Efficient Callus Induction and Regeneration in Selected Indica Rice

Mostafiz, Suraiya Binte; Wagiran, Alina

doi:10.3390/agronomy8050077

Cited by 70 publications

(62 citation statements)

References 63 publications

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“…In previous study it were showed that the callus induction frequency were between 60% and 90% [22], in the present study the callus induction rate reached up to 72% that support the previous study. However, high callus induction and regeneration may differ in different media composition and varieties [4], [5]. In this study, whitish and globular calli were found in media containing maltose and sucrose treated media formed brownish calli.…”

Section: Discussionmentioning

confidence: 50%

“…In this experiment we used four types of media composition. MS medium containing 30g/L sucrose (T1) [23]; 30g/L Maltose (T2) [5]; 30 g/L Sucrose, 0.5 g/L Proline and 0.5 g/L Glutamine (T3) [24] and 30 g/L Maltose, 0.3 g/L Casein hydrolysate, 0.6 g/L L-proline and 0.25 mg/L BAP (T4). The culture media were autoclaved at 1.16 kgcm-2 pressure and 1210 C temperature for 30 minutes.…”

Section: Callus Inductionmentioning

confidence: 99%

“…On the other hands the farm areas are being lessen by residential areas in the developing countries. Previous experiments showed that the production rate of rice is unpleasantly affected by abiotic stress and that is highly defenseless with the change of weather [5,6,19,3]. Various Modern biotechnological tools requires the optimization of an efficient regeneration protocol for specific cultivar(s) is an essential for the composition of the nutrient media is a major factor that influencing the regeneration frequency of rice [2].…”

Section: Introductionmentioning

confidence: 99%

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Studies on In Vitro Response to Callus Induction and Regeneration of Five High Yielding Indica rice Varieties

Khan

Islam²,

Islam³

2019

JHPR

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Due to growing population, there is an increasing demand of rice production but the productivity of rice is lessened day by day. To overcome this problem various biotechnological tools can be used for developing various rice varieties. However, the lack of a simple and efficient protocol for callus induction, embryogenic callus formation and quick plant regeneration in this cereal crop. In this study embryogenic calli from mature seeds of five indica rice varieties viz. Binadhan-5, Binadhan-6, BRRI dhan-48, BRRI dhan-58 and IR-64 were observed that is done in four different types of media composition. The highest callus induction were observed in media containing Sucrose as a carbon source. Among those varieties Binadhan-6 and BRRI dhan-48 showed highest rate of callus induction respectively. This study will be useful for selecting suitable callus induction medium for callus induction in future that will be useful for not only national but also international plant breeders for producing new variety and so on.

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Section: Discussionmentioning

confidence: 50%

Section: Callus Inductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Studies on In Vitro Response to Callus Induction and Regeneration of Five High Yielding Indica rice Varieties

Khan

Islam²,

Islam³

2019

JHPR

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“…Similar observation using BA in combination with NAA promoting plant regeneration from somatic embryos in rice has been reported (Lee et al 1999(Lee et al , 2002Saha et al 2017). Mostafiz and Wagiran (2018) reported that the medium fortified with 2.0 mg L −1 BA and 0.25 mg L −1 NAA promotes shoot development in rice. The germination of somatic embryos is affected by the subculture of embryogenic callus.…”

Section: Development Of Embryogenic Calli For Plant Transformationmentioning

confidence: 99%

Overexpression of annexin gene in rice (Oryza sativa L.) for salinity and water stress

Mallick

Jadhao

Rout

2020

In Vitro Cell.Dev.Biol.-Plant

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The aim of the study is to develop transgenic rice with annexin genes (AnnBj2) to determine its salinity and water stress tolerance through Agrobacterium-mediated genetic transformation. The transgenic somatic embryos were developed on Murashige and Skoog medium fortified with 0.5 mg L −1 kinetin and 3.0 mg L −1 2,4-D. About 80% of the transformed somatic embryos germinated and was successfully established in the transgenic greenhouse. The transformed plantlets (T 0 and T 1 generation) were identified through PCR using NPT-II and AnnBj2 gene-specific primers as well as positive uidA reporter gene expression. Compared with non-transformed plants, the transgenic rice plants overexpressing AnnBj2 gene exhibited salt tolerance at the seedling stage. Seeds generated at T 0 and T 1 generations were further studied to determine their salinity tolerance through nutrient culture and pot culture experiments for water stress. When compared with the non-transgenic control, transgenic seeds (T 0 and T 1) had increased germination on a nutrient medium having 200 mM NaCl. The data showed that the shoot and root lengths were longer at 200 mM NaCl when compared with those of the non-transformed plants. Further, the biochemical assessment (photosynthetic pigment analysis, protein, proline content, and oxidative enzyme activity) was performed between transformed (T 0 and T 1) and non-transformed plantlets under stress. It was noted that the transformed plantlets were higher in chlorophyll and proline content as well as oxidative enzyme activity as compared with the non-transformed plants.

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“…2,4-D is also used in tissue cultures to induce somatic embryogenesis of plant cells (Gaspar et al, 1996;Mostafiz and Wagiran, 2018). Plant growth regulators such as 2,4-D induce pineapple flowering by increasing the content of ethylene in the meristematic zone of the plant (Burg and Burg, 1966;Abeles, 1968;Grossmann, 2010), where the absorption of products is faster (Cunha, 2005).…”

mentioning

confidence: 99%

Use of 2,4-D in Mixture with Ethephon and Calcium Carbide in Pineapple Crop

et al. 2019

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The 2-Chloroethylphosphonic acid (ethephon) and calcium carbide are used to induce and synchronize the flowering of pineapple crop. Some farmers have used the 2,4-D herbicide in combination with these flowering inducers. This practice is based on the hypothesis that 2,4-D would act as a growth regulator, increasing fruit size and weight. The objectives of this work was to verify if the use of 2,4-D, associated to calcium carbide or ethephon, influences the productivity and quality of the pineapple fruits and, also if the use of this herbicide leaves residues in the fruits. For this, two field experiments were carried out in two different farms. The treatments consisted of the application of ethephon and calcium carbide, alone, and in a mixture with two, four and six drops of 2,4-D in the center of the leaflet of the pineapple cv. “Pérola” at 10 months after planting. The use of 2,4-D mixed with calcium carbide or ethephon, regardless of the dose and culture environment, did not influence the size, weight, total soluble solids content, density and pH of the pineapple fruits. However, depending on the growing environment, ethephon + 2,4-D mixtures did not influence or reduce flowering and crop yield. No 2,4-D residues were found in the fruit pulp of pineapple. It was concluded that the use of 2,4-D as a growth regulator of pineapple under the conditions evaluated does not incorporate qualitative or quantitative benefits to the productive system of this crop. In addition, no 2,4-D residues were found in the fruit pulp of pineapple.

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Efficient Callus Induction and Regeneration in Selected Indica Rice

Cited by 70 publications

References 63 publications

Studies on <i>In Vitro </i>Response to Callus Induction and Regeneration of Five High Yielding <i>Indica </i>rice Varieties

Studies on <i>In Vitro </i>Response to Callus Induction and Regeneration of Five High Yielding <i>Indica </i>rice Varieties

Overexpression of annexin gene in rice (Oryza sativa L.) for salinity and water stress

Use of 2,4-D in Mixture with Ethephon and Calcium Carbide in Pineapple Crop

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