2005
DOI: 10.1016/j.ijbiomac.2005.08.004
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Chitosan microspheres as immobilized dye affinity support for catalase adsorption

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Cited by 54 publications
(27 citation statements)
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“…It was shown that the process consisted of two steps and properties of nanocomposite were mainly dependent on structure of the material. In 2005, the adsorption of catalase was studied by Shentu et al, [2] using Cibacron Blue F3GA-attached chitosan microspheres (CB-CS). Significant amount of the adsorbed CAT (up to 90.6%) was eluted in the elution medium and it revealed that CB-CS could be applied for adsorption of CAT without causing any denaturation.…”
Section: Chitosan Membranes Reported In Literaturesmentioning
confidence: 99%
See 1 more Smart Citation
“…It was shown that the process consisted of two steps and properties of nanocomposite were mainly dependent on structure of the material. In 2005, the adsorption of catalase was studied by Shentu et al, [2] using Cibacron Blue F3GA-attached chitosan microspheres (CB-CS). Significant amount of the adsorbed CAT (up to 90.6%) was eluted in the elution medium and it revealed that CB-CS could be applied for adsorption of CAT without causing any denaturation.…”
Section: Chitosan Membranes Reported In Literaturesmentioning
confidence: 99%
“…Such isolation or purification of biomolecules involves either adsorption or immobilization of biomolecules onto matrices that bearing functional groups [2]. Among these, the adsorption methods are considered to be ineffective due to weak bonding and low degree of stabilization [3].…”
Section: Introductionmentioning
confidence: 99%
“…Due to its molecular structure, featuring the presence of amino (-NH 2 ) and hydroxyl (-OH) groups, chitosan displays a high affinity for many classes of compounds and has been widely used for the removal of transitions metal ions [14][15][16][17][18][19][20][21][22][23][24][25], dyes and inks [26][27][28][29][30][31][32][33][34][35][36][37], lysozime [38], proteins [39], cholesterol [40], urea [41,42] and several other organic compounds [43][44][45][46][47][48][49]. Chitosan can also be chemically modified to produce fibers, composites and supports, improving its adsorption capacity and offering potential applications in several fields [18-24, 29, 38-44, 49, 50].…”
Section: Introductionmentioning
confidence: 99%
“…The relatively high selectivity, stability and low cost of small dye-ligands make them suitable as affinity ligands to purify proteins in large-scale purification processes [10][11][12][13][14][15][16]. Dyeligands are commercially available, inexpensive and can easily be attached, especially on matrices having hydroxyl groups.…”
Section: Introductionmentioning
confidence: 99%