Chemical Composition and Toxicity of PM10 and PM0.1 Samples near Open-Pit Mines and Coal Power Stations

Abstract

Particulate matter (PM) <10 μm in size represents an extremely heterogeneous and variable group of objects that can penetrate the human respiratory tract. The present study aimed to isolate samples of coarse and ultrafine PM at some distance from polluting industries (1–1.5 km from the border of open-cast mines). PM was collected from snow samples which allowed the accumulation of a relatively large amount of ultrafine particles (UFPs) (50–60 mg) from five objects: three open-cast mines, coal power plants, and control territories. The chemical composition of PM was examined using absorption spectroscopy, luminescence spectroscopy, high-performance liquid chromatography, X-ray diffraction (XRD), and X-ray fluorescence (XRF) analyses of solid particle material samples. Toxicity was assessed in human MRC-5 lung fibroblasts after 6 h of in vitro exposure to PM samples. The absorption spectra of all the samples contained a wide non-elementary absorption band with a maximum of 270 nm. This band is usually associated with the absorption of dissolved organic matter (DOM). The X-ray fluorescence spectra of all the studied samples showed intense lines of calcium and potassium and less intense lines of silicon, sulfur, chlorine, and titanium. The proliferation of MRC-5 cells that were exposed to PM0.1 samples was significantly (p < 0.01) lower than that of MRC-5 cells exposed to PM10 at the same concentration, except for PM samples obtained from the control point. PM0.1 samples—even those that were collected from control territories—showed increased genotoxicity (micronucleus, ‰) compared to PM10. The study findings suggest that UFPs deserve special attention as a biological agent, distinct from larger PMs.

Genotoxic Effects of Carbon-Rock Pm10 and PM0.1 Isolated From Snow Samples in A549 Cell Culture

“…Альвеолярные эпителиальные клетки карциномы человека А549 использовались для экспонирования образцами ТЧ. Для экспериментов клетки были посажены, собраны и подсчитаны, как описано ранее [6]. Финальные концентрации проб ТЧ составляли 0,25, 0,5 и 1 мг/мл, все варианты эксперимента повторялись 3 раза.…”

“…Выполненный ранее анализ химического состава данных микро-и наночастиц методами абсорбционной спектроскопии позволил установить их преимущественно минеральное происхождение [6]. ТЧ содержат минеральное вещество, кристаллические частицы, элементарный углерод и органическое вещество.…”