Chitosan microspheres and sponges: Preparation and characterization

Denkbaş, Emir Baki; Odabaşı, Mehmet

doi:10.1002/(sici)1097-4628(20000613)76:11<1637::aid-app4>3.3.co;2-h

Cited by 32 publications

(46 citation statements)

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“…These results (Table 9) confi rm the fi ndings of another study (19) about the possible application of antimicrobial activity of chitosan. Chitosan due to its polycationic nature can interact with negatively charged microbial cell walls and plasma membranes, resulting in decreased osmotic stability, membrane disruption and eventual leak- Table 9.…”

Section: Antimicrobial Activity Of Herb and Spice Extractssupporting

confidence: 83%

“…Besides their unique benefi ts, liposomes show some disadvantages such as low stability, low encapsulation effi ciency, high cost of manufacturing, degradation by hydrolysis or oxidation, sedimentation, aggregation or fusion during storage (18). Thus, other natural polymers based on polysaccharides have been tested as delivery systems for antimicrobial agents (19,20).…”

Section: Effect Of Encapsulation On Antimicrobial Activity Of Herbal mentioning

confidence: 99%

“…Alginate, chitosan and starch particles were prepared by the emulsifi cation method based on gelation and cross-linking of polymers (15,19,20). Sodium alginate is a water-soluble polymer that forms a gel in the presence of multivalent cations such as calcium.…”

Section: Preparation Of Polysaccharide Particlesmentioning

confidence: 99%

“…Chitosan particles were prepared in a similar way as the alginate ones. Methods proposed to prepare chitosan nanoparticles are based on the spontaneous formation of complexes between chitosan and polyanions such as tripolyphosphate (19). Starch nanoparticles were formed using simple, fast and easy method of nanoprecipitation in absolute ethanol under controlled conditions (20).…”

Section: Preparation Of Polysaccharide Particlesmentioning

confidence: 99%

“…Nanoscale chitosan that has a higher surface-to-volume ratio, resulting in higher surface charge density, leads to increased affi nity towards bacteria and fungi and greater antimicrobial activity. Therefore, it is suitable for encapsulation of antimicrobial ingredients (19).…”

Section: Antimicrobial Activity Of Herb and Spice Extractsmentioning

confidence: 99%

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Effect of Encapsulation on Antimicrobial Activity of Herbal Extracts with Lysozyme

Matoušková

Márová

Bokrová

et al. 2016

Food Technol. Biotechnol.

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SummaryResistance of microorganisms to antibiotics has increased. The use of natural components with antimicrobial properties can be of great signifi cance to reduce this problem. The presented work is focused on the study of the eff ect of encapsulation of selected plant and animal antimicrobial substances (herbs, spices, lysozyme and nisin) on their activity and stability. Antimicrobial components were packaged into liposomes and polysaccharide particles (alginate, chitosan and starch). Antimicrobial activity was tested against two Gram-positive (Bacillus subtilis and Micrococcus luteus) and two Gram-negative (Escherichia coli and Serratia marcescens) bacteria. Encapsulation was successful in all types of polysaccharide particles and liposomes. The prepared particles exhibited very good long-term stability, especially in aqueous conditions. Antimicrobial activity was retained in all types of particles. Liposomes with encapsulated herb and spice extracts exhibited very good inhibitory eff ect against all tested bacterial strains. Most of herbal extracts had very good antimicrobial eff ect against the tested Gram-negative bacterial strains, while Gram-positive bacteria were more sensitive to lysozyme particles. Thus, particles with co-encapsulated herbs and lysozyme are more active against diff erent types of bacteria, and more stable and more eff ective during long-term storage. Particles with encapsulated mixture of selected plant extracts and lysozyme could be used as complex antimicrobial preparation with controlled release in the production of food and food supplements, pharmaceutical and cosmetic industries.

