Enzymes in Flow Injection Analysis

Koncki, Robert; Tymecki, Łukasz; Rozum, Beata

doi:10.1002/9783527623259.ch14

Cited by 2 publications

(1 citation statement)

References 89 publications

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“…Such an approach is useful not only for enzyme activity measurements, but also for the detection of respective substrates as well as other species involved in the biocatalytic process, including inhibitors and activators. 1 Moreover, the same methods could be easily adapted for the detection of enzymatic labels often used in various immuno-and geno-assays. In this context the development of novel methods and devices for the detection of p-nitrophenol (NP) is of special significance because this compound is a product of many reactions catalyzed by several hydrolases.…”

Section: Introductionmentioning

confidence: 99%

UV-PEDD photometry dedicated for bioanalytical uses

Tymecki

Brodacka

Rozum

et al. 2009

Analyst

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Detection of p-nitrophenol, a product of several enzymatic reactions, is useful and important in many fields of modern bioanalytics. Here, we have developed and applied a complete photometer dedicated for such measurements that is extremely economic and easily customized for specific experimental setups. The instrument consists only of paired emitter-detector diodes (PEDDs) coupled with an extremely simple and low-cost signal transduction system. The experimental data confirmed that this photometric system adapted for cuvette as well as flow measurements can be applied for fast and reproducible determination of alkaline phosphatase (ALP) activity.

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Section: Introductionmentioning

confidence: 99%

UV-PEDD photometry dedicated for bioanalytical uses

Tymecki

Brodacka

Rozum

et al. 2009

Analyst

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Enzymatic Kinetic Determinations

Hüttl

Frank

2000

Encyclopedia of Analytical Chemistry

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Kinetic methods for analytical applications can be divided into noncatalytic and catalytic methods. Enzyme kinetic determinations are a special type of catalytic methods. Enzymatic reactions are used analytically to determine enzyme activities – for instance, in the diagnosis of diseases, substrate concentrations in the food industry or medicine, and concentrations of effectors – for example, in trace analysis. Owing to the high selectivity of enzymatic analysis, the importance of this method in various fields is growing rapidly. The widest use is observed in clinical chemistry and food chemistry. This article gives only a general survey of the high potential of enzymatic analysis, with references to more detailed literature. Enzymatic reactions are characterized by some special features, explained after the introduction. The theoretical background for kinetic determination is the comprehension of the principles on enzymatic kinetics. In Section 3, the simplest mechanism, outlined by Michaelis and Menten, and several transformations of the equation are described. In this section, two kinetic parameters, enzyme activity and the Michaelis constant, are introduced. Section 4 deals with substances, the so‐called effectors , influencing the rate of an enzymatic reaction. Simple mechanisms of these effectors are shown. Different methods for determination of substrates, enzymes, and effectors, illustrated by many examples, are represented in Section 5. While substrate concentrations can be determined using both equilibrium or kinetic methods, the determination of enzymes and effectors can be performed only by kinetic approaches. In the last two sections, the application of enzymes in flow systems and biosensor development are shown.

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Enzymes in Flow Injection Analysis

Cited by 2 publications

References 89 publications

UV-PEDD photometry dedicated for bioanalytical uses

UV-PEDD photometry dedicated for bioanalytical uses

Enzymatic Kinetic Determinations

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