Chemical constituents and in vitro biological activities of Eremurus spectabilis leaves

Abstract

In the present study, phytochemical investigation of the young leaves of Eremurus spectabilis let to the isolation six compounds (1-6). Their structure was determined by spectroscopic analysis and comparison with the literature data. All compounds were isolated for the first time from E. spectabilis. Isolated compounds and prepared extracts did not show cytotoxic activity against HeLa (Human cervix adenocarcinoma), A-549 (Human alveolar adenocarcinoma), MCF-7 (Human breast adenocarcinoma), mPANC96 (Human pancreas adenocarcinoma), U87MG (Glioblastoma), PC3 (Prostate), CaCo-2 (Colon), HEK293 (Human embryonic kidney cells) cell lines. RAW 264.7 (murine macrophage cell lines) was used to determine the inhibition levels of inducible nitric oxide synthase (iNOS).The hexane extract was found to be active in terms of inhibition of iNOS (IC: 25 μg/ml).

Gastroprotective and antioxidant effects of Eremurus spectabilis Bieb. methanol extract and its isolated component isoorientin on indomethacin induced gastric ulcers in rats

“…Isoorientin was isolated from EtOAc extract via Sephadex LH-20 column eluting with MeOH and then silica gel column eluting with CH 2 Cl 2 : MeOH: H 2 O (90:10:1→50:50:5). The structure of isoorientin was elucidated by 1 H NMR, 13 C NMR, 2D NMR and Q-TOF by Sezen Karaoğlan et al 14 .…”

“…Carvone, carvacrol, pentane, (E)-caryophyllene, valencene were found to be as main constituents of the essential oil of E. spectabilis 12 . It is reported that inosine, methyl linolenate, chrysophanol, isoorientin, β-sitosterol and sucrose were also isolated from E. spectabilis 13 . In a previous study, we isolated a high amount of isoorientin from E. spectabilis 14 .…”

Phytochemical and pharmacological analysis of certain plants applied in the Iranian and European folk medicine

“…The phytochemical composition of Eremurus species have been rarely investigated. In general, isoorientin and methylnaphthalene derivatives have been identified as the predominant phytoconstituents of the plants in this genus [141][142][143][144]. All the isolated compounds are new in E. persicus.…”

“…extracts has been rarely carried out. The following compounds have been previously isolated from Eremurus species: aloesaponol III 8-methyl ether, chrysophanol 8-methyl ether, 2-acetyl-1-hydroxy-8-methoxy-3-methylnaphthalene [141][142][143], methyl linolenate, β-sitosterol, isoorientin, inosine [144], chrysophanol [141][142][143][144], altaicusin A, emodin [142], a bi-anthraquinone glycoside, 2-acetyl-1,8-dimethoxy-3-methylnaphthalene, a pre-anthraquinone, 10-(chrysophanol-7'-yl)-10-hydroxychrysophanol-9-anthrone [141], aloesaponol III, and (R)-aloechrysone [143]. By using Folin-Ciocalteu's method, total phenolic contents of various extract of E. spectabilis [10,145,146] were also assessed.…”

“…Antioxidant and antiradical activities of leaves and roots [10,145,146,155,156], anti-proliferative effects [10,151,156,157], cytotoxic, nitric oxide (NO) production inhibitory activity [144], gastroprotective effect [158], and antibacterial potential [139,145,156] of E. spectabilis extracts have been documented in the literature. Analysis of antioxidant potency of root extract of E. chinensis Fedtch.…”