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Universidade Federal do Paraná, Curitiba, PR, Brasil. * Autor para correspondência RESUMOVarronia curassavica Jacq. (Boraginaceae), ervabaleeira, é uma planta medicinal com interesse comercial devido sua atividade anti-inflamatória. A espécie pode ser propagada por sementes, todavia a propagação vegetativa possibilita a obtenção de plantios mais uniformes em relação aos aspectos agronômicos e fitoquímicos. Nesse sentido, objetivou-se com o presente trabalho, avaliar o efeito da aplicação de diferentes concentrações de ácido indol butírico (AIB) e a confecção de estacas caulinares com diferentes números de folhas, na indução da rizogênese da espécie. O experimento foi conduzido no mês de agosto (estação de inverno), em arranjo fatorial 3x2, com três concentrações de AIB (0, 1500 e 3000 mg L -1 ) e dois tipos de estacas (estacas confeccionadas com duas folhas ou uma folha). O experimento foi conduzido em casa de vegetação com nebulização intermitente. Após 60 dias avaliouse a porcentagem de estacas enraizadas, número de raízes por estaca, comprimento das três maiores raízes por estaca, porcentagem de estacas com calos, porcentagem de estacas vivas não enraizadas, porcentagem de estacas mortas e porcentagem de estacas com brotação. Houve diferença significativa para número de raízes por estaca, que foi maior na concentração de 3000 mg L -1 em relação ao tratamento sem aplicação de AIB (32,5%), e para porcentagem de estacas com calos (15,0%), também maior para estacas com duas folhas na concentração de 3000 mg L -1 de AIB. Para os demais fatores não foi observada diferença estatística entre os tratamentos. Conclui-se que o número de folhas e diferentes concentrações de AIB tiveram baixa influência no enraizamento adventício da espécie, principalmente devido a estação de inverno. PALAVRAS-CHAVE:Varronia curassavica Jacq., plantas medicinais, propagação vegetativa, auxina. ABSTRACTVarronia curassavica Jacq., black sage, is a medicinal plant with commercial interest due its anti-inflammatory properties. This species can be propagated by seeds, via vegetative propagation enabling the achievement of more uniform crops in relation to the agronomic and phytochemicals aspects. Thus, the present study aimed to evaluate the effect of different concentrations of indole butyric acid (IBA) and different leaf numbers in softwood stem cuttings of black sage plants. The experiment was conducted in august (winter season) in a factorial arrangement 3x2, with three different concentrations of IBA (0, 1500 and 3000 mg L -1 ) and two numbers of leaves (two leaves or one leaf). The experiment was conducted in a greenhouse under intermittent mist conditions. At 60 days after planting, the rooting percentage, number of roots per cutting, length of the three longest roots, percentage of cuttings with callus, percentage of live cuttings without roots nor callus, percentage of dead cuttings and percentage of cuttings with shoots were evaluated. There were significant differences for number of roots per cutting, whi...

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Passiflora actinia Hook (common name: ‘maracujá do mato’) is an important medicinal species due to significant sedative and anxiolytic activities. In order to commercially exploit the plant, however, studies on propagation to improve rooting in stem cuttings are needed. The present study was designed to evaluate the effect of the brown seaweed (Ascophyllum nodosum (L.) Le Jol.) extract when applied on P. actinia stem cuttings bases. Five concentrations of the extract in distilled water were evaluated: 0% (pure distilled water), 10%, 20%, 30% and 40%. The experimental design was completely randomized with 4 repetitions and 12 cuttings per experimental unit. Cuttings were evaluated 45 days from planting. Data were analyzed through polynomial regression analysis and Pearson’s correlation coefficients were calculated for all the variables. On average, 51.27% rooting was achieved. Rooting percentage increased linearly according to the brown seaweed extract concentrations. When compared to the control treatment, about 10% increase in rooting was observed at the treatment with 40% seaweed extract. Leaf retention response to A. nodosum increasing concentrations was also adjusted in a positive linear model. A 15.6% increase in leaf retention was observed at the 40% seaweed concentration when compared to the control. Positive correlations were observed for leaf retention and rooting percentage and leaf retention and roots length. The immersion of P. actinia stem cuttings bases in A. nodosum extract at a concentration of 40% promote positive effects on rooting and facilitate the species propagation.

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Tissue culture technique can be an important approach for the rapid propagation of Humulus lupulus L. (hop plant). The present study evaluated different culture media for hop plants (cv. Columbus) in vitro shoots multiplication, as well as ex vitro rooting and plantlets establishment under field conditions at Southern Brazil. The culture medium containing the plant growth regulator thidiazuron (TDZ) resulted in greater in vitro shoots multiplications (five shoots per explant). For microcuttings ex vitro rooting, indole-3-butyric acid (IBA) application at a concentration of 3000 mg L -1 resulted in higher rooting and survival percentages during acclimatization. After 90 days of acclimatization, the plantlets were transplanted under field conditions and had their development and productivity evaluated 122 days after transplanting. Micropropagated plants achieved 100% survival under field conditions. Nitrogen supply is highly necessary for hop plants initial development at Southern Brazil. Micropropagation is a viable technique for quality plantlet production of H. lupulus cv. Columbus.

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