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Section: Antimicrobial Activity Of Herb and Spice Extractssupporting

confidence: 83%

Section: Effect Of Encapsulation On Antimicrobial Activity Of Herbal mentioning

confidence: 99%

Section: Preparation Of Polysaccharide Particlesmentioning

confidence: 99%

Section: Preparation Of Polysaccharide Particlesmentioning

confidence: 99%

Section: Antimicrobial Activity Of Herb and Spice Extractsmentioning

confidence: 99%

See 3 more Smart Citations

Effect of Encapsulation on Antimicrobial Activity of Herbal Extracts with Lysozyme

Matoušková

Márová

Bokrová

et al. 2016

Food Technol. Biotechnol.

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Human serum albumin (HSA) adsorption with chitosan microspheres

Denkbaş

Odabaşı

Kılıçay

et al. 2002

J of Applied Polymer Sci

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ABSTRACT:In this study, chitosan microspheres were prepared and characterized for adsorption of human serum albumin (HSA) as affinity sorbent. The chitosan microspheres were obtained with a "suspension crosslinking technique" in the size range of 30 -700 m by using a crosslinker, i.e., glutaraldehyde. The chitosan microspheres used in HSA adsorption studies were having the average size of 170 Ϯ 81 m. Adsorption medium pH and the initial HSA concentration in the adsorption medium were changed as 4.0 -7.0 and 0.5-2.0 mg HSA/mL, respectively, to investigate the HSA adsorption capacity of chitosan microspheres. Maximum HSA adsorption (i.e., 11.35 mg HSA/g chitosan microspheres) was obtained at pH 5.0 and 1.5 mg HSA/mL of the initial HSA concentration in the adsorption medium was obtained as the saturation value for HSA adsorption. A very common dye ligand, i.e., Cibacron Blue F3GA was attached to the chitosan microspheres to increase the HSA adsorption capacity. Actually, the HSA adsorption capacity was increased up to 15.35 mg HSA/g chitosan microspheres in the case of Cibacron Blue F3GA attached to chitosan microspheres used.

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Evaluating chitosan/β‐tricalcium phosphate/poly(methyl methacrylate) cement composites as bone‐repairing materials

Kuo

Chang

Lin

et al. 2003

J of Applied Polymer Sci

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This study related to the preparation of chitosan microspheres by means of reacting chitosan with ␤-tricalcium phosphate (␤-TCP) and glutaraldehyde by crosslinking reaction in the oil phase, followed by de-oil and purification processes to get the product. Three cement composites, Pure P, C1P1, and C2P1, were prepared by the polymerization of poly(methyl methacrylate) (PMMA) bone cement in the presence of 0, 50, and 66.7% chitosan/␤-TCP microspheres, respectively. The result revealed the chitosan/␤-TCP microspheres obtained was in the size range of 50 -150 m. The presence of chitosan/␤-TCP microspheres in the prepared composites decreased the ultimate tensile strength, whereas the modulus remained the same as compared with the commercial PMMA bone cement. Addition of chitosan/␤-TCP microspheres into commercial PMMA cement significantly improved the handling property of the cement paste-that is, the increased setting time and less stickiness behavior of this paste was beneficial, in manipulation, to the operation and easier fittings to the shape and gap of the bony defect and interface. The decreased curing temperature was also less harmful to the surrounding tissues. From scanning electron micrograph observations, chitosan/␤-TCP microspheres can completely mix with bone cement powder and the prepared composites could provide scaffold for osteoblast cells growth and thus improve defects of commercial PMMA bone cement.

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Chitosan microspheres and sponges: Preparation and characterization

Cited by 32 publications

References 0 publications

Effect of Encapsulation on Antimicrobial Activity of Herbal Extracts with Lysozyme

Effect of Encapsulation on Antimicrobial Activity of Herbal Extracts with Lysozyme

Human serum albumin (HSA) adsorption with chitosan microspheres

Evaluating chitosan/β‐tricalcium phosphate/poly(methyl methacrylate) cement composites as bone‐repairing materials

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