Chitosan Nanoparticles as a Carrier for &lt;i&gt;Mentha longifolia&lt;/i&gt; Extract:Synthesis, Characterization and Antifungal Activity

Mohamed, Abd El-Aziz Abeer Ramadan; Rashed, Al-Othman Monira; Mahmoud, Mohamed A.; Shehata, Shereen M.; Abdelazim, Nahla Shazli

doi:10.18520/cs/v114/i10/2116-2122

Cited by 33 publications

(4 citation statements)

References 39 publications

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“…According to the results of FTIR analysis, the 3200 cm -1 peak of OH and NH bonds in chitosan shifted to 3200-3600 cm -1 in NPDP nanoparticles. This result indicated that hydrogen bonding is enhanced due to hydrostatic interaction between amino groups and phosphoric groups in TPP (Agarwal et al, 2018;El Aziz et al, 2018). The 1655 cm -1 peak of amide bending vibrations of chitosan shifted to 1693 cm -1 .…”

Section: Discussionmentioning

confidence: 89%

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The combination therapy of targeting both paclitaxel and Dendrophthoe pentandra leaves extract nanoparticles for improvement breast cancer treatment efficacy by reducing TUBB3 and MAP4 expressions

et al. 2021

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The aim of this study is to investigate the combination treatments of paclitaxel and chitosan-Dendrophthoe pentandra leaves extract nanoparticles (NPDP) on MCF-7 breast cancer cells. Chitosan-NPDP nanoparticles were characterized by Fourier-transform infrared (FTIR), scanning electron microscopy (SEM), and assessed by using immunofluorescence microscopy. MCF-7 cells are cultured and divided into six groups: group 1 was a negative control (without paclitaxel or NPDP); group 2 was treated with paclitaxel alone; groups 3-5 were treated with NPDP (2, 4, and 8 mg/mL, respectively) and group 6 was treated only by 8 mg/mL of chitosan-NPDP nanoparticles. The proliferation and cell cycle were analyzed by flow cytometry and the expression of TUBB3 and MAP4 were assessed by immunofluorescence microscopy. The combinations of paclitaxel-NPDP significantly inhibit proliferation of cells (P<0.001) and it is able to induce G2/M cell cycle arrest (P<0.001). The combination of paclitaxel-NPDP significantly decreases the expressions of TUBB3 (P<0.001) and MAP4 (P<0.001) in MCF-7 cells. These results indicate that the combination of NPDP nanoparticles could reduce the expressions of TUBB3 and MAP4. This research may provide possible sources of new therapy for NPDP.

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Section: Discussionmentioning

confidence: 89%

“…(Da Silva et al, 2015). This was due to the aggregation caused by heavy inter-and intra-molecular bonding of hydrogen that could not be stopped even with the homogenization of the vortex (El Aziz et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

The combination therapy of targeting both paclitaxel and Dendrophthoe pentandra leaves extract nanoparticles for improvement breast cancer treatment efficacy by reducing TUBB3 and MAP4 expressions

et al. 2021

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“…In the case of Propionibacterium acnes, these changes were also beneficial. Data indicates the synergism of bactericidal activity of chitosan and the Calendulae flos lyophilized extract against the anaerobic pathogen, which often infects the skin [78,79]. In the last stage of the work, optimization of obtaining a topical pharmaceutical form containing the Calendulae flos lyophilized extract with chitosan 80/500 1:1 (w/w) and 80/1000 1:1 (w/w) was carried out to obtain appropriate adhesive properties for the supporting strategy for wound healing applications.…”

