Cluster analysis of AP-PCR generated DNA fingerprints of Vibrio vulnificus isolates from patients fatally infected after consumption of raw oysters

Vickery, Michael C. L.; Harold, N.; Bej, Asim K.

doi:10.1046/j.1472-765x.2000.00707.x

Cited by 13 publications

(3 citation statements)

References 10 publications

(33 reference statements)

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“…Ribotyping V. vulnificus has demonstrated considerable interstrain genetic heterogeneity and is a useful epidemiological tool but is of limited use in distinguishing virulent from less virulent isolates (1,2,10,21). Contour-clamped homogeneous electric field gel electrophoresis and pulsed-field gel electrophoresis (7,21) and random amplified polymorphic DNA analysis (2,10,17,25,26) also have generally failed to differentiate virulent from avirulent strains. In one exception, Warner and Oliver (27) applied random amplified polymorphic DNA analysis to 70 V. vulnificus strains and reported the presence of a ca.…”

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confidence: 99%

Sequence Polymorphism of the 16S rRNA Gene of Vibrio vulnificus Is a Possible Indicator of Strain Virulence

et al. 2003

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Vibrio vulnificus exhibits considerable strain-to-strain variation in virulence. Attempts to associate phenotypic or genotypic characteristics with strain virulence have been largely unsuccessful. Based on a 17-nucleotide difference throughout the sequence of the small subunit 16S rRNA gene, there are two major groups of V. vulnificus designated types A and B. In a survey of the 16S rRNA genotype in 67 V. vulnificus human clinical and nonclinical strains, we determined that the majority of nonclinical isolates are type A (31 of 33) and that there is a statistically significant association between the type B genotype and human clinical strains (26 of 34).

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confidence: 99%

Sequence Polymorphism of the 16S rRNA Gene of Vibrio vulnificus Is a Possible Indicator of Strain Virulence

et al. 2003

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“…For example, noted that phenotypic variation is so great for V. vulnificus such that rapid culture-based identification systems are of little value. Genotypic variability has also been demonstrated using pulsed-field gel electrophoresis, ribotyping, and randomly amplified polymorphic DNA (Tamplin et al, 1996;Vickery et al, 2000;Lin et al, 2003). Nonetheless, d-mannitol reaction is predictive of V. vulnificus genotype for about 85% of the time, suggesting its utility in strain characterization.…”

Section: Discussionmentioning

confidence: 99%

Correlation of Mannitol Fermentation with Virulence-Associated Genotypic Characteristics inVibrio vulnificusIsolates from Oysters and Water Samples in the Gulf of Mexico

Drake

Whitney

Levine

et al. 2010

Foodborne Pathogens and Disease

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Vibrio vulnificus strains (n = 469) isolated from the Gulf of Mexico oysters and waters over a period of 2 years were subjected to phenotypic and genotypic characterizations. Of the strains that could be definitively genotyped (n = 465), 58% were classified as genotype A, 29% as genotype B, and 13% as genotype A/B by 16S rRNA genotyping. When the same strain bank was characterized by virulence-correlated gene (vcg) typing, 65% were genotype E while 35% were genotype C. Further analysis focusing on strains falling into typical genotype categories (i.e., 16S rRNA types A or B, excluding type A/B strains) showed a high degree of concordance (93%) when comparing the two genotyping methods. d-Mannitol fermentation was also predictive of genotype, with an 86% agreement between 16S rRNA genotype and mannitol fermentation patterns, and an 85% agreement between vcg genotype and mannitol fermentation patterns. d-Mannitol fermentation should be considered as a simple and less expensive alternative to screen V. vulnificus isolates for virulence potential, particularly when analyzing large strain banks.

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“…Others confirmed the great degree of diversity seen with RAPD PCR, noting little correlation between strain source and RAPD pattern (Lin and others 2003). The same can be said for arbitrarily primed (AP)‐PCR (Vickery and others 2000). It is also clear that biotype designations do not always correlate with phylogenies generated by molecular typing methods (Gutacker and others 2003).…”

Section: Introductionmentioning

confidence: 95%

An Overview of Vibrio vulnificus and Vibrio parahaemolyticus

Drake¹,

DePaola²,

Jaykus³

2007

Comp Rev Food Sci Food Safe

159

111

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The Vibrionaceae are environmentally ubiquitous to estuarine waters. Two species in particular, V. vulnificus and V. parahaemolyticus, are important human pathogens that are transmitted by the consumption of contaminated molluscan shellfish. This document provides a comprehensive review of the current state of knowledge about these important foodborne disease agents. Topics include the epidemiology of human disease; biotypes and virulence factors; cultural and molecular-based detection methods; phenotyping and genotyping approaches; microbial ecology; and candidate control strategies. Recent international risk assessment efforts are also described. The reader will gain an understanding of why these organisms pose a public health risk and how improving our understanding of their behavior in the environment and the host can aid in reducing that risk in the future.

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Cluster analysis of AP-PCR generated DNA fingerprints of Vibrio vulnificus isolates from patients fatally infected after consumption of raw oysters

Cited by 13 publications

References 10 publications

Sequence Polymorphism of the 16S rRNA Gene of Vibrio vulnificus Is a Possible Indicator of Strain Virulence

Sequence Polymorphism of the 16S rRNA Gene of Vibrio vulnificus Is a Possible Indicator of Strain Virulence

Correlation of Mannitol Fermentation with Virulence-Associated Genotypic Characteristics inVibrio vulnificusIsolates from Oysters and Water Samples in the Gulf of Mexico

An Overview of Vibrio vulnificus and Vibrio parahaemolyticus

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