Characterization of Stevia rebaudiana Bertoni Accessions Cultived in Southern Brazil

Francisco, Felipe; Pereira, Gabriely Pinto; Machado, Marília Pereira; Κanis, Luiz Alberto; Deschamps, Cícero

doi:10.5539/jas.v10n3p353

Cited by 11 publications

(14 citation statements)

References 24 publications

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“…Yield values obtained in the present work, notwithstanding, are lower than those reported by Francisco et al (2018) for the same genetic material under similar cultivation conditions on a density of 80 000 plants ha -1 , with planting carried out in September 2014, when it was reported a dry leaf yield of 3255.1 kg ha -1 . This reduction in productivity may be associated with a decrease of 48 days in the vegetative period caused by differences in the transplant period, which occurred in November in the present experiment.…”

Section: Discussioncontrasting

confidence: 78%

“…In a previous evaluation of the same accession under similar cultivation conditions (same region and soil conditions) a rebaudioside A/stevioside ratio of 0.95 and productivity of 695 kg ha -1 of steviol glycosides was obtained in the first cut ( Francisco et al 2018). This result is considerably higher than those reported in the present work.…”

Section: Discussionmentioning

confidence: 90%

“…Also according to the recommendation of the manual, 513 175 313 141 172 Chemical Characteristics Accessions 6, 7 and 14, were identified with rebaudioside A/stevioside ratio close to 1, desirable for the industry. The accession 6, selected for use in the present study, yielded a dry mass of leaves of 4008.3 kg ha -1 , in two harvests, with rebaudioside A/ stevioside ratio of 0.95, and yield of 9.7% of Rebaudioside A in the average of the two harvests (Francisco et al 2018).…”

Section: Experimental Location Field Conditions and General Proceduresmentioning

confidence: 99%

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Plant densities and harvesting times on productive and physiological aspects of Stevia rebaudiana Bertoni grown in southern Brazil

Gomes

Moterle

Biasi

et al. 2018

An. Acad. Bras. Ciênc.

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Stevia (Stevia rebaudiana Bertoni) is a species characterized by producing non-caloric substances with high sweetening potential. Among these substances, rebaudioside A and stevioside are produced in greater quantity. Plant density and harvesting time are factors that affects biomass and sweetening compounds yield in this species. The objective of this research was to evaluate the effect of plant densities and harvesting times on the productive and physiological characteristics of stevia in southern Brazil. The experimental design was in randomized blocks, in a split-plot scheme, with 9 treatments comparing the effect of three planting densities (166 667, 83 333 and 33 333 plants ha -1 ) in the plots and three harvesting periods (before, in the beginning and in full flowering) in the subplots. Harvesting at the beginning of flowering promoted higher dry leaf biomass yield and, when associated with the lowest planting density, promoted higher levels of rebaudioside A and stevioside. The lowest planting density resulted in greater leaves biomass accumulation, whereas the highest density promoted higher yields per area. Interaction between density of 166 667 plants ha -1 and the harvest at the onset of flowering promoted higher yields of rebaudioside A (43.22 kg ha -1 ) as well as higher rebaudioside A/stevioside ratio (0.60).

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Section: Discussioncontrasting

confidence: 78%

Section: Discussionmentioning

confidence: 90%

Section: Experimental Location Field Conditions and General Proceduresmentioning

confidence: 99%

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Plant densities and harvesting times on productive and physiological aspects of Stevia rebaudiana Bertoni grown in southern Brazil

Gomes

Moterle

Biasi

et al. 2018

An. Acad. Bras. Ciênc.

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“…Increased transcript levels of the UGT76G1 enzymes increased the level of the final product of the biosynthetic pathway, rebaudioside A 45,46 . Rebaudioside A accumulation is one of the most important traits contributing to the economic value of stevia crops 47 .…”

Section: Discussionmentioning

confidence: 99%

Comparison of uridine diphosphate-glycosyltransferase UGT76G1 genes from some varieties of Stevia rebaudiana Bertoni

Abdelsalam

Botros

Khaled

et al. 2019

Sci Rep

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Stevia leaves contain various components, such as flavonoids, labdanes, chlorophylls, sterols, triterpenoids, mono-disaccharides, organic acids and inorganic salts. Stevia is known to accumulate diterpenoid steviol glycosides, which are approximately 300 times sweeter than regular sugar. Stevioside and rebaudioside A are the main diterpenic glycosides in stevia. Steviol glycosides are the secondary metabolites responsible for the sweetness of stevia. The main objectives of the present study were to determine the concentrations of diterpenic glycosides (stevioside and rebaudioside A) in three stevia varieties ( Stevia rebaudiana) via the HPLC-UV technique and to amplify the UGT76G1 gene by PCR using gene-specific primers. The expression levels of the UGT76G1 gene were determined in the three stevia varieties. The PCR products were sequenced and analyzed, and the nucleotide sequences of the UGT76G1 gene were submitted to GenBank and assigned to the following three varieties: Egy1 (MH087463), China1 (MH087464) and Sponti (MH087465). Cluster analysis was used to separate the three varieties into two major clusters based on their phylogenetic relationship. In addition, chemical analysis was carried out to evaluate stevioside and rebaudioside A. The present study concluded that Egy1 and Sponti are closely related varieties as they fall in the same cluster, while China1 forms a separate cluster. Bioprospecting studies could be useful for selection of superior ecotypes of Stevia rebaudiana .

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“…Main SVglys are stevioside (ST), rebaudioside A, C, D, E, F (Reb A-F), dulcoside A (Dulc A) and steviolbioside. In S. rebaudiana , a wide phenotypic variability in SVglys profiles can be observed 7 – 9 . For example, some genotypes synthetize very high proportions of Reb A and/or Reb C, whereas others accumulate preferentially their corresponding substrates ST and Dulc A, respectively 10 , 11 .…”

Section: Introductionmentioning

confidence: 99%

Development of screening methods for functional characterization of UGTs from Stevia rebaudiana

Petit

Camborde

Vallejo

et al. 2020

Sci Rep

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Glycosylation is a key modification that contributes to determine bioactivity and bioavailability of plant natural products, including that of terpenoids and steviol glycosides (SVglys). It is mediated by uridine-diphosphate glycosyltransferases (UGTs), that achieve their activity by transferring sugars on small molecules. Thus, the diversity of SVglys is due to the number, the position and the nature of glycosylations on the hydroxyl groups in C-13 and C-19 of steviol. Despite the intense sweetener property of SVglys and the numerous studies conducted, the SVglys biosynthetic pathway remains largely unknown. More than 60 SVglys and 68 putative UGTs have been identified in Stevia rebaudiana. This study aims to provide methods to characterize UGTs putatively involved in SVglys biosynthesis. After agroinfiltration-based transient gene expression in Nicotiana benthamiana, functionality of the recombinant UGT can be tested simply and directly in plants expressing it or from a crude extract. The combined use of binary vectors from pGWBs series to produce expression vectors containing the stevia's UGT, enables functionality testing with many substrates as well as other applications for further analysis, including subcellular localization.

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Characterization of Stevia rebaudiana Bertoni Accessions Cultived in Southern Brazil

Cited by 11 publications

References 24 publications

Plant densities and harvesting times on productive and physiological aspects of Stevia rebaudiana Bertoni grown in southern Brazil

Plant densities and harvesting times on productive and physiological aspects of Stevia rebaudiana Bertoni grown in southern Brazil

Comparison of uridine diphosphate-glycosyltransferase UGT76G1 genes from some varieties of Stevia rebaudiana Bertoni

Development of screening methods for functional characterization of UGTs from Stevia rebaudiana

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