Chromosome-Mediated mcr-3 Variants in Aeromonas veronii from Chicken Meat

Abstract

Two adjacent colistin resistance gene variants, termed and-like, were identified in the chromosome of an isolate obtained from retail chicken meat. The variants showed 95.20% and 84.19% nucleotide sequence identity, respectively, to from porcine Functional cloning indicated that only conferred polymyxin resistance in both and The -like segment was also observed in other species, including ,, and .

Whole‐genome analysis of multi‐drug‐resistant Aeromonas veronii isolated from diseased discus (Symphysodon discus) imported to Korea

“…One group (27 strains) harboured resistance gene(s) against a class of beta‐lactam antibiotics, while the other group (14 strains) harboured resistance gene(s) against beta‐lactam and tetracycline antibiotics. However, strain Ae52 isolated from goldfish, strain AVNIH1 isolated from chicken meat and our strain 17ISAe carried various ARGs belonging to beta‐lactam, rifampin, antifolate, aminoglycoside, colistin, quinolone, macrolide and phenicol antibiotics, different from ARGs in Aeromonas strains previously reported (Jagoda, Honein, Arulkanthan, Ushio, & Asakawa, ; Ling et al., ).…”

Molecular detection of colistin resistance genes (mcr-1, mcr-2 and mcr-3) in nasal/oropharyngeal and anal/cloacal swabs from pigs and poultry

“…Whereas feces are most commonly considered as the major factor in the transfer and spread of the mcr genes, our data showing significantly higher mcr prevalences in nasal/oropharyngeal swabs than in anal/cloacal swabs suggests that both saliva and respiratory secretions might also play important roles. The identification of mcr variants in Moraxella species ( mcr-1 and mcr-2 ) 21,29,30 and Aeromonas veronii ( mcr-3 ) 24 indicates there are organisms outside the Enterobacteriaceae that contribute to colistin resistance and these might be responsible for the differences in prevalences that we found.…”

“…While colistin is a last-line antibiotic used to treat multidrug resistant Gram-negative bacteria, its efficacy is being compromised by the recently detected mobile colistin resistance genes, mcr-1 3 , and subsequently mcr-2 , mcr-3 , mcr-4 , mcr- 5 11,24,27,28 . Our study has shown that the mcr-1 , mcr-2 and mcr-3 occurred widely in pigs and poultry from China (Table 1, Tables S1–S5).…”

“…coli in China 23 and later shown to be present in bacteria isolated from humans from Denmark, chickens from China, cattle from Spain 19,20,24–26 , and flies in China 31 . Our findings indicate that the gene is quite widespread, as we found it in samples from pigs and poultry from many provinces in China, albeit at low prevalence in some cases.…”

“…The mcr-1 , mcr-2 and mcr-3 were originally described on plasmids in Enterobacteriaceae but have recently been identified on the chromosomes of Moraxella spp. and Aeromonas veronii 21,24,29,30 . mcr-4 and mcr-5 have only been described very recently, after we carried out our study in which we used short amplicon PCRs to determine the prevalence of the mcr-1 , mcr-2 and mcr-3 in swabs from the initial and terminal alimentary system of pigs and poultry in China.…”