Clinical and Biochemical Study of Subretinal Fluid Globulins Examined by Disc Electrophoresis

Riebel, O; Lang, B; Preisová, J

doi:10.1159/000264739

Cited by 3 publications

(3 citation statements)

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“…The pathological changes following retinal detachment include accumulation of macrophages and other cell types, such as retinal pigment epithelial cells in the SRF, leakage from ocular vessels and the degeneration of light receptor cells (10,(12)(13)(14)(15). SRF con-tains components characteristic of both the vitreous body and the blood (10,12,(14)(15)(16)(17)(18)(19). Most biochemical research has focussed on the protein composition of the SRF (16)(17)(18)(19).…”

Section: Discussionmentioning

confidence: 99%

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Fibrinolytic Activity of Subretinal Fluid after Cryopexy

Malukiewicz-Wiśniewska

Kotschy

1999

European Journal of Ophthalmology

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Section: Discussionmentioning

confidence: 99%

“…The total protein concentration of the SRF tends to rise with the duration of the detachment (10,15), possibly indicating increasing permeability of the choroidal capillaries. This suggests the formation of SRF is a dynamic process.…”

Section: Discussionmentioning

confidence: 99%

Fibrinolytic Activity of Subretinal Fluid after Cryopexy

Malukiewicz-Wiśniewska

Kotschy

1999

European Journal of Ophthalmology

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“…As with the subretinal fluid [Riebel et al, 1975], there are no significant differences between the group of males and that of females in relative values of individual globulin fractions in the AH when separated by disc electrophoresis.…”

Section: Resultsmentioning

confidence: 84%

Disc Electrophoresis of Globulin Fractions of Aqueous Humour in Patients with Retinal Detachment

Lang¹,

Riebel²,

Preisová³

1975

Ophthalmic Res

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Aqueous humour and serum taken from 24 patients with retinal detachment have been examined using the technique of disc electrophoresis on polyacrylamide gel. An evaluation of four groups of globulin fractions (post-albumins, transferrin, polyglobulin and macroglobulin) has been made in persentages of the total number of globulins. Significant differences were found in the 4 aqueous humour globulin fractions as compared to the same fractions of the serum. Patients with retinal detachment due to inflammation were characterized by the higher level of the post-albumin fraction and lower level of the transferrin fraction in the aqueous humour, whereas in traumatic cases and in aphakia the conditions were just the opposite. With the duration of the disease, the level of the polyglobulin fraction was enhanced both in the aqueous humour and in the serum.

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A polyacrylamide-gel electrophoretic study of human tear proteins

Berta

1982

Graefe's Arch Clin Exp Ophthalmol

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The protein composition of normal and pathological tears was studied by polyacrylamide-gel (disc) electrophoresis. Polyacrylamide-gel electrophoresis was shown to detect at least 14 fractions in 2-10 microliters of native tears. A comparison was made between the protein composition of normal tears and serum. The most characteristic bands in the tear-protein pattern were identified by parallel electrophoresis of tears, serum, human milk and purified egg-white lysozyme. The identified fractions were specific tear prealbumin, serum albumin, transferrin, lactoferrin and lysozyme. An unidentified major tear-protein component was also described. The tear-protein pattern was divided into six zones: (1) prealbumin zone; (2) post-albumin zone; (3) post-transferrin zone; (4) macroglobulin zone; (5) basic globulin zone; (6) prelactoferrin zone. A significant rise in the level of serum albumin and transferrin was demonstrated in tears from cases of acute catarrhal conjunctivitis. The optimal circumstances were discussed under which major and minor tear components and basic and acidic tear proteins can be determined simultaneously. Polyacrylamide-gel electrophoresis is recommended as a useful method to study the various diseases of the anterior segment of the eye.

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Clinical and Biochemical Study of Subretinal Fluid Globulins Examined by Disc Electrophoresis

Cited by 3 publications

References 1 publication

Fibrinolytic Activity of Subretinal Fluid after Cryopexy

Fibrinolytic Activity of Subretinal Fluid after Cryopexy

Disc Electrophoresis of Globulin Fractions of Aqueous Humour in Patients with Retinal Detachment

A polyacrylamide-gel electrophoretic study of human tear proteins

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