The GC–MS analysis of the diethylether and ethylacetate fraction of the peel of Solanum incanum and the study of their antibacterial activity

Ravichandran, Y. Dominic; Yetayih, Mequanint Molla

doi:10.1007/s13596-021-00623-1

Cited by 5 publications

(7 citation statements)

References 12 publications

(7 reference statements)

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“…As an explanation, this is unquestionably an oversimplification, and additional mechanisms are almost surely at work. The production of the lactamase enzyme in the periplasmic gap between the thin outer membrane and the cytoplasmic membrane causes Gram-negative bacteria to become resistant to antibiotics like penicillin [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

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Chemical Composition and Antibacterial and Antioxidant Activities of Stem Bark Essential Oil and Extracts of Solanecio gigas

Yitayeh

Wassihun

2022

Biochemistry Research International

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Herbal medication developed from natural resources has to have antibacterial and antioxidant effects. The aim of this research is to look at the chemical makeup of Solanecio gigas (S. gigas) stem bark essential oil (EO), as well as the effectiveness of EO and extracts (chloroform, ethyl acetate, and methanol) against human pathogenic bacteria and their antioxidant activity. The GC-MS analysis identified 23 components, accounting for 98.7% of the total oil containing Methylene chloride (49.2%), sabinene (10.5%), 1-nonene (11.3%), Terpinen-4-ol (6.9%), Camphene (4.3%), γ-terpinene (3.6%), α-phellandrene (2.9%) β-myrcene (2.6%), 1,2,5-Oxadiazol-3-carboxamide, 4,4′-azobis-2,2′-dioxide (2.4%), α-terpinene (1.9%), 1-Octanamine, N-methyl- (1.9%), ρ-cymene (1.6%) as major components. The antibacterial efficacy of the EO and extracts (25, 50, 100, and 200 mg/ml) was demonstrated by the inhibitory zones (8.5 ± 0.47–23.3 ± 0.36 and 7.2 ± 0.25–22.0 ± 0.45 mm), respectively. The MIC values of the extracts and the EO were 120–150 and 240 to <1100 μg/ml, respectively. The EO also demonstrated a significant antibacterial impact. The EO and methanolic extract had free radical scavenging activities with IC50 value, 13.8 ± 0.48 and 4.2 ± 0.04 μg/ml, respectively. In comparison to the other extracts, the methanolic extract had the greatest phenolics (100.2 ± 0.13 μg GAE/mg of dry extract) and flavonoid contents (112.1 ± 0.18 μg CE/mg of dry extract).

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Section: Discussionmentioning

confidence: 99%

“…A Shimadzu GC-MS (GC-17A) equipped with a ZB-1 MS fused silica capillary column (30 m × 0.25 mm i.d., film thickness of 0.25 μ m) was used to analyze the EO [ 14 ]. An electron ionization device with ionization energy of 70 eV was employed for GC-MS detection.…”

Section: Methodsmentioning

confidence: 99%

Chemical Composition and Antibacterial and Antioxidant Activities of Stem Bark Essential Oil and Extracts of Solanecio gigas

Yitayeh

Wassihun

2022

Biochemistry Research International

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“…However, it is found as an end product in substances secreted by different animals as a defense mechanism or for behavioral purposes [ 33 , 34 ]. It is also found in extracts of different plants and is considered to have antimicrobial properties [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Kocuria flava, a Bacterial Endophyte of the Marine Macroalga Bryopsis plumosa, Emits 8-Nonenoic Acid Which Inhibits the Aquaculture Pathogen Saprolegnia parasitica

