Chemical Composition, Antioxidative and Anti-Inflammatory Activity of Extracts Prepared from Aerial Parts of <i>Oenothera biennis</i> L. and <i>Oenothera paradoxa</i> Hudziok Obtained after Seeds Cultivation

Granica, Sebastian; Czerwińska, Monika E.; Piwowarski, Jakub P.; Ziaja, Maria; Kiss, Anna K.

doi:10.1021/jf304002h

Cited by 80 publications

(50 citation statements)

References 42 publications

(104 reference statements)

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“…However, taking into account the fact that the total polyphenol content in the ethanolic O. paradoxa extract was 55% and the identified polyphenols comprised 15% (Table 3), we cannot rule out the possibility that 30% of the remaining extract polyphenolics would be procyanidins, particularly since the HPLC-UV analysis of the extract constituents in our study showed the presence of a wide peak characteristic for polymeric proanthocyanidins that reflected their high content (data not shown) [23]. It is noteworthy that catechin was the major component among the identified polyphenols in the O. paradoxa extract, according to previous studies [9, 11, 23, 40]. Catechins are well-known bioactive compounds and potent antioxidants as has been described in in vitro and in vivo studies [4, 41].…”

Section: Discussionmentioning

confidence: 58%

Dual Stimulus-Dependent Effect ofOenothera paradoxaExtract on the Respiratory Burst in Human Leukocytes: Suppressing forEscherichia coliand Phorbol Myristate Acetate and Stimulating for Formyl-Methionyl-Leucyl-Phenylalanine

Burzyńska-Pędziwiatr

Bukowiecka‐Matusiak

Wójcik

et al. 2014

Oxidative Medicine and Cellular Longevity

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Although a growing body of evidence suggests that plant polyphenols can modulate human immune responses, their simultaneous action on monocyte and neutrophil oxidative burst is currently poorly understood. Based on the hypothesis that various polyphenols contained in plant extracts might affect the oxidative burst of phagocytes, we evaluated the effects of ethanolic O. paradoxa extract polyphenols on monocyte and neutrophil oxidative burst in vitro activated by different stimuli, including opsonized bacteria E. coli, phorbol 12-myristate 13-acetate (PMA), and formyl-methionyl-leucyl-phenylalanine (fMLP). Samples were analyzed by the dihydrorhodamine flow cytometry assay. Our results showed that the extract repressed significantly and dose-dependently reactive oxygen species production in both cell types stimulated with E. coli and PMA (P < 0.05) and its inhibitory efficiency was stimulus- and cell-type-dependent. Interestingly, there was significant stimulatory effect of the extract on bursting phagocytes induced by fMLP (P < 0.05). Additionally, several flavonoids and phenolic compounds as well as penta-galloyl-β-(D)-glucose (PGG), the representative of hydrolyzable tannins, were identified in the 60% extract by high-performance liquid chromatography (HPLC) coupled to electrospray ionization in negative ion mode. In summary, the ethanolic O. paradoxa extract, rich in flavonoids and phenolic compounds, exhibits dual stimulus-dependent effect on the respiratory burst in human leukocytes; hence, it might affect immune responses in humans.

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Section: Discussionmentioning

confidence: 58%

Dual Stimulus-Dependent Effect ofOenothera paradoxaExtract on the Respiratory Burst in Human Leukocytes: Suppressing forEscherichia coliand Phorbol Myristate Acetate and Stimulating for Formyl-Methionyl-Leucyl-Phenylalanine

Burzyńska-Pędziwiatr

Bukowiecka‐Matusiak

Wójcik

et al. 2014

Oxidative Medicine and Cellular Longevity

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“…The DPPH radical-scavenging assay was performed according to a previously described method [21]. All tests were performed in triplicate.…”

Section: Methodsmentioning

confidence: 99%

Antidiabetic and Antioxidant Effects and Phytochemicals of Mulberry Fruit (Morus alba L.) Polyphenol Enhanced Extract

et al. 2013

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The antidiabetic and antioxidant activities of the ethyl acetate-soluble extract (MFE) of mulberry fruit (Morus alba L.) were investigated. In vitro, MFE showed potent α-glucosidase inhibitory activity and radical-scavenging activities against DPPH and superoxide anion radicals. In vivo, MFE could significantly decrease fasting blood glucose (FBG) and glycosylated serum protein (GSP), and increase antioxidant enzymatic activities (SOD, CAT, GSH-Px) in streptozotocin (STZ)-induced diabetic mice. Bioactivity-guided fractionation of the MFE led to the isolation of 25 phenolic compounds, and their structures were identified on the basis of MS and NMR data. All the 25 compounds were isolated from mulberry fruit for the first time. Also, the α-glucosidase inhibitory activity and antioxidant activity of the phenolics were evaluated. Potent α-glucosidase inhibitory and radical-scavenging activities of these phenolics suggested that they may be partially responsible for the antidiabetic and antioxidant activities of mulberry fruit.

