Chemical Constituents and Antimicrobial Activity of the Leaf Essential Oil of Garcinia kola Heckel (Clusiaceae) from Nigeria

Okhale, Samuel; Buba, Chukkol Ismail; Oladosu, Peters; Egharevba, Henry Omoregie; Ugbabe, G. E.; Ibrahim, Jemilat Aliyu; Kunle, Olobayo O.

doi:10.9734/acsj/2016/24019

Cited by 15 publications

(17 citation statements)

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“…A 1% w/v solution of the sample was prepared and 1 µL was injected using a splitless injection technique. Identification of components was achieved based on their retention time (RT), and by comparison of their mass spectral fragmentation patterns with those reported values in the literature [20] and stored in the MS library [NIST database (G1036A, revision D.01.00)/Chem Station data system (G1701CA, version C.00.01.08)].…”

Section: Gas Chromatography-mass Spectrometry (Gc-ms) Analysesmentioning

confidence: 99%

The GC–MS analysis of the diethylether and ethylacetate fraction of the peel of Solanum incanum and the study of their antibacterial activity

Ravichandran

Yetayih

2022

ADV TRADIT MED (ADTM)

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Section: Gas Chromatography-mass Spectrometry (Gc-ms) Analysesmentioning

confidence: 99%

The GC–MS analysis of the diethylether and ethylacetate fraction of the peel of Solanum incanum and the study of their antibacterial activity

Ravichandran

Yetayih

2022

ADV TRADIT MED (ADTM)

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“…The methods of Okhale et al (2018) were used where fresh C. zambesicus leaf sample was chopped into pieces and subjected to hydro-distillation for 4 h using Clevenger-type apparatus. The essential oil obtained was dried over a hydrous sodium sulphate and used immediately for GC-MS using Shimadzu QP-2010 GC with QP-2010 mass selective detector [MSD, operated in the EI mode (electron energy =70Ev), scan range =45400 amu, and scan rate = 3.99 scan/s], and Shimadzu GCMS solution data system).…”

Section: Extraction Of Oils By Hydro Distillationmentioning

confidence: 99%

“…The essential oil obtained was dried over a hydrous sodium sulphate and used immediately for GC-MS using Shimadzu QP-2010 GC with QP-2010 mass selective detector [MSD, operated in the EI mode (electron energy =70Ev), scan range =45400 amu, and scan rate = 3.99 scan/s], and Shimadzu GCMS solution data system). The percentage of each component was reported as raw percentages based on the total ion current (Okhale et al, 2018).…”

Section: Extraction Of Oils By Hydro Distillationmentioning

confidence: 99%

Leaf microscopy, high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analyses of Croton zambesicus Mll.-Arg. leaf (Family: Euphorbiaceae) in Nigeria

Ugbabe,

Dalyop

2022

Afr. J. Biotechnol.

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This study was done to get information on the chemical composition and the internal structure of the plant which could aid in its further characterization. The study is aimed at determining the epidermal leaf anatomy, HPLC of the hot water extract, chemical constituents of the oil from the leaves by GC-MS and chemo-microscopic properties of Croton zambesicus for proper identification of the plant. The leaf epidermal microscopy of C. zambesicus showed abundant trichome on the abaxial surface arising from a multi-cellular base and radiating radially and paracytic stomata were observed on the lower surface of the leaf. Chemo-microscopic analysis of the leaves showed the presence of cellulose, lignin, starch, oxalate crystals, tannin, oils, proteins and absence of mucilage. The physicochemical parameters evaluated on the leaves had moisture content of 4.10% and total ash value of 11.91%. HPLC analysis of the hot water extract revealed 12 peaks with number 3 having the highest peak of 93 mAU at 3.5 min. The GC-MS analysis of the oil of the leaves had 57 components. Caryophyllene had the highest percentage composition (15.53%) followed by Copaene (11.38%), Phellandrene (8.65%), 1,6-Octadien-3ol, 3,7-dimethyl (4.67%), Humulene (3.94%), Pinene (3.85%) and Ar-tumerone (3.41%).

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“…Individual constituents were identified by comparing their mass spectra with NIST Mass Spectral Library (NIST 08). The percentages of each component are reported as raw percentages based on the total ion current without standardization (Okhale et al, 2018).…”

Section: Gas Chromatography-mass Spectrometry (Gc-ms)mentioning

confidence: 99%

Bioactivity of Essential Oils of Laggera pterodonta and Laggera aurita against Larvae of Anopheles gambiae, Malaria Vector

Dantanko

Malann

2020

The 1st International Electronic Conference on Plant Science

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The role of plants as sources of biologically active entities cannot be overstated. Plants belonging to different families with mosquitocidal activity have been discovered in established ethnobotanical and laboratory-based studies. Biological control of mosquitoes using botanicals remains the safest and most environmentally sound alternative to chemical control. The essential oil of two Nigerian Laggera species, L. pterodonta and L. aurita, were investigated with regard to their ability to kill the fourth instar larvae of the malaria vector, Anopheles gambiae. The WHO protocol was adopted for the larvicidal bioassay. Three replicates comprising 20 larvae each were exposed to various concentrations of the essential oil. Larval mortality was observed after 24 and 48 h, respectively. The results show that mortality increased with an increase in concentration and period of exposure. The essential oil of L. pterodonta was found to be the most effective, with LC50 values of 418 and 404 mg/L after 24 and 48 h, respectively, while the essential oil of L. aurita recorded LC50 value of 688 and 642 mg/L after 24 and 48 h, respectively. The GC-MS results reveal that the essential oil of L. pterodonta comprises 50.83% of compounds that have been reported to have larvicidal activity while the essential oil of L. aurita comprises of 43.69% compounds with larvicidal activity. The better activity of L. pterodonta essential oil could be attributed to it having a higher percentage of compounds such as ɤ-terpinene and 4-carvomenthenol with larvicidal activity. The results suggest that the essential oil of the plants have the potential to be used as an eco-friendly approach for the control of mosquitoes.

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Chemical Constituents and Antimicrobial Activity of the Leaf Essential Oil of Garcinia kola Heckel (Clusiaceae) from Nigeria

Cited by 15 publications

References 0 publications

The GC–MS analysis of the diethylether and ethylacetate fraction of the peel of Solanum incanum and the study of their antibacterial activity

The GC–MS analysis of the diethylether and ethylacetate fraction of the peel of Solanum incanum and the study of their antibacterial activity

Leaf microscopy, high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analyses of Croton zambesicus Mll.-Arg. leaf (Family: Euphorbiaceae) in Nigeria

Bioactivity of Essential Oils of Laggera pterodonta and Laggera aurita against Larvae of Anopheles gambiae, Malaria Vector

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