Deutsch,

Samara,

Nasser

et al. 2023

Marine Drugs

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Secondary metabolites—organic compounds that are often bioactive—produced by endophytes, among others, provide a selective advantage by increasing the organism’s survivability. Secondary metabolites mediate the symbiotic relationship between endophytes and their host, potentially providing the host with tolerance to, and protection against biotic and abiotic stressors. Secondary metabolites can be secreted as a dissolved substance or emitted as a volatile. In a previous study, we isolated bioactive endophytes from several macroalgae and tested them in vitro for their ability to inhibit major disease-causing pathogens of aquatic animals in the aquaculture industry. One endophyte (isolate Abp5, K. flava) inhibited and killed, in vitro, the pathogen Saprolegnia parasitica, an oomycete that causes saprolegniasis—a disease affecting a wide range of aquatic animals. Here, using analytical chemistry tools, we found that Abp5 produces the volatile organic compound (VOC) 8-nonenoic acid. Once we confirmed the production of this compound by the endophyte, we tested the compound’s ability to treat S. parasitica in in vitro and in vivo bioassays. In the latter, we found that 5 mg/L of the compound improves the survival of larvae challenged with S. parasitica by 54.5%. Our isolation and characterization of the VOC emitted by the endophytic K. flava establish the groundwork for future studies of endophytic biocontrol agents from macroalgae. Use of this compound could enable managing oomycete agricultural pathogens in general, and S. parasitica in particular, a major causal agent in aquaculture diseases.

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“…Some minor constituents of the gel extract (see Table S1) have also been reported in literature to possess potential antimicrobial activity. Among this compounds are: pentadecanoic acid [44], hexadecanoic acid, methyl ester [45], phytol [46], 8-nonenoic acid [47], tetradecane [48], n-decanoic acid [49], stevioside [50], dodecane [51], eicosane [52], 2-furanmethanol [53], Z-5-nonadecene [54],…”

Section: Discussionmentioning

confidence: 99%

Antibacterial activity and chemical profile of the bioactive compounds from Aloe polyphylla Schönland

Maliehe

Ngidi

Shandu

et al. 2022

Preprint

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Background The availability of knowledge regarding the ethnomedicinal value and phytochemistry of Aloe polyphylla Schönland has neither been documented nor fully explored. However, this aloe might possess phytocompounds of some medicinal importance. Objective This study aimed to evaluate the antibacterial activity and phytochemical profile of A. polyphylla Schönland gel extract. Methods The gel extract from A. polyphylla Schönland was evaluated for its antibacterial activity and chemical composition using the microdilution method and gas chromatography-mass spectrophotometry (GC-MS), respectively. The molecular interactions of the identified ligands with penicillin‑binding protein 2x (PDP2) were studied by AutoDock Vina to predict the antibacterial mechanisms. Results The extract exhibited antibacterial activity against the selected bacteria with the minimum inhibitory concentration (MIC) values ranging from 0.05 to 0.781 mg/mL. The GC-MS analysis revealed 244 phytoconstituents with 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl (15.66%), 5hydroxymethylfurfural (9.63%), tetradecanoic acid (3.93%), 3-butyl-4-nitro-pent-4-enoic acid, methyl ester (3.51%) and 2',4'-dihydroxy-3'-methylacetophenone (3.26%) as main compounds. The molecular structures of the ligands showed moderate interactions with PBP2, with the docking scores of -5.00, -4.8, -4.3, -5.0 and − 5.1 kcal/mol against 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl, 5-hydroxymethylfurfural, 2',4'-dihydroxy-3'-methylacetophenone, 3-butyl-4-nitro-pent-4-enoic acid, methyl ester and tetradecanoic acid, respectively. Conclusion The gel extract was revealed to have potential pharmacological applicability.

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The GC–MS analysis of the diethylether and ethylacetate fraction of the peel of Solanum incanum and the study of their antibacterial activity

Cited by 5 publications

References 12 publications

Chemical Composition and Antibacterial and Antioxidant Activities of Stem Bark Essential Oil and Extracts of Solanecio gigas

Chemical Composition and Antibacterial and Antioxidant Activities of Stem Bark Essential Oil and Extracts of Solanecio gigas

Kocuria flava, a Bacterial Endophyte of the Marine Macroalga Bryopsis plumosa, Emits 8-Nonenoic Acid Which Inhibits the Aquaculture Pathogen Saprolegnia parasitica

Antibacterial activity and chemical profile of the bioactive compounds from Aloe polyphylla Schönland

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