Characterization of the Mediterranean Italian buffaloes melatonin receptor 1A (MTNR1A) gene and its association with reproductive seasonality

Carcangiu, Vincenzo; Mura, Maria Consuelo; Pazzola, Michele; Vacca, Giuseppe Massimo; Paludo, Marta; Marchi, B; Daga, Cinzia; Bua, Stefano; Luridiana, Sebastiano

doi:10.1016/j.theriogenology.2011.02.018

Cited by 33 publications

(32 citation statements)

References 22 publications

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“…On the other hand, MTNR1A is thought to be the main receptor involved in the regulation of seasonal reproductive activities in mammals (11). Moreover, MTNR1A gene polymorphism has been found to be significantly related to seasonal reproduction in sheep (25), goats (13,26), and buffalo (27). Therefore, MTNR1A gene polymorphism can be used to regulate seasonal and nonseasonal reproductive activities in goats.…”

Section: Discussionmentioning

confidence: 99%

Melatonin receptor 1A gene RsaI and inhibin alpha subunit gene HaeII polymorphisms in Honamli and Hair goat breeds reared in Western Mediterranean region of Turkey

Ağaoğlu¹,

Saatcı²,

Akyüz³

et al. 2015

Turk J Vet Anim Sci

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The melatonin receptor 1A (MTNR1A) and inhibin alpha subunit (INHA) genes play a significant role in the reproductive characteristics of animals. Blood samples were collected from 371 goats (Honamli and Hair) reared in Antalya and Burdur. The polymerase chain reaction (PCR) products were digested by RsaI for the MTNR1A gene and HaeII for the INHA gene. Two alleles (A and G) and three genotypes (AA, AG, and GG) were observed for the INHA gene, while two alleles (R and r) and two genotypes (RR and Rr) were observed for the MTNR1A gene. The highest allelic frequency value for G (91.8%) was found in Honamli goat breeds for the INHA gene while the highest value for R (98.1%) was found in Hair goat breeds for the MTNR1A gene. The GG genotype for the INHA gene and the RR genotype for the MTNR1A gene were identified as the most common genotypes of the Honamli and Hair goat breeds. The rr genotype for the MTNR1A gene could not be determined in the breeds. Both Honamli and Hair goat breeds were in Hardy-Weinberg equilibrium for the genes that were studied. In conclusion, this study confirms the existence of genetic polymorphism in the MTNR1A and INHA genes as detected by PCR-RFLP analysis in Honamli and Hair goat breeds.

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Section: Discussionmentioning

confidence: 99%

Melatonin receptor 1A gene RsaI and inhibin alpha subunit gene HaeII polymorphisms in Honamli and Hair goat breeds reared in Western Mediterranean region of Turkey

Ağaoğlu¹,

Saatcı²,

Akyüz³

et al. 2015

Turk J Vet Anim Sci

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“…The MTNR1A gene has been repeatedly proposed as a candidate gene and seems to play a key role in the photoperiodic control of seasonality that is mediated by the circadian changes in melatonin concentrations. Several studies of this gene in different animal species have found relationships with seasonal reproduction activity (Carcangiu et al, 2011b;Pelletier et al, 2000). Two silent mutations in the sheep MTNR1A gene at positions 606 and 612 of exon II have been found to be associated with seasonal reproduction.…”

Section: Introductionmentioning

confidence: 99%

Influence of melatonin receptor 1A gene polymorphisms on seasonal reproduction in Sarda ewes with different body condition scores and ages

Mura

Luridiana

Bodano

et al. 2014

Animal Reproduction Science

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“…An effective approach is establishing the genetic basis on non-seasonal reproduction breed for improving production in sheep. There is a variety of genes affecting non-seasonal reproduction traits in sheep have been successfully identified such as the melatonin 1A receptor (Chu et al 2003;Hernandez et al 2005;Faigl et al 2008;Mateescu et al 2009;Carcangiu et al 2011) and some clock genes that may be involved in control of seasonal breeding (Notter and Cockett, 2005). However, there were also failed to detect any association between these gene and reproductive seasonality in some ewes (Hernandez et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Association between AA-NAT gene polymorphism and reproductive performance in sheep

Ding-ping¹,

Chengjiang²,

Chen³

2012

Electro Journal of Biotech

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Arylalkylamine N-acetyltransferase (AA-NAT) is critical enzyme in Melatonin (MLT) biosynthesis for MLT regulating the animal seasonal breeding. In this study, DNA sequencing methods were applied to detect the polymorphisms of the AA-NAT gene in 179 Chinese sheep belonging to two non-seasonal reproduction breeds and two seasonal reproduction breeds. One mutation at exon 3 (NM_001009461:c.486A > G) was firstly described at the sheep AA-NAT locus. Hence, we described the SmaI PCR-RFLP method for detecting EX3 486A > G mutation, frequencies of the AA-NAT-G allele varied from 0.871 to 0.908 in two non-seasonal reproduction breeds and 0.517 to 0.578 in two seasonal reproduction breeds. The associations of SmaI polymorphism with estrus traits was analyzed in non-seasonal reproduction breeds sheep and seasonal reproduction breeds sheep, the significant statistical results were found between them, the GG genotype frequencies was higher in non-seasonal reproduction breeds (p < 0.001), while, the GA genotype frequencies was higher in seasonal reproduction breeds (p < 0.05). Hence, the EX3 486A > G mutation could facilitate association analysis and serve as a genetic marker for Chinese sheep breeding and genetics.

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Characterization of the Mediterranean Italian buffaloes melatonin receptor 1A (MTNR1A) gene and its association with reproductive seasonality

Cited by 33 publications

References 22 publications

Melatonin receptor 1A gene RsaI and inhibin alpha subunit gene HaeII polymorphisms in Honamli and Hair goat breeds reared in Western Mediterranean region of Turkey

Melatonin receptor 1A gene RsaI and inhibin alpha subunit gene HaeII polymorphisms in Honamli and Hair goat breeds reared in Western Mediterranean region of Turkey

Influence of melatonin receptor 1A gene polymorphisms on seasonal reproduction in Sarda ewes with different body condition scores and ages

Association between AA-NAT gene polymorphism and reproductive performance in sheep

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