Characterization of the Stool Microbiome in Hispanic Preschool Children by Weight Status and Time

Hollister, Emily B.; Foster, Byron A.; Dahdouli, Mahmoud; Ramirez, Jesica; Lai, Zhao

doi:10.1089/chi.2017.0122

Cited by 22 publications

(40 citation statements)

References 48 publications

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“…The abundance of Oscillibacter_sp_1_3 and Bacteroides_massiliensis in offspring from HD-inulin dams was significantly reduced compared to HD dams. Bacteroides_massiliensis was significantly enriched in obese children (76) and in German adults who were unsuccessful with weight loss (77). It is also positively correlated with body mass index (BMI) z-scores (76).…”

Section: Discussionmentioning

confidence: 99%

“…Bacteroides_massiliensis was significantly enriched in obese children (76) and in German adults who were unsuccessful with weight loss (77). It is also positively correlated with body mass index (BMI) z-scores (76). Oscillibacter was strongly associated with the diabetic phenotype (78) and also positively correlated with gut permeability (79).…”

Section: Discussionmentioning

confidence: 99%

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Influence of Maternal Inulin-Type Prebiotic Intervention on Glucose Metabolism and Gut Microbiota in the Offspring of C57BL Mice

et al. 2019

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Scope: Maternal obesity leads to glucose intolerance in the offspring. Changes in the gut microbiota are being increasingly implicated in the pathogenesis of diabetes. We hypothesized that inulin intervention during gestation and lactation improves glucose metabolism disorders in mouse offspring from high-fat diet (HD)-fed dams.Procedures: Female C57BL mice were fed a control diet or HD for 4 weeks before mating. After mating, pregnant mice were randomly divided into three groups through gestation and lactation: control diet (CD) group, HD group, and HD treated with inulin (HD-inulin) group. At weaning, glucose metabolic status was assessed. Gut microbial DNA from offspring cecal contents was isolated and processed for metagenomic shotgun sequencing, and taxonomic and functional profiling were performed.Results: Offspring from dams in the HD-inulin groups demonstrated reduced fasting blood glucose, decreased blood glucose area under the curve during the oral glucose tolerance test, and reduced fasting serum insulin and HOMA-IR compared to offspring from dams in the HD group. Nineteen differentially abundant bacterial species were identified between the HD-inulin and HD groups. The HD-inulin group displayed significantly greater abundances of Bacteroides_acidifaciens, Eubacterium_sp_CAG_786, Clostridium_sp_CAG_343, and Bifidobacterium_breve species and lower abundances of Oscillibacter_sp_1_3, Ruminococcus_gnavus_CAG_126, and Bacteroides_massiliensis species. Differentially abundant bacterial species among the three groups were involved in 38 metabolic pathways, including several glucose and lipid metabolism pathways.Conclusion: Our results show that early inulin intervention in HD-fed mouse dams moderates offspring glucose metabolism and gut dysbiosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Influence of Maternal Inulin-Type Prebiotic Intervention on Glucose Metabolism and Gut Microbiota in the Offspring of C57BL Mice

et al. 2019

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“…A randomized controlled trial involving adults showed that modification of the gut microbiota composition by probiotics could reduce body weight [ 11 , 12 ]. Regarding childhood obesity, most previous studies of the gut microbiota have been cross-sectional in design [ 13 , 14 ]. A recent study in obese children revealed that secreted proteins of the gut microbiota affect the microbial composition [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…One prospective study of children that investigated the microbial changes with weight gain over a four-year period showed that the microbiome-host-diet configuration could be a possible predictor of obesity [ 16 ]. However, research on microbial changes over time after weight reduction interventions has remained scarce [ 13 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Lifestyle modifications result in alterations in the gut microbiota in obese children

Cho

2021

BMC Microbiol

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Background The association between the gut microbiota and pediatric obesity was analyzed in a cross-sectional study. A prospective study of obese children was conducted to assess the gut microbial alterations after a weight change. We collected fecal samples from obese children before and after a 2-month weight reduction program that consisted of individual counseling for nutritional education and physical activity, and we performed 16S rRNA gene amplicon sequencing using an Illumina MiSeq platform. Results Thirty-six participants, aged 7 to 18 years, were classified into the fat loss (n = 17) and the fat gain (n = 19) groups according to the change in total body fat (%) after the intervention. The baseline analysis of the gut microbiota in the preintervention stages showed dysbiotic features of both groups compared with those of normal-weight children. In the fat loss group, significantly decreased proportions of Bacteroidetes phylum, Bacteroidia class, Bacteroidales order, Bacteroidaceae family, and Bacteroides genus, along with increased proportions of Firmicutes phylum, Clostridia class, and Clostridiales order, were observed after intervention. The microbial richness was significantly reduced, without a change in beta diversity in the fat loss group. The fat gain group showed significantly deceased proportions of Firmicutes phylum, Clostridia class, Clostridiales order, Lachnospiraceae family, and Eubacterium hallii group genus, without a change in diversity after the intervention. According to the functional metabolic analysis by the Phylogenetic Investigation of Communities by Reconstruction of Unobserved States 2, the “Nitrate Reduction VI” and “Aspartate Superpathway” pathways were predicted to increase significantly in the fat loss group. The cooccurring networks of genera were constructed and showed the different microbes that drove the changes between the pre- and postintervention stages in the fat loss and fat gain groups. Conclusions This study demonstrated that lifestyle modifications can impact the composition, richness, and predicted functional profiles of the gut microbiota in obese children after weight changes. Trial registration ClinicalTrials.govNCT03812497, registration date January 23, 2019, retrospectively registered.

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“…While the Firmicutes to Bacteroides ratio in the gut may be higher during obesity but reduced in lean people [102][103][104][105], a systematic review of available literature indicates that the differences do not represent a consistent feature for distinguishing lean individuals from obese ones [106]. Interestingly, some human studies showed no changes in the Bacteroidetes/Firmicutes ratio between fecal samples of lean and obese subjects [107][108][109][110]. However, any studies have found associations between gut bacteria and "hunger hormones" (i.e., leptin and ghrelin) [111].…”

Section: Obesity and Type-2 Diabetes Mellitus (T2dm)mentioning

confidence: 99%

Health Impact and Therapeutic Manipulation of the Gut Microbiome

et al. 2020

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Recent advances in microbiome studies have revealed much information about how the gut virome, mycobiome, and gut bacteria influence health and disease. Over the years, many studies have reported associations between the gut microflora under different pathological conditions. However, information about the role of gut metabolites and the mechanisms by which the gut microbiota affect health and disease does not provide enough evidence. Recent advances in next-generation sequencing and metabolomics coupled with large, randomized clinical trials are helping scientists to understand whether gut dysbiosis precedes pathology or gut dysbiosis is secondary to pathology. In this review, we discuss our current knowledge on the impact of gut bacteria, virome, and mycobiome interactions with the host and how they could be manipulated to promote health.

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Characterization of the Stool Microbiome in Hispanic Preschool Children by Weight Status and Time

Abstract: Although differences exist between the gut microbiota of obese and NW children, we detected highly individualized responses of the gut microbiota of obese children over time and following weight loss.

Cited by 22 publications

References 48 publications

Influence of Maternal Inulin-Type Prebiotic Intervention on Glucose Metabolism and Gut Microbiota in the Offspring of C57BL Mice

Influence of Maternal Inulin-Type Prebiotic Intervention on Glucose Metabolism and Gut Microbiota in the Offspring of C57BL Mice

Lifestyle modifications result in alterations in the gut microbiota in obese children

Health Impact and Therapeutic Manipulation of the Gut Microbiome

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