miR-424-5p combined with miR-17-5p has high diagnostic efficacy for endometriosis

Abstract

Purpose: Endometriosis (EMT) is a chronic benign disease with high prevalence. This study investigated the diagnostic value of serum miR-17-5p, miR-424-5p, and their combined expressions for EMT.Methods: A total of 80 EMT patients of reproductive age were included as the study subjects, and another 80 healthy women of reproductive age were selected as the control group. The whole blood samples of enrolled subjects were collected and clinical characteristics were recorded. The miR-17-5p, miR-424-5p, VEGFA, IL-4, and IL-6 levels in the serum were measured. ROC curve was used to evaluate the diagnostic e cacy of miR-17-5p and miR-424-5p expressions for EMT. Pearson correlation was performed to analyze the correlation of miR-17-5p and miR-424-5p with clinical indexes in EMT patients.Results: miR-17-5p and miR-424-5p were signi cantly downregulated in EMT patients. For the diagnosis of EMT, the AUC of miR-17-5p was 0.865 and cutoff value was 0.890 (91.3% sensitivity and 85% speci city), the AUC of miR-424-5p was 0.737 and cutoff value was 0.915 (98.8% sensitivity and 61.2% speci city), the AUC of miR-424-5p combined with miR-17-5p was 0.938 and cutoff value was 2.205 (93.8% sensitivity and 88.7% speci city), with the diagnostic e cacy higher than miR-424-5p or miR-17-5p alone. The expressions of miR-17-5p and miR-424-5p were negatively correlated with dysmenorrhea, infertility, pelvic pain, and rASRM stage, but not with age, BMI, menstrual disorder, and nulliparity. VEGFA, IL-4, and IL-6 were remarkably increased in EMT patients, and both were inversely associated with miR-17-5p and miR-424-5p.Conclusion: miR-424-5p combined with miR-17-5p has high diagnostic e cacy for EMT.

Plasma microRNA expression in adolescents and young adults with endometriosis: the importance of hormone use

“…This difference could be due to different physiological influences of a natural menstrual cycle and exogenous hormone use on miRNA expression or underpowered analyses given the small sample size included for assessing changes across the menstrual cycle. Further, the majority of previous studies have restricted to participants who did not use hormonal treatments in the past 3 months (18,19,55,56); however, we observed an important modifying or mediating effect of hormone use within our adolescent and young adult population for identifying miRNAs that are associated with endometriosis. As patients with endometriosis symptoms frequently use hormonal treatments before surgical confirmation of their diagnosis, it is important that future studies take into account hormone use for an improved understanding how these diagnostic tests may be utilized in the future.…”

“…Recently research has expanded into elucidating miRNA profiles of endometriosis ( 10 – 12 ), and investigating how changes in miRNA expression may impact gene expression in ectopic endometrial tissue ( 13 , 14 ). While recent studies have reported promising results for the use of miRNA signatures in endometriosis diagnosis ( 15 – 19 ), gaps remain in our understanding of the relationship between miRNAs and endometriosis etiology ( 12 ). In particular, previous studies of miRNA diagnostic potential have been conducted among adult women with endometriosis ( 15 , 16 , 18 – 22 ); however, there is a lack of information regarding miRNA levels among adolescents with endometriosis.…”

Circulating microRNAs as Non-Invasive Biomarkers in Endometriosis Diagnosis—A Systematic Review

“…The diagnosis was made using MRI and/or laparoscopy or laparotomy, and it was confirmed histologically where possible. Fourteen studies included patients with endometriosis stage I to IV based on the ASRM classification [20,25,26,28,30,31,34,[38][39][40][41][42][43]49]. Women with moderate-to-severe endometriosis (stage III to IV) were included in ten studies [22,23,27,37,44,45,47,48,50,51].…”

<span data-contrast="auto">Evaluating the Prognostic Potential of CA-125 and miRNA Levels in Endometriosis: A Narrative Review <span data-ccp-props="{">

“…These miRNAs were inversely related to factors like VEGF, IL-4, IL-6, and CA-125. The study suggested that their combined expression could be a key diagnostic marker for endometriosis [72].…”

“…It emphasizes merging traditional biomarkers like CA-125 with newer ones like miRNAs. According to recent studies, such as those by Lin et al (2023), this integration could improve diagnosis, prognosis, and treatment. It highlights the significance of combining molecular data with clinical insights for a holistic approach to patient care.…”