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“…Compounds were identified basing on their UV-Vis and MS spectra. Comparisons with the available chemical standards and suitable literature were performed [43][44][45][46]. Compounds were quantified using calibration curves for chlorogenic acid at 325 nm for all caffeic acid derivatives found in the extracts or using calibration curve for isoquercitrin for all flavonoids.…”

Section: Uhplc-dad-ms/ms Analysismentioning

confidence: 99%

Phytochemical Profiles and In Vitro Immunomodulatory Activity of Ethanolic Extracts from Galium aparine L.

Ilina

Кашпур

Granica

et al. 2019

Plants

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Galium aparine L., family Rubiaceae, is a widely spread species in the Galium genus. The herb of G. aparine is part of folk remedies and dietary supplements. In this study, we analyzed the chemical composition and immunomodulatory activities of G. aparine herb ethanolic extracts obtained from the plant material by maceration with 20%, 60% or 96% ethanol. The contents of hydroxycinnamic acid derivatives, flavonoids and polyphenols were determined spectrophotometrically, with extractives and polysaccharides quantified gravimetrically. The qualitative composition was studied using UHPLC-DAD-MS/MS analysis; isolation not previously described in G. aparine quercetin rhamnoglucoside was carried out through column chromatography, and the immunomodulatory activity of extracts was determined in the reaction of lymphocyte blast transformation. Major constitutes of extracts were iridoids, i.e., monotropein, 10-desacetylasperulosidic acid and asperulosidic acid; p-hydroxybenzoic acid; hydroxycinnamic acid derivatives, i.e., 3-O-caffeoylquinic, 5-O-caffeoylquinic, 3,4-O-dicaffeoylquinic, 3,5-O-dicaffeoylquinic, 4,5-O-dicaffeoylquinic acids and caffeic acid derivatives; flavonoids, i.e., rutin, quercetin 3-O-rhamnoglucoside-7-O-glucoside, and isorhamnetin 3-O-glucorhamnoside. Significantly, quercetin 3-O-rhamnoglucoside-7-O-glucoside was first isolated and identified in Galium species so far investigated. All G. aparine herb ethanolic extracts stimulate the transformational activity of immunocompetent blood cells, with 96% ethanolic extract being the most active. The data obtained necessitate further research into the mechanisms of immunomodulatory activity of extracts from G. aparine herb.Specific immunostimulants include thymus preparations, interleukins, interferons, biologically active peptides, polysaccharides of certain fungi and therapeutic vaccines, whose effect is explained through their ability to influence the metabolism of cells and body tissues and activate immunocompetent cells.Many plant-derived compounds, like sterols, polysaccharides, alkaloids, flavonoids, lectins and glycoprotein, are used for immunomodulation [3]. For example, among polysaccharides, acidic arabinogalactan and ramnogalacturonan have been shown to manifest immunostimulatory effect in vitro and in vivo [4]. Numerous studies look into the immunomodulative activities of saponins [5][6][7][8].The proven effect of triterpenoid glycosides on the immune system of mammals contributed to the development of a wide range of dietary supplements for the prevention of the immune system disturbances, i.e., human immunity system enhancement [9], and for the prevention and treatment of allergies [10,11].In our previous studies, the immunomodulative effect of the aqueous and ethanolic extracts of Galium verum L. herb was established [12,13]. Among other species of the genus Galium L., one of the most widely spread is Galium aparine L., also called cleavers or goosegrass, which can be found all over Ukraine, Europe, Northern America and certain parts of Asia; i...

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Chemical Composition, Antioxidative and Anti-Inflammatory Activity of Extracts Prepared from Aerial Parts of Oenothera biennis L. and Oenothera paradoxa Hudziok Obtained after Seeds Cultivation

Cited by 80 publications

References 42 publications

Dual Stimulus-Dependent Effect ofOenothera paradoxaExtract on the Respiratory Burst in Human Leukocytes: Suppressing forEscherichia coliand Phorbol Myristate Acetate and Stimulating for Formyl-Methionyl-Leucyl-Phenylalanine

Dual Stimulus-Dependent Effect ofOenothera paradoxaExtract on the Respiratory Burst in Human Leukocytes: Suppressing forEscherichia coliand Phorbol Myristate Acetate and Stimulating for Formyl-Methionyl-Leucyl-Phenylalanine

Antidiabetic and Antioxidant Effects and Phytochemicals of Mulberry Fruit (Morus alba L.) Polyphenol Enhanced Extract

Phytochemical Profiles and In Vitro Immunomodulatory Activity of Ethanolic Extracts from Galium aparine L.

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