Lab-on-a-chip for analysis of triglycerides based on a replaceable enzyme carrier using magnetic beads

Chen, Shao-Peng; Yu, Xiao-Dong; Xu, Jing‐Juan; Chen, Hong‐Yuan

doi:10.1039/c0an00231c

Cited by 20 publications

(10 citation statements)

References 40 publications

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“…Electrochemical detection of proteins was realized using enzyme-coated magnetic beads. S. P. Chen et al 84 showed detection of triglyceride (TG), in particular of triolein, using this method. First, enzyme-loaded magnetic beads were trapped in a microfluidic channel under a magnetic field.…”

Section: Electrochemical Detectionmentioning

confidence: 99%

Ultrasensitive protein detection: a case for microfluidic magnetic bead-based assays

2013

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We review the use of magnetic micro- and nanoparticles ('magnetic beads') in microfluidic systems for ultrasensitive protein detection. During recent years magnetic beads have been used frequently in immunoassays, either as mobile substrates on which the target antigen is captured, as detection labels, or simultaneously as substrates and labels. The major part of the reviewed work has as application the detection of antibodies or disease biomarkers in serum or of biotoxins from food samples. Several of the most sensitive assays allow protein detection down to fg mL(-1) concentrations. We benchmark the performance of these microfluidic magnetic bead-based assays with the most promising earlier work and with alternative solutions.

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Section: Electrochemical Detectionmentioning

confidence: 99%

Ultrasensitive protein detection: a case for microfluidic magnetic bead-based assays

2013

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“…Triglycerides can indicate the risk of developing conditions such as coronary heart disease and hypertension. 164 Chen et al 167 analyzed serum triglycerides using a microfluidic bead-based enzymatic carrier chip. Enzymes (lipase, glycerokinase, and glycerol-3-phosphate oxidase) co-immobilised on magnetic nanoparticles reacted with triglycerides in serum samples, with electrochemical detection occurring at a gold nanoband microelectrode.…”

Section: Lipidsmentioning

confidence: 99%

Microfluidic platforms for biomarker analysis

et al. 2014

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Biomarkers have been described as characteristics, most often molecular, that provide information about biological states, whether normal, pathological, or therapeutically modified. They hold great potential to assist diagnosis and prognosis, monitor disease, and assess therapeutic effectiveness. While a few biomarkers are routinely utilised clinically, these only reflect a very small percentage of all biomarkers discovered. Numerous factors contribute to the slow uptake of these new biomarkers, with challenges faced throughout the biomarker development pipeline. Microfluidics offers two important opportunities to the field of biomarkers: firstly, it can address some of these developmental obstacles, and secondly, it can provide the precise and complex platform required to bridge the gap between biomarker research and the biomarker-based analytical device market. Indeed, adoption of microfluidics has provided a new avenue for advancement, promoting clinical utilisation of both biomarkers and their analytical platforms. This review will discuss biomarkers and outline microfluidic platforms developed for biomarker analysis.

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“…Chen et al 88 reported an enzyme-magnetic NP-based micro-uidic device for analysis of triglycerides. A gold nanoband microelectrode was developed as an electrochemical detector for triglyceride determination by co-immobilizing lipase, glycerokinase and glycerol-3-phosphate oxidase on chitosan-Fe 3 O 4 composite nanoparticles with a shell-core structure.…”

Section: Nanoparticle-based Enzyme-assay For Signal Amplication In M...mentioning

confidence: 99%

Nanoparticle-based signal generation and amplification in microfluidic devices for bioanalysis

Yue

Yang

2013

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Signal generation and amplification based on nanomaterials and microfluidic techniques have both attracted considerable attention separately due to the demands for ultrasensitive and high-throughput detection of biomolecules. This article reviews the latest development of signal amplification strategies based on nanoparticles for bioanalysis and their integration and applications in microfluidic systems. The applications of nanoparticles in bioanalysis were categorized based on the different approaches of signal amplification, and the microfluidic techniques were summarized based on cell analysis and biomolecule detection with a focus on the integration of nanoparticle-based amplification in microfluidic devices for ultrasensitive bioanalysis. The advantages and limitations of the combination of nanoparticles-based amplification with microfluidic techniques were evaluated, and the possible developments for future research were discussed.

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Lab-on-a-chip for analysis of triglycerides based on a replaceable enzyme carrier using magnetic beads

Cited by 20 publications

References 40 publications

Ultrasensitive protein detection: a case for microfluidic magnetic bead-based assays

Ultrasensitive protein detection: a case for microfluidic magnetic bead-based assays

Microfluidic platforms for biomarker analysis

Nanoparticle-based signal generation and amplification in microfluidic devices for bioanalysis

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