Circulating miR-29b and miR-424 as Prognostic Markers in Patients with Acute Cerebral Infarction

Zhang, Yue-Zhan; Wang, Jun; Xu, Feng

doi:10.7754/clin.lab.2017.170420

Cited by 19 publications

(16 citation statements)

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“…Based on the result of previous studies, ACI can lead to serious sequelae; therefore, it is important to identify novel and sensitive prognostic markers . In the present study, the GOS of the patients was recorded for the evaluation of clinical outcomes, and we found that the plasma levels of miR‐99b were negatively correlated with the GOS of the patients, suggesting that miR‐99b may serve as potential prognostic markers in ACI.…”

Section: Discussionmentioning

confidence: 51%

“…miRNAs were often short in length (about 18-22 nucleotides), and they can negatively regulate the expression of their target genes on post-transcriptional levels. 10,11 The roles of miRNAs in ACI have also been reported in many previous studies, and the diagnostic and prognostic value of some circulating miRNAs, for example, miR-124 and miR-29b have also been discussed. 11,12 The roles of miR-99 miRNA family (including miR-99a, miR-99b and miR-100) in cerebrovascular diseases have been reported previously.…”

Section: Introductionmentioning

confidence: 78%

“…10,11 The roles of miRNAs in ACI have also been reported in many previous studies, and the diagnostic and prognostic value of some circulating miRNAs, for example, miR-124 and miR-29b have also been discussed. 11,12 The roles of miR-99 miRNA family (including miR-99a, miR-99b and miR-100) in cerebrovascular diseases have been reported previously. [13][14][15] Based on the results of a previous animal study, the level of miR-99b in the peripheral blood was down-regulated the brain of rat ACI models, 15 however, whether miR-99b could serve as a plasma diagnostic and prognostic marker remains unclear.…”

Section: Introductionmentioning

confidence: 78%

“…MicroRNAs (miRNAs or miR) belong to the family of the non‐coding RNAs. miRNAs were often short in length (about 18‐22 nucleotides), and they can negatively regulate the expression of their target genes on post‐transcriptional levels . The roles of miRNAs in ACI have also been reported in many previous studies, and the diagnostic and prognostic value of some circulating miRNAs, for example, miR‐124 and miR‐29b have also been discussed …”

Section: Introductionmentioning

confidence: 84%

“…Increasing evidence indicated that circulating microRNAs may serve as diagnostic markers in ACI. For example, Zhou et al reported that plasma levels of both miR‐21 and miR‐24 may function as early diagnostic markers at early stage of ACI; Wang et al reported that miR‐210 may regulate the proliferation and apoptosis of the endothelial cells in ACI; Zhang et al suggested that miR‐29b and miR‐424 may serve as prognostic markers and therapeutic targets for the treatment of ACI . The roles of miR‐99 family in cerebrovascular diseases have been discussed in some previous studies; however, it remains to be examined whether the plasma levels of the miR‐99 family miRNAs were changed in patients with ACI.…”

Section: Discussionmentioning

confidence: 99%

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Plasma level of miR‐99b may serve as potential diagnostic and short‐term prognostic markers in patients with acute cerebral infarction

Zhang

et al. 2020

Clinical Laboratory Analysis

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Objective The aim of the present study is to explore the potential diagnostic and prognostic value of plasma levels of miR‐99 family for patients with acute cerebral infarction (ACI). Methods A total of 112 patients who have been diagnosed with ACI were enrolled in this study, and 112 healthy volunteers were served as the controls. The plasma of the patients and controls were collected, and total RNAs were isolated, and the expression levels of miR‐99a, miR‐99b, and miR‐100 in the plasma of patients and controls were compared determined by RT‐qPCR methods; moreover, the receiver operating characteristic (ROC) curve has been drawn to determine whether the plasma levels of miR‐99b can distinguish patients with ACI from the controls; furthermore, the short‐term prognosis of the patients was evaluated by glasgow outcome scale (GOS), and the correlation between the plasma levels of miR‐99b and the GOS of the patients was evaluated. Finally, the correlation between the plasma level of miR‐99 and VEGF of ACI patients was analyzed. Results It was observed that miR‐99b was significantly decreased in the plasma of ACI patients compared with the healthy controls (P < .01), while the plasma levels of miR‐99a and miR‐100 showed no significant differences between the patients with ACI and the healthy controls; moreover, the area under the curve (AUC) of miR‐99b for the diagnosis of ACI was 0.8882 (95% confidence interval (CI), 0.8451‐0.9313), suggesting that plasma level of miR‐99b is a sensitive marker to distinguish patients with ACI from the healthy volunteers; furthermore, the serum level of miR‐99b was negatively correlated with GOS score of the patients (r = −.56, P < .001); finally, the plasma level of miR‐99b was negatively correlated with the levels of VEGF (r = −.3013, P = .0012). Conclusion miR‐99b was down‐regulated in plasma of patients with ACI, and plasma level of miR‐99b may be a potential diagnostic and prognostic marker for the diagnosis and treatment of ACI.

