Cholestanol and Plant Sterols in the Adrenal Gland of the Rat.

Miettinen, Tatu A.; Albertsson, J.; Alnås, Åke; Hemmingsson, Sten; Kullberg, Lennart; Blinc, R.; Paušak, S.; Ehrenberg, L.; Dumanović, J.

doi:10.3891/acta.chem.scand.21-0286

Cited by 11 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Until recently it was thought that plant sterols are not absorbed through the intestine, but several plant sterols have been detected in human serum (Boehle, Harmuth & Rajewsky, 1964) and Miettinen (1967) has found campesterol, /?sitosterol, other plant sterols and cholestanol in adrenal glands of rats. Miettinen considers that small amounts of plant sterols and cholestanol will probably be found to occur alongside cholesterol in all parts of the body.…”

Section: Discussionmentioning

confidence: 99%

Skin penetration by cercariae of the bird schistosomeAustrobilharzia terrigalensis:the stimulatory effect of cholesterol

1969

Parasitology

View full text Add to dashboard Cite

An artificial skin of hardened gelatine was used to examine the factors affecting penetration of skin by cercariae of the bird schistosome, Austrobilharzia terrigalensis.The percentage of cercariae able to penetrate through a gelatine membrane was increased by a factor of 3–4 by coating the membrane with a thin layer of lipid collected from the surface of chicken skin.The free sterol fraction, isolated from chicken skin surface lipid by thin-layer chromatography, stimulated penetration to the same extent as whole skin lipid.Cholesterol was detected in the sterol fraction by mass spectrometry and pure cholesterol had the full stimulating effect on cercarial penetration.Skin lipid, from which free sterols had been removed, lost the stimulatory effect on cercariae, but full activity was recovered by adding cholesterol to the sterol-free lipid. Fractions of skin lipid containing free fatty acids or triglycerides, wax esters and sterol esters similarly failed to stimulate penetration.These results establish that penetration of A. terrigalensis cercariae is greatly stimulated by the free sterols present in the surface lipid of chicken skin but cholesterol may not be the only active sterol. Cholestanol and the plant sterols campesterol and β-sitosterol were also detected in chicken skin surface lipid. These sterols were not tested for activity on cercariae because samples free from cholesterol could not be obtained.Some cercariae were able to penetrate plain gelatine membranes not coated with cholesterol but small amounts of free sterol were detected in the gelatine itself. This sterol could not be completely removed by prolonged solvent extraction and consequently it is not known whether any cercariae are able to penetrate in the complete absence of sterols.Temperature had a marked effect on penetration of cercariae; lowering the temperature from 40 to 25 °C reduced the number of successful penetrants by a factor of 4.I am grateful to Miss V. Bowen for excellent technical assistance and to Dr J. McLeod of the Research School of Chemistry, Australian National University, who performed and interpreted the mass spectrometry. This investigation was partly supported by a research grant from the World Health Organization.

show abstract

Section: Discussionmentioning

confidence: 99%

Skin penetration by cercariae of the bird schistosomeAustrobilharzia terrigalensis:the stimulatory effect of cholesterol

1969

Parasitology

View full text Add to dashboard Cite

show abstract

Identifizierung der Sterine in verschiedenen Lipidgemischen von Schweinen

Homberg¹

1979

Fette, Seifen, Anstrichm.

View full text Add to dashboard Cite

show abstract

Wirkung langkettiger Monoensäuren auf die Lipide in Nebennieren und Blutserum von Schweinen

Homberg¹,

Bielefeld²,

Seher³

1980

Fette, Seifen, Anstrichm.

View full text Add to dashboard Cite

glycerin-Vollesterfraktionen auftrennen. Die Triglyceride haben den hochsten hRf-Wert, dann folgen die getrennten Cle-l ,Z-Diglycerin-(kleine Fraktion) und Clo-1,l -Diglycerintetraester (grofle Fraktion), die Triglycerinpentaester, die Tetraglycerinhexaester usw. Die von den Polyglycerin 1 -Vollestern zu den Polyglycerin 3-Vollestern (Abb. 9, Start 2-4) starker werdende Fraktion mit dem niedrigsten hRf-Wert schreiben wir den Vollestern der cyclischen Diglycerine zu.S c h l u f l b e m e r k u n g e n Wir haben reine Fettsaure-Polyglycerinpartialcster synthetisiert, mit Hilfe der DC (besonders der zweidimensionalen Arbeitsweise) in charakteristische Chromatogramme mit einer groflen Anzahl von Stoffklassen aufgetrennt und teilweise mit reinen Vergleichssubstanzen identifiziert. Deutlich sind die funf Fettsaure-Partialglyceride, die verschiedenen Fettsaure-Diglycerinpartial-ester und die Fettsaure-Triglycerinpartialester zu erkennen. Diese einfache DC-Methode gibt einen Einblick in den iiberaus komplexen Charakter der Fettsaure-Polyglycerinpartialester.Trotz unserer Bemuhungen bleiben viele Fragen offen. Eine vollstandige Auftrennung der Fettsaure-Polyglycerinpartialester in die Partialester des Glycerins, des Di-, Tri-, Tetraglycerins usw. und deren zahlreiche theoretisch mogliche stellungsisomere Mono-, Di-, Tri-, Tetraester usw. war weder zu erwarten noch unter diesen Bedingungen iiberhaupt nioglich.Fur wertvolle Anregungen und die Diskussion der Ergebnisse dieser Arbeit danken wir herzlich Herrn Prof. Dr. A. Seher von der Bundesanstalt fur Fettforschung in Munster, fur die mit Rat und T a t erwiesene Unterstutzung Herrn Dr. R. Lenck vom Institut fur anorganische und angewandte Chemie der Universitat Hamburg.

show abstract

Cholestanol and Plant Sterols in the Adrenal Gland of the Rat.

Cited by 11 publications

References 0 publications

Skin penetration by cercariae of the bird schistosomeAustrobilharzia terrigalensis:the stimulatory effect of cholesterol

Skin penetration by cercariae of the bird schistosomeAustrobilharzia terrigalensis:the stimulatory effect of cholesterol

Identifizierung der Sterine in verschiedenen Lipidgemischen von Schweinen

Wirkung langkettiger Monoensäuren auf die Lipide in Nebennieren und Blutserum von Schweinen

Contact Info

Product

Resources

About