Characterization of the soluble alkaline phosphatase from hepatopancreas of Squilla mantis L.

Principato, Giovanni; Aisa, Maria Cristina; Talesa, Vincenzo Nicola; Rosi, Gabriella; Giovannini, Elvio

doi:10.1016/0305-0491(85)90464-x

Cited by 58 publications

(37 citation statements)

References 8 publications

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“…The enzymes activities of Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) were assayed by the method of Reitman and Frankel (1957). Seminal plasma Alkaline phosphatase (AlP) activity was measured at 405 nm by the formation of para-nitrophenol from para-nitrophenylphosphate as a substrate (Principato et al, 1985). Blood triglycerides and Total cholesterol were determined according to Fossati and Prencipe (1982) and Richmond (1973).…”

Section: Methodsmentioning

confidence: 99%

Dietary Boron Supplementation and its Impact on Semen Characteristics and Physiological Status of Adult Male Rabbits

Elkomy¹,

El-Hady²,

Elghalid³

2015

Asian J. of Poultry Science

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This study was carried out to assess the effect of Boron supplementation on male rabbit's reproductive performance. A total of 20 V. Line (V.L) adult rabbit bucks were divided into four equal experimental groups (5 bucks for each). Groups 2, 3 and 4 were fed rations supplemented with 17.5, 35 and 70 mg boron/kg feed in the form of boric acid (100, 200 and 400 mg boric acid, respectively) over 8 weeks. The first group was served as a control group. Results obtained by the Boron groups revealed that adding Boron to rabbit bucks rations at any tested doses resulted in a non-significant increase in the ejaculate volume of male rabbit by approximately 25% above the control group. Adding Boron at any dose exhibited a significant increase (p#0.01) in sperm concentration, total sperm output, sperm motility, live sperm and normal sperm compared to the control group and the high Boron dose had the highest effect on previous measurements compared to the low and medium Boron doses. Opposite trend was shown in the reaction time (libido) and seminal plasma fructose concentration which showed a significant decrease in these parameters in the Boron treated groups compared to the control. Plasma total protein, glucose, total lipids and triglycerides concentration were boosted in the Boron groups compared to the control (p#0.01) for glucose and triglycerides measurements. On the contrary, treated male rabbits with Boron at any tested doses resulted in a significant reduction in plasma cholesterol concentration. Blood and seminal plasma AST and ALT enzymes activities showed a tendency to decrease by Boron treatments compared to the control group. Treating bucks by different Boron doses resulted in a significant increase (p#0.01) in Total Antioxidant Capacity (TAC) to reach 110, 128 and 163% of blood plasma and 114, 121 and 122% of seminal plasma compared to the control value. On the other side, adding Boron to bucks rations did not affect the activity of seminal plasma alkaline phosphatase enzyme. Blood testosterone hormone had a tendency to increase in the Boron groups compared to the control group but this increase was not significant. It could be concluded that feeding rabbit bucks on rations containing the studied Boron doses results in a significant improvement in semen quality characteristics and had a positive effect on their physiological status.

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Section: Methodsmentioning

confidence: 99%

Dietary Boron Supplementation and its Impact on Semen Characteristics and Physiological Status of Adult Male Rabbits

Elkomy¹,

El-Hady²,

Elghalid³

2015

Asian J. of Poultry Science

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“…The homogenate was used for the assay of tissue glycogen content, 15) plasma enzymes like alanine aminotransferase (ALT), aspartate aminotransferase (AST), 16) alkaline phosphatase (ALP), 17) and acid phosphatase (ACP). 18) Statistical Analysis --Statistical analysis was performed using SPSS software version 14.0.…”

Section: Methodsmentioning

confidence: 99%

Influence of Costus speciosus (Koen.) Sm. Rhizome Extracts on Biochemical Parameters in Streptozotocin Induced Diabetic Rats

Daisy¹,

Eliza²,

Ignacimuthu³

2008

JOURNAL OF HEALTH SCIENCE

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Diabetes affects about 4% of the global population and management of diabetes without any side effects is still a challenge to the medical system. The present study investigated the possible protective effects of Costus speciosus (Koen.) sm. (C. speciosus) rhizome extracts on biochemical parameters in streptozotocin (STZ)-induced male diabetic Wistar rats. STZ treatment (50 mg/kg, i.p.) caused a hyperglycemic state that led to various physiologic and biochemical alterations. Hexane, ethyl acetate, and methanol crude extracts administered at the dose of 250 mg/kg, 400 mg/kg, and 400 mg/kg, respectively, for 60 days to STZ-induced hypoglycemic and normoglycemic rats. The plasma glucose concentration was significantly (p < 0.05) decreased by all three extracts compared with controls. In addition, oral administration of hexane extract significantly decreased glycosylated hemoglobin (HbA 1c ), serum total cholesterol, and triglyceride levels, urea, uric acid, and creatinine and at the same time markedly increased plasma insulin, tissue glycogen, serum protein, and highdensity lipoprotein (HDL) cholesterol levels. The hexane crude extract also restored the altered plasma enzymes aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and acid phosphatase (ACP) levels to near normal. Glibenclamide used as a reference drug (0.6 mg/kg body weight) also produced a significant reduction in the blood glucose concentration in STZ-induced diabetic rats. In summary, the hexane crude extract was found to be more active in comparision with ethyl ac- * To whom correspondence should be addressed: Department of Biotechnology, Holy Cross College, Trichy-620002, Tamil Nadu, India. Tel.: +91-431-2700637; Fax: +91-431-271331; E-mail: daisylesslie@yahoo.com etate and methanol extracts. Thus this study shows that the C. speciosus hexane extract has antihyperglycemic and hypolipidemic activity, is able to ameliorate the diabetic state, and is probably a source of hypoglycemic compounds.

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“…Plasma, brain, liver and testes lactate dehydrogenase (LDH, EC1.1.1.27) activity was determined by the method of Cabaud and Wroblewski (1958) [17] . Alkaline phosphatase (AlP; EC 3.1.3.1) activity was measured at 405nm by the formation of paranitrophenol from para nitrophenyl phosphate as a substrate [18] . Acid phosphatase (AcP; EC 3.1.3.2) activity was measured using the method of Moss (1984) [19] .…”

Section: Enzyme Assessmentsmentioning

confidence: 99%

Therapeutic Benefits of Garlic against Alloxan-Induced Diabetic in Rats

El-Gamal¹

2017

jmscr

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Characterization of the soluble alkaline phosphatase from hepatopancreas of Squilla mantis L.

Cited by 58 publications

References 8 publications

Dietary Boron Supplementation and its Impact on Semen Characteristics and Physiological Status of Adult Male Rabbits

Dietary Boron Supplementation and its Impact on Semen Characteristics and Physiological Status of Adult Male Rabbits

Influence of Costus speciosus (Koen.) Sm. Rhizome Extracts on Biochemical Parameters in Streptozotocin Induced Diabetic Rats

Therapeutic Benefits of Garlic against Alloxan-Induced Diabetic in Rats

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