Investigation of the antibacterial and biofilm inhibitory activities of Prangos acaulis (DC.) Bornm in nanoparticulated formulation

Abstract

Here in, a chitosan based nanoformulation of P.acaulis was evaluated for its antibacterial and antibiofilm inhibitory activities against some known foodborne bacteria. The FTIR, FE-SEM, DLS and zeta-potential analysis were performed for confirming loading process, morphological appearance, hydrodynamic diameter and surface charge of the nanoparticles respectively. The results confirmed that, the nanoparticles had semi-spherical shape with the mean hydrodynamic diameter and surface charge of 89.8±5.8 nm and 10.78±2.7 mv respectively. Furthermore, the FTIR analysis approved that the nanoparticles were successfully loaded with ethyl acetate fraction from P.acaulis. The antibacterial and biofilm inhibitory activities of the nanoformulated fraction were significantly increased against the tested Gram positive strains than free sample. The results also confirmed that the fraction release from the nanoparticles follows a sustained manner release after 30 h in a logarithmic pattern. Based on the obtained results, chitosan based nanoformulation of P. acaulis can be considered for more evaluations to serve as an alternative natural antibiotic.

Exploring Possible Ways to Enhance the Potential and Use of Natural Products through Nanotechnology in the Battle against Biofilms of Foodborne Bacterial Pathogens

“…In contrast, a few nanoparticles were ineffective. For instance, nanoparticles showed MIC values at the microgram level or failed to eradicate preformed biofilms or kill the biofilm residents [ 145 , 191 , 194 ]. Zein nanoparticles loaded with anacardic acid could not kill and destroy the residents of preformed biofilms [ 195 ].…”

“…Moreover, nanoparticles engineered with biofilm-degrading components such as DNase, proteinases, and β-N-acetyl-glucosaminidase, showed better biofilm-eradicating potential [ 201 , 202 ]. Other positively charged nanoparticles show better binding and penetrating potential, possibly through electrostatic interaction with biofilm components [ 146 , 191 , 203 ]. However, a deeper study is required to determine whether this is the actual scenario.…”