Colorimetric Determination of DDT.Color Test For Related Compounds

Schechter, M. S.; Soloway, Saul; Hayes, Robert A.; Haller, H. L.

doi:10.1021/i560147a010

Cited by 112 publications

(41 citation statements)

References 19 publications

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“…The degradation product formed by the interaction of DDT and alcoholic alkali (2: 2-bis (p-chlorophenyl) -1: 1-dichloroethylene) does not yield a colour with their reagents (unpublished data). Schechter et al (1945) published a specific method for the estimation of DDT and related compounds, depending on intensive nitration followed by the development of colours in benzene solution with methanolic sodium methylate, and subsequent spectrophotometric estimation. This method was later applied to animal tissues by Ofner and Calvery (1945) and by the present writer (unpublished).…”

Section: Discussionmentioning

confidence: 99%

“…The present paper deals with the latter topics in an attempt to find out how DDT acts on living cells as well as on the organism as a whole. METHODS DDT and 2: 2-bis(p-chlorophenyl)acetic acid (DDA) were estimated spectrophotometrically by the method of Schechter, Soloway, Hayes, and Haller (1945). Organically bound chlorine was determined simultaneously by the method of the writer (1948); urinary sulphur partitions by the volumetric benzidine method as modified by Elson, Goulden, and Warren (1945); urinary glycuronic acid by the method of Kapp (1940); urinary nitrogen, plasma protein, and non-protein nitrogen by the microKjeldahl method; blood lactic acid by the method of Barker and Summerson (1941); glucose by the HagedornJensen method or by the technique of Nelson (1944); 'liver glycogen by the method of Good, Kramer, and Somogyi (1933); serum potassium by the method of Hoffman (1937);.…”

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confidence: 99%

“…Tables I and II it is evident that the only notable degradation product is DDA, and that this accumulates in tissues to a small extent. p-Chlorobenzoic acid was sought for, the analytical method used being the production of a rose-red colour by the dinitro compound under the conditions defined by Schechter et al (1945). : DDA is converted into tetranitrocdichlorobenzophenone by intensive nitration, so that this neutral compound can be separated from dinitrochlorobenzoic acid after nitration of the alkali-soluble fraction; no evidence of the formation of chlorobenzoic acid was obtained.…”

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confidence: 99%

“…Since it has been suggested that DDT owes its toxic action to the liberation of HC1 in tissues, evidence was sought that dehydrochlorination did take place. The product of this reaction, dichlorodiphenyl-dichloroethylene, yields dichlorobenzophenone on intensive nitration, which gives a (Schechter et al, 1945). If only part of the DDT were to react, a discrepancy between the value 'of alkalilabile Cl (Gunther, 1945;Judah, 1949) -and the sectrophotometric determination would have arisen.…”

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Studies on the Metabolism and Mode of Action of DDT

Judah¹

1949

British Journal of Pharmacology and Chemotherapy

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Despite the world-wide employment of 2: 2-bis (p-chlorophenyl) -1: 1: 1-trichloroethane (DDT) as an insecticide, comparatively little interest has been shown in its mode of action and few investigations have been carried out on its metabolism, absorption, and excretion in contaminated animals. The present paper deals with the latter topics in an attempt to find out how DDT acts on living cells as well as on the organism as a whole. METHODS DDT and 2: 2-bis(p-chlorophenyl)acetic acid (DDA) were estimated

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Section: Discussionmentioning

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Studies on the Metabolism and Mode of Action of DDT

Judah¹

1949

British Journal of Pharmacology and Chemotherapy

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“…The doses of contact insecticide retained by larvae after spray treatment with DDT were estimated by stripping the insecticide deposits from the larval cuticle immediately after spraying and analysing them, using the method of Schechter, Soloway, Hayes & Haller (1945).…”

Section: Methodsmentioning

confidence: 99%

THE EFFECT OF BODY WEIGHT ON THE RESISTANCE TO INSECTICIDES OF THE LAST‐INSTAR LARVA OF DIATARAXIA OLERACEA L., THE TOMATO MOTH

Way

1954

Annals of Applied Biology

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With 6 Text-figures)At a constant temperature of zqO C. the final larval instar of DMtaraxia oleracea lasts about 10 days, during which its resistance to D D T and y-BHC as contact insecticides progressively increases up to the 5th or 6th day. It then suddenly decreases, this coinciding with cessation of feeding and the beginning of prepupal formation.Between the 2nd and the 6th days the gross body weight of the last-instar larva increases from about 0.27 to 0.65 g. Under the conditions of the experiments, the LD5o of parathion, as a stomach poison, was linearly related to body weight; on the same basis TEPP was slightly less, and lead arsenate, slightly more, toxic to the larger than to the smaller larvae. However, DDT as a stomach or contact insecticide, and y-BHC as a stomach poison were notably less toxic to the larger larvae. For example, the increase in LD5o for an increase in larval body weight of x z was about x I I for D D T as a stomach poison and about x 12 as a contact insecticide.The order of effectiveness of the above insecticides as stomach poisons for the last-instar larva of D. oleracea was parathion > D D T > y-BHC > TEPP = lead arsenate. Zinc fluoarsenate and rotenone were relatively non-toxic. Larvae of D. oleracea were repelled by food leaf treated with an extract of natural pyrethrins.

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