Drug delivery strategies for antibiofilm therapy

Choi, Victor; Rohn, Jennifer L.; Stoodley, Paul; Carugo, Dario; Stride, Eleanor

doi:10.1038/s41579-023-00905-2

Cited by 36 publications

(25 citation statements)

References 225 publications

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“…As biofilm is a multilayered entity, it is impermeable to antimicrobial agents, and only a higher concentration of antibiotics can tackle the biofilm infections. 46 Therefore, we first tested the antibiofilm efficacy of G21 to eradicate the biofilms of S. aureus (pCypet-His-expressing cells), P. aeruginosa (mCherry-expressing cells), and C. albicans [green fluorescent protein (GFP)-expressing cells]. The preformed biofilms were treated with G21 1× MIC (8 μg/mL) for 3 h and observed using confocal laser scanning microscopy (CLSM).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…EPS also provides an adaptive environment to develop resistance against antimicrobial agents. As biofilm is a multilayered entity, it is impermeable to antimicrobial agents, and only a higher concentration of antibiotics can tackle the biofilm infections . Therefore, we first tested the antibiofilm efficacy of G21 to eradicate the biofilms of S.…”

Section: Resultsmentioning

confidence: 99%

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Cholic Acid-Derived Gemini Amphiphile Can Eradicate Interkingdom Polymicrobial Biofilms and Wound Infections

Arora,

Singh,

Saini

et al. 2023

ACS Infect. Dis.

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Biofilm infections are mainly caused by Gram-positive bacteria (GPB) like Staphylococcus aureus, Gram-negative bacteria (GNB) like Pseudomonas aeruginosa, and fungi like Candida albicans. These infections are responsible for antimicrobial tolerance, and commensal interactions of these microbes pose a severe threat to chronic infections. Treatment therapies against biofilm infections are limited to eradicating only 20−30% of infections. Here, we present the synthesis of a series of bile acid-derived molecules using lithocholic acid, deoxycholic acid, and cholic acid where two bile acid molecules are tethered through 3′-hydroxyl or 24′-carboxyl terminals with varying spacer length (trimethylene, pentamethylene, octamethylene, and dodecamethylene). Our structure−activity relationship investigations revealed that G21, a cholic acid-derived gemini amphiphile having trimethylene spacer tethered through the C24 position, is a broad-spectrum antimicrobial agent. Biochemical studies witnessed that G21 interacts with negatively charged lipoteichoic acid, lipopolysaccharide, and phosphatidylcholine moieties of GPB, GNB, and fungi and disrupts the microbial cell membranes. We further demonstrated that G21 can eradicate polymicrobial biofilms and wound infections and prevent bacteria and fungi from developing drug resistance. Therefore, our findings revealed the potential of G21 as a versatile antimicrobial agent capable of effectively targeting polymicrobial biofilms and wound infections, suggesting that it is a promising antimicrobial agent for future applications.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Cholic Acid-Derived Gemini Amphiphile Can Eradicate Interkingdom Polymicrobial Biofilms and Wound Infections

Arora,

Singh,

Saini

et al. 2023

ACS Infect. Dis.

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“…[174] Numerous other examples exist, such as Chen's team utilizing hydrogenated phosphatidylcholine and (1, 2dioleoxy propyl) trimethyl ammonium chloride (DOTAP) to coat 2'3'-cGAMP for enhanced immune effects. [175] Liu's team developed phosphatidylserine-coated liposomes containing 2'3'-cGAMP, which can be rapidly inhaled by mice and effectively delivered to the lung. [176] Exosomes have been extensively investigated as drug carriers, and McAndrews' team has successfully utilized them to deliver STING agonists, achieving superior immune effects compared to free drugs.…”

Section: Advances In Drug Delivery Systems For Sting Agonistsmentioning

confidence: 99%

Chemical Biology Perspectives on STING Agonists as Tumor Immunotherapy

Xuan,

2023

ChemMedChem

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Stimulator of interferon genes (STING) is a crucial adaptor protein in the innate immune response. STING activation triggers cytokine secretion, including type Ⅰ interferon and initiates T cell‐mediated adaptive immunity. The activated immune system converts "cold tumors" into "hot tumors" that are highly responsive to T cells by recruiting them to the tumor microenvironment, ultimately leading to potent and long‐lasting anti‐tumor effects. Unlike most immune checkpoint inhibitors, STING agonists represent a groundbreaking class of innate immune agonists that hold great potential for effectively targeting various cancer populations and are poised to become a blockbuster in tumor immunotherapy. This review will focus on the correlation between the STING signaling pathway and tumor immunity, as well as explore the impact of STING activation on other biological processes. Ultimately, we will summarize the development and optimization of STING agonists from a medicinal chemistry perspective, evaluate their potential in cancer therapy, and identify possible challenges for future advancement.

