Human serum albumin (HSA) adsorption with chitosan microspheres

Abstract

ABSTRACT:In this study, chitosan microspheres were prepared and characterized for adsorption of human serum albumin (HSA) as affinity sorbent. The chitosan microspheres were obtained with a "suspension crosslinking technique" in the size range of 30 -700 m by using a crosslinker, i.e., glutaraldehyde. The chitosan microspheres used in HSA adsorption studies were having the average size of 170 Ϯ 81 m. Adsorption medium pH and the initial HSA concentration in the adsorption medium were changed as 4.0 -7.0 and 0.5-2.0 mg HSA/mL, respectively, to investigate the HSA adsorption capacity of chitosan microspheres. Maximum HSA adsorption (i.e., 11.35 mg HSA/g chitosan microspheres) was obtained at pH 5.0 and 1.5 mg HSA/mL of the initial HSA concentration in the adsorption medium was obtained as the saturation value for HSA adsorption. A very common dye ligand, i.e., Cibacron Blue F3GA was attached to the chitosan microspheres to increase the HSA adsorption capacity. Actually, the HSA adsorption capacity was increased up to 15.35 mg HSA/g chitosan microspheres in the case of Cibacron Blue F3GA attached to chitosan microspheres used.

A novel method to prepare chitosan powder and its application in cellulase immobilization

“…In this section we study the correlation between the morphology and the bioimmobilization performance of chitosan. The data in Tables 1 and 2 show that immobilized cellulase using the two types of support evidently demonstrate good results, and the amount of cellulase bound to the support is fairly high (17–150 mg g −1 ) compared with literature values (3–50 mg g −1 ) 20–23. It is revealed that chitosan with smaller pore or particle size possesses more entrances to entrap enzyme than conventional chitosan support with large pore or particle sizes ( ca 500–300 µm), which leads to much improved bioimmobilization ability.…”

“…However, the immobilization behavior (bound capacity) of chitosan may vary significantly according to different methods. Furthermore, considering the large particle size ( ca 500–300 µm) of conventional chitosans,19, 20 their specific capacity for protein still remains poor (<50 mg g −1 support)20, 21 and, moreover, the activity of immobilized cellulase is fairly low 22, 23…”

Chitosan microspheres as immobilized dye affinity support for catalase adsorption

“…On the other hand, silica has much higher mechanical stability than agarose but is unstable against alkaline solution [11]. Therefore, investigators are still trying to develop novel chromatographic support materials and chitosan is one of them [12].…”

Chitosan Sub-micron Particles Prepared Using Sulfate Ion Salt as Bacteriostatic Materials in Neutral pH Condition

“…Thus, the molding, investigation and application of chitosan have been restricted. The methods for producing porous and spherical chitosan microbeads, such as the "suspension evaporation method" [6] and the "suspension crosslinking technique" [9,21] using chitosan acid aqueous solutions, have been reported. These methods require the use of organic solvents and emulsifier.…”

“…Chitosan and derivatives have become useful polysaccharides in the biomedical field. Especially, these microparticles have been utilized as chromatographic packings [5,6], enzyme-immobilized support [7,8], affinity adsorbents for proteins [9], endotoxin adsorbents [10] and drug carriers [11,12]. Generally, it was popular to use chitosan as antibacterial compounds in agriculture, as elicitors of plant defense responses [13], as additives in the food industry, as flocculating agents for wastewater [14] and as pharmaceutical agents in biomedicine [15,16].…”