Flow injection chemiluminescent assays for glycerol and triglycerides using a co‐immobilized enzyme reactor

Abstract

A flow injection method for the determination of glycerol using a co-immobilized enzyme reactor containing glycerokinase and glycerol-3-phosphate oxidase is described. The hydrogen peroxide produced is monitored by using a luminol chemiluminescence reaction in the presence of catalyst such as Co(II). The detection limit (2.5 x blank noise) for glycerol is 7 x 10(-3) mmol/L with a sample throughput of 40/h. The calibration graph is linear over the range studied (0.2-1.0 mmol/L) with relative standard deviation 1.2-2.4%. The method is applied to the determination of glycerol in blood serum produced off-line from triglycerides using lipase isolated from bovine pancreas.

Lab-on-a-chip for analysis of triglycerides based on a replaceable enzyme carrier using magnetic beads

“…To the best of our knowledge, there is no literature on TG determination in enzyme-based microchip. The proposed system demonstrated improvements over previous designs, 7,8 particularly with regards to online degradation of TG with replaceable magnetic enzyme carrier. The preparation details, characterization of Lipase-GK-GPOx/chitosan/Fe 3 O 4 composite, and the performance of this composite for TG determination are reported in the following sections.…”

“…Among the various methods available for TG determination, the use of immobilized enzyme mini-columns using flow injection analysis (FIA) 7,8 has emerged as a powerful technique for rapid and automated quantitative analysis. Efforts are being made to immobilize enzymes on the glass beads 8 and the modified capillaries.…”

“…Among the various methods available for TG determination, the use of immobilized enzyme mini-columns using flow injection analysis (FIA) 7,8 has emerged as a powerful technique for rapid and automated quantitative analysis. Efforts are being made to immobilize enzymes on the glass beads 8 and the modified capillaries. However, the serious issue for immobilization on glass beads is the difficulty in controlling the position and the size of the patch of immobilized enzymes, and for immobilization on the modified capillary often leads to time-consuming preparation, non-specific adsorption, poor reproducibility and the inability to renew the enzyme activity.…”