Hemoglobin determination with paired emitter detector diode

Abstract

Two ordinary green light-emitting diodes used as light emitter and detector coupled with simple voltmeter form a complete, cost-effective prototype of a photometric hemoglobinometer. The device has been optimized for cuvette assays of total hemoglobin (Hb) in diluted blood using three different chemical methods recommended for the needs of clinical analysis (namely Drabkin, lauryl sulfate, and dithionite methods). The utility of developed device for real analytics has been validated by the assays of total Hb content in human blood. The results of analysis are fully compatible with those obtained using clinically recommended method and clinical analyzer.

Comparison of three noninvasive methods for hemoglobin screening of blood donors

“…SLS dissolves lipoproteins of the cell membrane of the red blood cells to release Hb and convert it into SLS‐Hb. The concentration of SLS‐Hb is measured as light absorbance at 555 nm . The test was performed as stated in the manufacturer's manual…”

Broadly Available Imaging Devices Enable High-Quality Low-Cost Photometry

“…[20][21][22][23][24][25][26][27][28][29] For example, we and others have constructed low-cost photometers based on this principle to measure i) antibodies against HIV in serum, 21 ii) hemoglobin [22][23] in blood, and iii) fluoride 27 and mercury 29 in water samples.…”

Comparison of noninvasive and invasive point‐of‐care testing methods with reference method for hemoglobin measurement

“…Lipoproteins of the cell membrane of the red blood cells are dissolved with SLS to release Hb and are converted into SLS‐Hb. The measurement of the concentration of SLS‐Hb is performed as light absorbance at 555 nm . The measuring range of the venous method is between 0 and 25 g/dL.…”