Sequence Polymorphism of the 16S rRNA Gene of Vibrio vulnificus Is a Possible Indicator of Strain Virulence

Abstract

Vibrio vulnificus exhibits considerable strain-to-strain variation in virulence. Attempts to associate phenotypic or genotypic characteristics with strain virulence have been largely unsuccessful. Based on a 17-nucleotide difference throughout the sequence of the small subunit 16S rRNA gene, there are two major groups of V. vulnificus designated types A and B. In a survey of the 16S rRNA genotype in 67 V. vulnificus human clinical and nonclinical strains, we determined that the majority of nonclinical isolates are type A (31 of 33) and that there is a statistically significant association between the type B genotype and human clinical strains (26 of 34).

Decolorization of synthetic melanoidins-containing wastewater by a bacterial consortium

“…The PCR products were used as template for DNA sequencing with UFUL primer (5 0 -GCC TAA CAC ATG CAA GTC GA-3 0 ) [36] and Bigdye Termination v3.1 cycle sequencing kit. The sequences of 16S rDNA were compared with those available in the GenBank, EMBL and DJB databases using the gapped BLASTN 2.0.5 program through the National Center for Biotechnology Information server.…”

“…The 16S rRNA gene PCR amplified from the isolates using 20F and 802R primers was subjected to DNA sequencing with UFUL internal primers [36]. The sequences were then compared using NCBI BLASTN program.…”

Occurrence of clinical genotype Vibrio vulnificus in clam samples in Mangalore, Southwest coast of India

“…Typing based on 16S rRNA sequences was carried out as described by Nilsson et al (2003). A 492 bp region of the 16S rRNA gene of V. vulnificus was amplified using primers UFUL (5 0 -GCCTAACACATGCAAGTCGA-3 0 ) and URUL (5 0 -CGTATTACCGCGGCTGCTGG-3 0 ).…”

“…Recent independent genotyping studies based on DNA sequence polymorphism at individual genetic loci such as the 16S rRNA (Nilsson et al 2003) and virulence-correlated gene (vcg) (Rosche et al 2005) have also demonstrated that V. vulnificus populations could be significantly grouped as either the clinical or environmental genotype. An analysis of the 16S rRNA gene sequences of V. vulnificus isolated from the environmental and clinical origin is seen to differ by a total of 17 out of 1556 bases, with most of the polymorphism being centered around the first 500 base pairs of the forward strand (Van de Peer et al 1996).…”

“…An analysis of the 16S rRNA gene sequences of V. vulnificus isolated from the environmental and clinical origin is seen to differ by a total of 17 out of 1556 bases, with most of the polymorphism being centered around the first 500 base pairs of the forward strand (Van de Peer et al 1996). A restriction fragment analysis of the polymorphic region has further shown to generate two profiles 16S rRNA typeA or 16S rRNA typeB which significantly correlated respectively to its environmental or clinical origin (Aznar et al 1994;Kim and Jeong 2001;Nilsson et al 2003).…”