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“…Drug-resistant bacterial infections are a major global health problem. − Bacterial infections consist of three main phases: initial bacterial adhesion, biofilm formation, and infection. , Most persistent bacterial infections are caused by biofilms, which are composed of bacterial communities encapsulated by drug-resistant autocrine extracellular polymers (EPS) that include components such as polysaccharides, proteins, and extracellular DNA. The special process of biofilm formation leads to a microenvironment that is different from that of other tissues and acidic in character (pH 4.5–6.5), due to sugar fermentation .…”

Section: Introductionmentioning

confidence: 99%

Antibacterial MoS₂/CaCO₃ Nanoplatform for Combined Photothermal, Photodynamic, and Nitric Oxide Therapy

Jin,

Wu,

et al. 2023

ACS Appl. Nano Mater.

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Wound infections affect the health of nearly 300 million people worldwide, and biofilm formation is a major factor that contributes to their persistence. In this study, we designed a combined photothermal therapy (PTT)/photodynamic therapy (PDT)/nitric oxide (NO) antibacterial nanoplatform (MSC@CaCO3). First, photothermally responsive molybdenum disulfide (MoS2) nanospheres (90–110 nm) were prepared, then sodium nitroxide alginate (SANO) and dihydroporphyrin e6 (Ce6) were attached to allow the release of NO and reactive oxygen species (ROS) in response to illumination. While acid-sensitive calcium carbonate comes into contact with the microacidic environment of biofilm, it will crack and release drugs, and free Ca2+ can assist sodium alginate (SA) to promote wound healing. The results showed that the photothermal conversion efficiency of the nanoplatform was 48.9%. The bacterial inhibition rates of MSC@CaCO3 for multidrug-resistant (MDR) Escherichia coli (E. coli) and MDR Staphylococcus aureus (S. aureus) were both more than 96%. MSC@CaCO3 also showed effective inhibition for the growth of drug-resistant bacterial biofilm. A single-factor experiment was carried out to assess the bacterial inhibition efficiency by PTT or PDT. The mouse wound infection model recovered after 10 days of treatment by MSC@CaCO3 under illumination. The toxicity assay demonstrated that the nanoplatform had no significant toxicity. In conclusion, the prepared MSC@CaCO3 nanoplatform can effectively utilize the characteristics of the biofilm microenvironment to confer protection against drug-resistant bacterial infections and promote wound healing. This study provides a basic reference for the application of cost-effective and multifunctional nano-anti-infective drugs.

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Drug delivery strategies for antibiofilm therapy

Cited by 36 publications

References 225 publications

Cholic Acid-Derived Gemini Amphiphile Can Eradicate Interkingdom Polymicrobial Biofilms and Wound Infections

Cholic Acid-Derived Gemini Amphiphile Can Eradicate Interkingdom Polymicrobial Biofilms and Wound Infections

Chemical Biology Perspectives on STING Agonists as Tumor Immunotherapy

Antibacterial MoS₂/CaCO₃ Nanoplatform for Combined Photothermal, Photodynamic, and Nitric Oxide Therapy

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Drug delivery strategies for antibiofilm therapy

Cited by 36 publications

References 225 publications

Cholic Acid-Derived Gemini Amphiphile Can Eradicate Interkingdom Polymicrobial Biofilms and Wound Infections

Cholic Acid-Derived Gemini Amphiphile Can Eradicate Interkingdom Polymicrobial Biofilms and Wound Infections

Chemical Biology Perspectives on STING Agonists as Tumor Immunotherapy

Antibacterial MoS2/CaCO3 Nanoplatform for Combined Photothermal, Photodynamic, and Nitric Oxide Therapy

Contact Info

Product

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About

Antibacterial MoS₂/CaCO₃ Nanoplatform for Combined Photothermal, Photodynamic, and Nitric Oxide Therapy