Influence of Maternal Inulin-Type Prebiotic Intervention on Glucose Metabolism and Gut Microbiota in the Offspring of C57BL Mice

Abstract

Scope: Maternal obesity leads to glucose intolerance in the offspring. Changes in the gut microbiota are being increasingly implicated in the pathogenesis of diabetes. We hypothesized that inulin intervention during gestation and lactation improves glucose metabolism disorders in mouse offspring from high-fat diet (HD)-fed dams.Procedures: Female C57BL mice were fed a control diet or HD for 4 weeks before mating. After mating, pregnant mice were randomly divided into three groups through gestation and lactation: control diet (CD) group, HD group, and HD treated with inulin (HD-inulin) group. At weaning, glucose metabolic status was assessed. Gut microbial DNA from offspring cecal contents was isolated and processed for metagenomic shotgun sequencing, and taxonomic and functional profiling were performed.Results: Offspring from dams in the HD-inulin groups demonstrated reduced fasting blood glucose, decreased blood glucose area under the curve during the oral glucose tolerance test, and reduced fasting serum insulin and HOMA-IR compared to offspring from dams in the HD group. Nineteen differentially abundant bacterial species were identified between the HD-inulin and HD groups. The HD-inulin group displayed significantly greater abundances of Bacteroides_acidifaciens, Eubacterium_sp_CAG_786, Clostridium_sp_CAG_343, and Bifidobacterium_breve species and lower abundances of Oscillibacter_sp_1_3, Ruminococcus_gnavus_CAG_126, and Bacteroides_massiliensis species. Differentially abundant bacterial species among the three groups were involved in 38 metabolic pathways, including several glucose and lipid metabolism pathways.Conclusion: Our results show that early inulin intervention in HD-fed mouse dams moderates offspring glucose metabolism and gut dysbiosis.

Activation of ectopic olfactory receptor 544 induces GLP-1 secretion and regulates gut inflammation

“… 41 The induction of B. acidifaciens is of interest, because this strain alters the gut commensal bacteria community, thereby improving lipid and glucose metabolism and modulating the levels of GLP-1 in mice. 18–20 , 42 We found that B. acidifaciens levels were elevated in AzA-fed mice, which suggests that AzA significantly increases the population of marker gut microorganisms for leanness.…”

Dietary Supplementation with Inulin Modulates the Gut Microbiota and Improves Insulin Sensitivity in Prediabetes

“…In addition, a significant bifidogenic effect induced by ITF was observed in obese women [21]. Our previous study also showed that inulin supplement increased the relative abundance of Bifido-bacterium_breve in offspring from high-fat diet-(HFD-) fed dams [22]. Bifidobacterium and Lactobacillus had been reported to be poorly represented in the fecal samples of diabetes patients and positively impact insulin sensitivity [23,24].…”

“…In addition, a significant bifidogenic effect induced by ITF was observed in obese women [ 21 ]. Our previous study also showed that inulin supplement increased the relative abundance of Bifidobacterium _ breve in offspring from high-fat diet- (HFD-) fed dams [ 22 ].…”

The Underlying Mechanisms of Curcumin Inhibition of Hyperglycemia and Hyperlipidemia in Rats Fed a High-Fat Diet Combined With STZ Treatment

“…In the present study, we observed that the area under the blood glucose curve was decreased after treatment with curcumin, which suggested that curcumin increases tolerance to glucose in rats fed a high-fat diet. Some studies suggest that modulation of glucose tolerance are involved in gut macrobiotics [32] and the insulin signaling path [33]. Direct experimental evidence showed that curcumin improves glucose tolerance via stimulation of glucagon-like peptide-1 secretion [34].…”