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Section: Discussionmentioning

confidence: 51%

Section: Introductionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Plasma level of miR‐99b may serve as potential diagnostic and short‐term prognostic markers in patients with acute cerebral infarction

Zhang

et al. 2020

Clinical Laboratory Analysis

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Plasma miR‐409‐3p promotes acute cerebral infarction via suppressing CTRP3

Song

Zhu

2020

The Kaohsiung J of Med Scie

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Abnormal expression of miR-409-3p has been found in several neurodevelopmental disorders, but whether it is dysregulated in the patients with acute cerebral infarction (ACI) has not been evaluated. The current study mainly focused on the clinical significance and the underlying mechanism of plasma miR-409-3p in the progression of ACI. The level of plasma miR-409-3p was determined in ACI patients (n = 80) and healthy controls (n = 30). Pearson correlation assay was performed to evaluate the association and cardiovascular risk factors. A receiver operating characteristic curve (ROC) was used to evaluate the diagnostic value of plasma miR-409-3p levels in patients with ACI. Dual luciferase reporter assay and western blot were performed to determine the possible target gene of miR-409-3p. Our data showed that the expression of plasma miR-409-3p in the ACI group was higher than that in the healthy controls. Furthermore, Pearson correlation analysis indicated a positive correlation between plasma miR-409-3p and the NIHSS score. ROC analysis indicated that plasma miR-409-3p could differentiate plasma miR-409-3p in ACI patients from healthy controls. Then, we explored the possible target genes of miR-409-3p. Interestingly, C1q and TNF-related 3 (CTRP3), a novel adipose tissue-derived secreted factor, was found to be a target gene of miR-409-3p. We found that knockdown of CTRP3 significantly induced PC12 cell apoptosis, even in PC12 cells transfected with miR-409-3p inhibitor. These data suggested that miR-409-3p induced PC12 cell apoptosis by targeting CTRP3. Altogether, elevated plasma miR-409-3p is correlated with disease severity and may be efficient for the early diagnosis of ACI.

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miR-424-5p combined with miR-17-5p has high diagnostic efficacy for endometriosis

Lin¹,

Zeng

2022

Arch Gynecol Obstet

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Purpose: Endometriosis (EMT) is a chronic benign disease with high prevalence. This study investigated the diagnostic value of serum miR-17-5p, miR-424-5p, and their combined expressions for EMT.Methods: A total of 80 EMT patients of reproductive age were included as the study subjects, and another 80 healthy women of reproductive age were selected as the control group. The whole blood samples of enrolled subjects were collected and clinical characteristics were recorded. The miR-17-5p, miR-424-5p, VEGFA, IL-4, and IL-6 levels in the serum were measured. ROC curve was used to evaluate the diagnostic e cacy of miR-17-5p and miR-424-5p expressions for EMT. Pearson correlation was performed to analyze the correlation of miR-17-5p and miR-424-5p with clinical indexes in EMT patients.Results: miR-17-5p and miR-424-5p were signi cantly downregulated in EMT patients. For the diagnosis of EMT, the AUC of miR-17-5p was 0.865 and cutoff value was 0.890 (91.3% sensitivity and 85% speci city), the AUC of miR-424-5p was 0.737 and cutoff value was 0.915 (98.8% sensitivity and 61.2% speci city), the AUC of miR-424-5p combined with miR-17-5p was 0.938 and cutoff value was 2.205 (93.8% sensitivity and 88.7% speci city), with the diagnostic e cacy higher than miR-424-5p or miR-17-5p alone. The expressions of miR-17-5p and miR-424-5p were negatively correlated with dysmenorrhea, infertility, pelvic pain, and rASRM stage, but not with age, BMI, menstrual disorder, and nulliparity. VEGFA, IL-4, and IL-6 were remarkably increased in EMT patients, and both were inversely associated with miR-17-5p and miR-424-5p.Conclusion: miR-424-5p combined with miR-17-5p has high diagnostic e cacy for EMT.

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Circulating miR-29b and miR-424 as Prognostic Markers in Patients with Acute Cerebral Infarction

Cited by 19 publications

References 0 publications

Plasma level of miR‐99b may serve as potential diagnostic and short‐term prognostic markers in patients with acute cerebral infarction

Plasma level of miR‐99b may serve as potential diagnostic and short‐term prognostic markers in patients with acute cerebral infarction

Plasma miR‐409‐3p promotes acute cerebral infarction via suppressing CTRP3

miR-424-5p combined with miR-17-5p has high diagnostic efficacy for endometriosis

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