UV-PEDD photometry dedicated for bioanalytical uses

Tymecki, Łukasz; Brodacka, Luiza; Rozum, Beata; Koncki, Robert

doi:10.1039/b822025e

Cited by 29 publications

(12 citation statements)

References 9 publications

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“…The current literature and research results have established LEDs as visible light/optical [11][12][13] and diagnostic X-ray [14] detectors. In applications [11][12][13], two LEDs were employed (one LED as a light emitter and the other as a detector of the light emitted by the first LED) in the form of a paired emitter detector diode (PEDD). Besides, the comparison of blue and red LED emission and sensitivity spectra discovered drift of the LED detection sensitivity to shorter wavelengths-for both the tested LEDs [11].…”

Section: Introductionmentioning

confidence: 99%

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Amplification of Radiation-Induced Signal of LED Strip by Increasing Number of LED Chips and Using Amplifier Board

et al. 2020

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Transducers, such as photodiodes, phototransistors, and photovoltaic cells are promising radiation detectors. However, for accurate radiation detection and dosimetry, signals that emanate from these devices have to be sufficient to facilitate accurate calibrations, i.e., assigning a quantity of radiation dose to a specific magnitude of the signal. More so, purposely fabricated for luminescence, LEDs produce significantly low signals during radiation detection applications. Therefore, this paper investigates the enhancement and augmentation of photovoltaic signals that were generated when LED strips were being exposed to diagnostic X-rays. Initially, signal amplification was achieved through increasing the effective LED active area (from 60 to 120 chips); by successively connecting LED strips. Further, signal amplification was undertaken by injecting the raw LED strip signal into an amplifier board with adjustable gains. In both the signal amplification techniques, the tube voltage (kVp), tube current-time product (mAs), and source-to-detector distance (SDD) were varied. The principal findings show that effective active area-based signal amplifications produced an overall average of 91.16% signal enhancement throughout all of the X-ray parameter variations. On the other hand, the amplifier board produced an average of 36.48% signal enhancement for the signals that were injected into it. Chip number increment-based signal amplifications had a 0.687% less coefficient of variation than amplifier board signal amplifications. The amplifier board signal amplifications were impaired by factors, such as dark currents, amplifier board maximum operational output voltage, and saturation. Therefore, future electronic signal amplification could use amplifier boards having low dark currents and high operational voltage headroom. The low-cost and simplicity that are associated with active-area amplification could be further exploited in a hybrid amplification technique with electronic amplification and scintillators.

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Section: Introductionmentioning

confidence: 99%

“…In the other sensitivity enhancement approach, the LED sensitive/active area was increased by increasing the number of LED chips. In both sensitivity augmentation methods, our LEDs were in the photovoltaic configuration-similar to elsewhere [12,13]. Stated otherwise, we did not apply any biasing voltage to the LEDs.…”

Section: Introductionmentioning

confidence: 99%

Amplification of Radiation-Induced Signal of LED Strip by Increasing Number of LED Chips and Using Amplifier Board

et al. 2020

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“…As shown elsewhere [22, 23], PEDDs are easily applicable as flow-through detectors in various flow analysis systems. Thus, PEDD-based detectors could be easily miniaturized and integrated with separation chromatographic or electrophoretic devices potentially leading to dedicated analytical tools useful for determination of selected Hb fractions recognized as specific markers for diabetes [24, 25], thalassemia [26, 27], etc.…”

Section: Discussionmentioning

confidence: 99%

Hemoglobin determination with paired emitter detector diode

2010

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Two ordinary green light-emitting diodes used as light emitter and detector coupled with simple voltmeter form a complete, cost-effective prototype of a photometric hemoglobinometer. The device has been optimized for cuvette assays of total hemoglobin (Hb) in diluted blood using three different chemical methods recommended for the needs of clinical analysis (namely Drabkin, lauryl sulfate, and dithionite methods). The utility of developed device for real analytics has been validated by the assays of total Hb content in human blood. The results of analysis are fully compatible with those obtained using clinically recommended method and clinical analyzer.

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“…The electromotive force (EMF) signal generated by the sensor was recorded using an ordinary multimeter from Voltcraft (model VC820, Germany) connected via an RS-232 interface to data storage PC. The use of low internal resistance voltmeter offers significant enhancement of sensitivity [8–10]. …”

Section: Methodsmentioning

confidence: 99%

Miniaturized optical chemosensor for flow-based assays

et al. 2010

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A cost-effective, highly compact, and versatile optoelectronic device constructed of two ordinary light emitting diodes compatible with optosensing films has been developed. This fibreless device containing chemoreceptor, semiconductor light source, and detector integrated in a miniaturized flow-through cell of low microliter internal volume works as a complete photometric chemical sensor suitable for detection in flow analysis. The operation of the developed device under nonstationary programmable-flow conditions offered by sequential injection analysis has been demonstrated using Prussian Blue film as a model optical chemoreceptor. The unique spectroelectrochemical properties of the sensing material enable its use for optical sensing of redox species, whereby ascorbic acid and hydrogen peroxide have been chosen as model analytes. The reported SI-sensor system features fast and reproducible determination of both analytes in the submillimolar range of concentrations. The construction concept demonstrated in this work can be easily applied to other kinds of optical sensors based on absorbance sensing films.Electronic supplementary materialThe online version of this article (doi:10.1007/s00216-010-4384-2) contains supplementary material, which is available to authorized users.

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UV-PEDD photometry dedicated for bioanalytical uses

Cited by 29 publications

References 9 publications

Amplification of Radiation-Induced Signal of LED Strip by Increasing Number of LED Chips and Using Amplifier Board

Amplification of Radiation-Induced Signal of LED Strip by Increasing Number of LED Chips and Using Amplifier Board

Hemoglobin determination with paired emitter detector diode

Miniaturized optical chemosensor for flow-based assays

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