Chemiluminometric method for the determination of glycerol in wine by flow-injection analysis with co-immobilized glycerol dehydrogenase/NADH oxidase

Amperometric biosensor based on glycerol oxidase for glycerol determination

“…They are based on enzymes of glycerol dehydrogenase [4,10] and glycerol kinase co-immobilized with glycerol-3-phosphate oxidase [2]. The latter was short of stability: after three-day storage in a working buffer only 10% enzymes remained active.…”

“…The total value of glycerol generated at must fermentation is 1/10 to 1/15 of alcohol end concentration, which is 1-10 g/l [2]. Glycerol considerably influences wine flavor imparting oiliness and mildness [2][3][4]. Therefore, the data on the glycerol presence and its volume in alcohol beverages are significant characteristics of their quality.…”

Amperometric Biosensors for Lactate, Alcohols, and Glycerol Assays in Clinical Diagnostics

“…They are based on the use of GDH (Kiba et al, 1996;Laurinavicius et al, 1996) and GK coimmobilized with GPOx (Compagnone et al, 1998). The latter sensor showed insufficient stability: after three days of storage in a working buffer only 10 % of the enzymes remained active.…”

Flow injection chemiluminescent assays for glycerol and triglycerides using a co‐immobilized enzyme reactor

“…The use of immobilized enzyme mini-columns using flow injection analysis (FIA) has emerged as a powerful technique for rapid and automated quantitative analysis and is particularly suited to monitoring chemiluminescent (CL) reactions. FI-CL methods have been reported for glycerol determination in wines, using glycerol dehydrogenase and NADH oxidase co-immobilized on polyvinyl alcohol beads and glycerokinase and glycerol-3-phosphate oxidase co-immobilized on controlled porosity glass with a limit of detection of 7 Â 10 À8 mol/ dm 3 and 12 mg/L, respectively (23,24). An enzymatic biosensor method has been designed for glycerol using covalently immobilized glycerokinase and glycerol-3-phosphate oxidase in conjunction with a platinum base H 2 O 2 probe with a detection limit of 5 Â 10 À7 mol/l.…”