Miniaturized optical chemosensor for flow-based assays

Abstract

A cost-effective, highly compact, and versatile optoelectronic device constructed of two ordinary light emitting diodes compatible with optosensing films has been developed. This fibreless device containing chemoreceptor, semiconductor light source, and detector integrated in a miniaturized flow-through cell of low microliter internal volume works as a complete photometric chemical sensor suitable for detection in flow analysis. The operation of the developed device under nonstationary programmable-flow conditions offered by sequential injection analysis has been demonstrated using Prussian Blue film as a model optical chemoreceptor. The unique spectroelectrochemical properties of the sensing material enable its use for optical sensing of redox species, whereby ascorbic acid and hydrogen peroxide have been chosen as model analytes. The reported SI-sensor system features fast and reproducible determination of both analytes in the submillimolar range of concentrations. The construction concept demonstrated in this work can be easily applied to other kinds of optical sensors based on absorbance sensing films.Electronic supplementary materialThe online version of this article (doi:10.1007/s00216-010-4384-2) contains supplementary material, which is available to authorized users.

Photoinduced Anisotropy in Poly(Methyl Methacrylate)/Sudan Red III Thin Films

“…[16][17][18][19] In particular, considerable attention has been paid to the assembly of organic molecules into polymeric thin films as a result of their potential applications for miniaturized optoelectronic devices. [20,21] In azopolymers the photoisomerization induces conformational changes in the polymer chains, which, in turn, leads to macroscopic variations in the chemical and physical properties of their surroundings and media. The photoisomerization allows systems containing azo molecules to be employed as photoswitches that could rapidly and reversibly control the material properties.…”

A New Microfluidic Polymer Chip with an Embedded Cationic Surfactant Ion-selective Optode as a Detector for the Determination of Cationic Surfactants

“…11,12,[14][15][16][17] On the other hand, ion-selective optical sensor (Optode) membranes are suited for a detector of microfluidic chips, compared with the ISE and the ISFET. [18][19][20][21][22][23][24] From the viewpoints of applications to microfluidic technology, optodes are superior to ISEs because they are not subject to electrical noise, and no contacts to microfluidic chips are required for measurements. Furthermore, no reference electrode is needed for measurements using optodes.…”

“…[20][21][22][23][24] Flow-injection analysis (FIA) is one of the analytical methods utilizing a flow system originated by Ruzicka et al In the FIA, after a sample solution is injected into a carrier solution, continuously flowed by a pump and mixed with a reagent solution, a target component in the sample is quantified by a peak signal obtained by a downstream detector. The FIA has attracted much attention as a rapid analytical method capable of precisely controlling the mixing and reaction between a reagent solution and a sample solution in a flow system.…”

Towards optoelectronic urea biosensors

“…Recently, several photometric PEDD-based gas sensors based on immobilized pH indicators useful for detection of acidic vapors [ 32 , 33 ], sweat [ 34 ], and carbon dioxide [ 35 ] have been developed. Another example is an optoelectronic flow-through redox sensor based on chemosensitive Prussian blue film, useful for determination of ascorbic acid and hydrogen peroxide [ 36 ]. Finally, also the first prototypes of fluorimetric PEDD-based sensors dedicated for the detection of riboflavin [ 27 ] and oxygen [ 37 ] have been demonstrated.…”