lassois et al ., ), further reducing the chance of introducing BBTV into collections. CMV was not detected in our study, neither in pre‐indexing nor indexing steps.…”

Section: Discussionmentioning

confidence: 99%

Lessons learned from the virus indexing of Musa germplasm: insights from a multiyear collaboration

Clerck

Crew

houwe³

et al. 2017

Annals of Applied Biology

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The Bioversity International Transit Center (ITC) for banana hosts more than 1500 accessions largely covering the genetic diversity of the genus Musa. Its objective is to conserve this genetic diversity and to supply plant materials to users worldwide. All the Musa accessions must be tested for virus presence and, if infected, virus elimination must be attempted, to enable the supply of virus-free plant material. An international collaborative effort launched under the auspices of Bioversity International (2007)(2008)(2009)(2010)(2011)(2012)(2013) finally led to the implementation of a two-step process to test the accessions. The first step, called pre-indexing, involved only molecular tests and was designed as a pre-screen of new germplasm lines or existing accessions to reduce the need for post-entry virus therapy and repeated virus indexing. The second step, called full indexing, was performed on either older existing accessions or newer accessions which tested negative during pre-indexing, and involved molecular tests, transmission electron microscopy (TEM) and symptom observation. In total, 270 germplasm lines (434 samples) were pre-indexed; while full indexing was carried out on 243 accessions (68 of which had been pre-indexed). A significant proportion of the samples tested during pre-indexing was infected with at least one virus (68%), showing the utility of this early pre-screening step. Banana streak OL virus and Banana mild mosaic virus were the most commonly detected viruses during both pre-and full indexing. For 22 accessions, viral particles were observed by TEM in full indexing while the molecular tests were negative, underlining the importance of combining various detection techniques. After full indexing, viruses were not detected in 166 accessions, which were then released for international distribution from the ITC. This publication exemplifies how the practical application of diagnostic protocols can raise fundamental questions related to their appropriate use in routine practice and the need for their continuous monitoring and improvement after their first publication.

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Thermotherapy, Chemotherapy, and Meristem Culture in Banana

Cited by 20 publications

References 9 publications

Safeguarding and using global banana diversity: a holistic approach

Safeguarding and using global banana diversity: a holistic approach

RT‐PCR tests for sensitive detection of the major Ranunculus‐infecting viruses: field and in vitro applications

Lessons learned from the virus indexing of Musa germplasm: insights from a multiyear collaboration

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