Characterization of the secreted cellulases of Trichoderma reesei wild type and mutants during controlled fermentations

Sheir-Neiss, Geraldine; Montenecourt, Bland S.

doi:10.1007/bf00254645

Cited by 98 publications

(38 citation statements)

References 23 publications

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“…Using a combination of genetic and DNA technology approaches, a few strains with increased specific activities have been reported (Montenecourt et al, 1983;Sheir-Neiss and Montenecourt, 1984). A T. reesei strain KY 746 showed a 50% increase in filter paper activity with only a 25% increase in endoglucanase activity over the wild type QM 9414 (Morikawa et al, 1985), while T. reesei RUT-P 37 showed a doubling of specific activity in both filter paper and endoglucanse units compared with the wild type QM 6a (Montenecourt et al, 1983).…”

Section: Genetics and Recombinant Dna Technologymentioning

confidence: 99%

Lignocellulose biotechnology: issues of bioconversion and enzyme production

Howard¹,

Abotsi²,

Rensburg³

et al. 2003

Afr. J. Biotechnol.

661

309

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This review is written from the perspective of scientists working in lignocellulose bioconversion in a developing country and the aim of this review is to remind ourselves and other scientists working in related areas of lignocellulose research of the enormous economic potential of the bioprocessing of residual plant materials generally regarded as "waste", and secondly to highlight some of the modern approaches which potentially could be used to tackle one of the major impediments, namely high enzyme cost, to speed-up the extensive commercialisation of the lignocellulose bioprocessing.

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Section: Genetics and Recombinant Dna Technologymentioning

confidence: 99%

Lignocellulose biotechnology: issues of bioconversion and enzyme production

Howard¹,

Abotsi²,

Rensburg³

et al. 2003

Afr. J. Biotechnol.

661

309

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“…To determine whether the newly discovered endoglucanases are similarly regulated, we examined mRNA levels for each of these gene products by Northern blot. Two different strains were used: QM6a, a wild type isolate of T. reesei, and RL-P37, a strain that has been selected for improved production of cellulolytic enzymes (47). Mycelia from each of these strains were grown in flasks in minimal media containing glucose, crystalline cellulose (avicel), or glycerol as the sole carbon source, or glycerol supplemented with 1 mM sophorose.…”

Section: Identification Of New Genes Encoding Hydrolytic Enzymes-mentioning

confidence: 99%

“…Moreover, the effect of sophorose on expression of numerous genes appears to differ between the two strains examined. RL-P37 was derived from QM6a by mutation and screening for increased cellulase production in the presence of glycerol and subsequently in 2-deoxyglucose (47). Under the fermentation conditions used in this study, cellulase production in RL-P37 was 10-fold higher than in QM6a.…”

mentioning

confidence: 99%

Transcriptional Regulation of Biomass-degrading Enzymes in the Filamentous Fungus Trichoderma reesei

Foreman¹,

Brown

Dankmeyer³

et al. 2003

Journal of Biological Chemistry

426

341

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The filamentous fungus Trichoderma reesei produces and secretes profuse quantities of enzymes that act synergistically to degrade cellulase and related biomass components. We partially sequenced over 5100 random T. reesei cDNA clones. Among the sequences whose predicted gene products had significant similarity to known proteins, 12 were identified that encode previously unknown enzymes that likely function in biomass degradation. Microarrays were used to query the expression levels of each of the sequences under different conditions known to induce cellulolytic enzyme synthesis. Most of the genes encoding known and putative biomass-degrading enzymes were transcriptionally coregulated. Moreover, despite the fact that several of these enzymes are not thought to degrade cellulase directly, they were coordinately overexpressed in a cellulase overproducing strain. A variety of additional sequences whose function could not be ascribed using the limited sequence available displayed analogous behavior and may also play a role in biomass degradation or in the synthesis of biomass-degrading enzymes. Sequences exhibiting additional regulatory patterns were observed that might reflect roles in regulation of cellulase biosynthesis. However, genes whose products are involved in protein processing and secretion were not highly regulated during cellulase induction.

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“…T. reesei is generally a consistently superior producer of enzymes for hydrolysis, however, the difference in activity between T. reesei and the other fungi diminished when tested on more complex assay substrates and growth media. T. reesei RUT-C30, a hypersecretor of cellulases that is resistant to catabolite repression, does not appear to possess any new or altered enzymes than the parental strain QM6a (Ghosh et al, 1982;Sheir-Neiss and Montenecourt, 1984). Thus, although it produces large amounts of cellulase, total activity may be limited by its ability to degrade the complex and heterogeneous structure of plant cell walls.…”

Section: Hydrolytic Activitymentioning

confidence: 99%

An optimized microplate assay system for quantitative evaluation of plant cell wall–degrading enzyme activity of fungal culture extracts

King

Donnelly

Bergstrom

et al. 2008

Biotech & Bioengineering

138

102

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Developing enzyme cocktails for cellulosic biomass hydrolysis complementary to current cellulase systems is a critical step needed for economically viable biofuels production. Recent genomic analysis indicates that some plant pathogenic fungi are likely a largely untapped resource in which to prospect for novel hydrolytic enzymes for biomass conversion. In order to develop high throughput screening assays for enzyme bioprospecting, a standardized microplate assay was developed for rapid analysis of polysaccharide hydrolysis by fungal extracts, incorporating biomass substrates. Fungi were grown for 10 days on cellulose-or switchgrass-containing media to produce enzyme extracts for analysis. Reducing sugar released from filter paper, Avicel, corn stalk, switchgrass, carboxymethylcellulose, and arabinoxylan was quantified using a miniaturized colorimetric assay based on 3,5-dinitrosalicylic acid. Significant interactions were identified among fungal species, growth media composition, assay substrate, and temperature. Within a small sampling of plant pathogenic fungi, some extracts had crude activities comparable to or greater than T. reesei, particularly when assayed at lower temperatures and on biomass substrates. This microplate assay system should prove useful for high-throughput bioprospecting for new sources of novel enzymes for biofuel production.

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Characterization of the secreted cellulases of Trichoderma reesei wild type and mutants during controlled fermentations

Cited by 98 publications

References 23 publications

Lignocellulose biotechnology: issues of bioconversion and enzyme production

Lignocellulose biotechnology: issues of bioconversion and enzyme production

Transcriptional Regulation of Biomass-degrading Enzymes in the Filamentous Fungus Trichoderma reesei

An optimized microplate assay system for quantitative evaluation of plant cell wall–degrading enzyme activity of fungal culture extracts

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