Section: Resultsmentioning

confidence: 99%

Hydrogel Delivery System Containing Calendulae flos Lyophilized Extract with Chitosan as a Supporting Strategy for Wound Healing Applications

et al. 2020

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Calendulae flos is a valued plant material with known anti-inflammatory and antimicrobiological properties. The limitation for its use in the treatment of chronic wounds is the lack of adhesion to the required site of action. Obtaining the Calendulae flos lyophilized extract from water-ethanolic extract allowed to prepare valuable material whose biological activity in the wound healing process was confirmed by a positive result of the scratch test. The Calendulae flos lyophilized extract was standardized for the contents of the chlorogenic acid and the narcissin. The significant potency of the Calendulae flos pharmacological activity has become the reason for studies on its novel applications in combination with the multifunctional chitosan carrier, to create a new, valuable solution in the treatment of chronic wounds. The use of chitosan as a carrier allowed for the controlled release of the chlorogenic acid and the narcissin. These substances, characterized by prolonged release from the chitosan delivery system, were identified as well permeable, based on the results of the studies of the parallel artificial membrane permeability assay (PAMPA Skin) a model simulating permeability through membrane skin. The combination of the Calendulae flos lyophilized extract and the chitosan allowed for synergy of action towards hyaluronidase inhibition and effective microbiological activity. Optimization of the hypromellose hydrogel preparation ensuring the required rheological properties necessary for the release of the chlorogenic acid and the narcissin from the chitosan delivery system, as well as demonstrated antimicrobial activity allows indicating formulations of 3% Calendulae flos lyophilized extract with chitosan 80/500 in weight ratio 1:1 and 2% or 3% hypromellose as an important support with high compliance of response and effectiveness for patients suffering from chronic wounds.

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“…The sample with highest antibacterial and biofilm inhibitory activities was selected for evaluation its effectiveness in a chitosan based nanoformulation. Blank chitosan nanoparticles and chitosan nanoparticles loaded with the plant sample were prepared by ionic gelation method as described earlier [42]. Summarily, a 1 mg ml −1 sodium tripolyphosphate (TPP) (Sigma-Aldrich Co.) solution was prepared in distilled water.…”

Section: Determination Of Minimum Inhibitory Concentrationmentioning

confidence: 99%

Investigation of the antibacterial and biofilm inhibitory activities of Prangos acaulis (DC.) Bornm in nanoparticulated formulation

Nosrati¹,

Ranjbar²

2022

Nanotechnology

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Here in, a chitosan based nanoformulation of P.acaulis was evaluated for its antibacterial and antibiofilm inhibitory activities against some known foodborne bacteria. The FTIR, FE-SEM, DLS and zeta-potential analysis were performed for confirming loading process, morphological appearance, hydrodynamic diameter and surface charge of the nanoparticles respectively. The results confirmed that, the nanoparticles had semi-spherical shape with the mean hydrodynamic diameter and surface charge of 89.8±5.8 nm and 10.78±2.7 mv respectively. Furthermore, the FTIR analysis approved that the nanoparticles were successfully loaded with ethyl acetate fraction from P.acaulis. The antibacterial and biofilm inhibitory activities of the nanoformulated fraction were significantly increased against the tested Gram positive strains than free sample. The results also confirmed that the fraction release from the nanoparticles follows a sustained manner release after 30 h in a logarithmic pattern. Based on the obtained results, chitosan based nanoformulation of P. acaulis can be considered for more evaluations to serve as an alternative natural antibiotic.

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Chitosan Nanoparticles as a Carrier for <i>Mentha longifolia</i> Extract:Synthesis, Characterization and Antifungal Activity

Cited by 33 publications

References 39 publications

The combination therapy of targeting both paclitaxel and Dendrophthoe pentandra leaves extract nanoparticles for improvement breast cancer treatment efficacy by reducing TUBB3 and MAP4 expressions

The combination therapy of targeting both paclitaxel and Dendrophthoe pentandra leaves extract nanoparticles for improvement breast cancer treatment efficacy by reducing TUBB3 and MAP4 expressions

Hydrogel Delivery System Containing Calendulae flos Lyophilized Extract with Chitosan as a Supporting Strategy for Wound Healing Applications

Investigation of the antibacterial and biofilm inhibitory activities of Prangos acaulis (DC.) Bornm in nanoparticulated formulation

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