Plant Tissue Culture and Genetic Transformation for Crop Improvement

Gosal, S. S.; Kang, Manjit S.

doi:10.1002/9783527632930.ch16

Cited by 11 publications

(11 citation statements)

References 132 publications

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“…The production of the highest rate of embryogenic calli is very important for developing an efficient regeneration protocol. That, in turn, can be used as a substantial tool for crop improvement and in functional studies of genes [20]. The maximum number of plantlets of Malaysian rice varieties was regenerated from the highest rate of embryogenic calli [78].…”

Section: Histologymentioning

confidence: 99%

“…Even though these varieties are said to be high yielding their plants are however susceptible to flooding, drought, and low resistance to diseases and pests (MR220, MR232, and Bario) [17][18][19]. To overcome these challenges, conventional breeding, genetic engineering, and in vitro tissue culture methods could be combined to develop new rice varieties [20]. Although conventional breeding methods improve rice varieties, their progress rate is slow [21].…”

Section: Introductionmentioning

confidence: 99%

“…Besides, SE is a rapid and robust system for clonal propagation of many plant species receiving attention for its commercial value [28]. In this process, callus induction and subsequently plant regeneration are the prime steps and prerequisite before employing any genetic improvement program [20,29,30]. The success of SE depends on various factors including genotype, explant, plant growth regulator, basal salt, cultural conditions and developmental stages of the mother plants [31,32].…”

Section: Introductionmentioning

confidence: 99%

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Efficient Callus Induction and Regeneration in Selected Indica Rice

Mostafiz

Wagiran

2018

Agronomy

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An efficient callus induction and in vitro regeneration were developed using plant growth regulators, carbon sources, and basal media for three selected Malaysian wetland rice varieties (MR220, MR220-CL2, and MR232) and one upland variety (Bario). Effect of plant growth regulator (PGR) was carried out using four different concentrations (1-4 mg/L) of 2,4-D (2, 4-dichlorophenoxyacetic acid), and NAA (1-naphthalene acetic acid) (2.5, 5.0, 7.5, and 10 mg/L) with optimized 2,4-D. Effects of carbon sources (maltose and sorbitol), and basal media (MS, N6, and LS) were also studied with optimized PGR to maximize the induction of regenerable calli. This study found that all four varieties exhibited high frequency of callus induction on MS (Murashige and Skoog) medium that was supplemented with 3 mg/L 2,4-D and 30 g/L maltose. Callus induction frequencies in the cases of MR220, MR220-CL2, MR232, and Bario were found to be 76%, 94%, 85%, and 42% respectively. Morphological analysis through scanning electron microscopy (SEM) and histological analysis revealed the embryogenicity of the induced callus. In the regeneration study, it was observed that combination of 2 mg/L BAP (6-benzylaminopurine), 2 mg/L Kin (Kinetin) and 0.5 mg/L NAA supplemented MS medium has the potential to promote regeneration of selected indica rice varieties with higher regeneration percentage, i.e., 82% (MR220-CL2), 68% (both in MR220 and MR232), and 40% (Bario). The optimized conditions for callus formation and regeneration can be useful for biotechnological practices for the genetic improvement of Malaysian indica rice.

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Section: Histologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Efficient Callus Induction and Regeneration in Selected Indica Rice

Mostafiz

Wagiran

2018

Agronomy

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“…Plant tissue culture technique covers a wide scope of development, maintenance and usage of genetic variability to improve field, vegetable, crops and aromatic/medicinal plants. In several crops, in order to develop transgenic varieties, tissue culture-based genetic transformation has been commercially utilized [3]. However, the lack of an In the present study, we have reported a simple, fast and efficient regeneration protocol using mature embryos as explants, and discovered its effective applicability to a range of Indica rice genotypes.…”

Section: Introductionmentioning

confidence: 97%

Highly efficient protocol for callogenesis, somagenesis and regeneration of Indica rice plants

Azizi

Rafii

Mahmood

et al. 2015

Comptes Rendus. Biologies

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In the present study, we have reported a simple, fast and efficient regeneration protocol using mature embryos as explants, and discovered its effective applicability to a range of Indica rice genotypes. We have considered the response of six varieties in the steps of the regeneration procedure. The results showed that calli were variably developed from the scutellar region of seeds and visible within 6-20 days. The highest and lowest calli induction frequency (70% and 51.66%) and number of induced calli from seeds (14 and 10.33) were observed in MR269 and MRQ74, respectively. The maximum and minimum number (7.66 and 4) and frequency of embryogenic calli (38.33% and 20%) were recorded in MR219 and MRQ74, respectively. However, the highest browning rate was observed in MR84 (87%) and the lowest rate in MRQ50 (46%). The majority of plants regenerated from embryogenic calli were obtained from MRQ50 (54%) and the minimum number of plants from MR84. In this study, the maximum numbers of plantlets were regenerated from the varieties with highest rate of embryogenic calli. Also, various varieties, including MRQ50, MR269, MR276 and MR219, were satisfactorily responding, while MRQ74 and MR84 weakly responded to the procedure. Such a simple, successful and generalized method possesses the potential to become an important tool for crop improvement and functional studies of genes in rice as a model monocot plant.

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“…Thus, the production of herbicide resistant garlic plants should help to increase its yield potential. However, despite recent advances (Gosal and Kang, 2012) garlic species have proven to be recalcitrant to genetic transformation techniques such that transgenic garlic plants with agronomic benefits rarely develop to maturity.…”

Section: Introductionmentioning

confidence: 99%

Development of an Efficient Agrobacterium-Mediated Transformation System and Production of Herbicide-Resistant Transgenic Plants in Garlic (Allium sativum L.)

Ahn

Yoon

Jeon

2013

Molecules and Cells

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The genetic improvement of garlic plants (Allium sativum L.) with agronomical beneficial traits is rarely achieved due to the lack of an applicable transformation system. Here, we developed an efficient Agrobacterium-mediated transformation procedure with Danyang, an elite Korean garlic cultivar. Examination of sGFP (synthetic green fluorescence protein) expression revealed that treatment with 2-(N-morpholino) ethanesulfonic acid (MES), L-cysteine and/or dithiothreitol (DTT) gives the highest efficiency in transient gene transfer during Agrobacterium co-cultivation with calli derived from the roots of in vitro plantlets. To increase stable transformation efficiency, a two-step selection was employed on the basis of hygromycin resistance and sGFP expression. Of the hygromycin-resistant calli initially produced, only sGFP-expressing calli were subcultured for selection of transgenic calli. Transgenic plantlets produced from these calli were grown to maturity. The transformation efficiency increased up to 10.6% via our optimized procedure. DNA and RNA gel-blot analysis indicated that transgenic garlic plants stably integrated and expressed the phosphinothricin acetyltransferase (PAT) gene. A herbicide spraying assay demonstrated that transgenic plants of garlic conferred herbicide resistance, whilst nontransgenic plants and weeds died. These results indicate that our transformation system can be efficiently utilized to produce transgenic garlic plants with agronomic benefits.

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Plant Tissue Culture and Genetic Transformation for Crop Improvement

Cited by 11 publications

References 132 publications

Efficient Callus Induction and Regeneration in Selected Indica Rice

Efficient Callus Induction and Regeneration in Selected Indica Rice

Highly efficient protocol for callogenesis, somagenesis and regeneration of Indica rice plants

Development of an Efficient Agrobacterium-Mediated Transformation System and Production of Herbicide-Resistant Transgenic Plants in Garlic (Allium sativum L.)

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