Comparison of uridine diphosphate-glycosyltransferase UGT76G1 genes from some varieties of Stevia rebaudiana Bertoni

Abstract

Stevia leaves contain various components, such as flavonoids, labdanes, chlorophylls, sterols, triterpenoids, mono-disaccharides, organic acids and inorganic salts. Stevia is known to accumulate diterpenoid steviol glycosides, which are approximately 300 times sweeter than regular sugar. Stevioside and rebaudioside A are the main diterpenic glycosides in stevia. Steviol glycosides are the secondary metabolites responsible for the sweetness of stevia. The main objectives of the present study were to determine the concentrations of diterpenic glycosides (stevioside and rebaudioside A) in three stevia varieties ( Stevia rebaudiana) via the HPLC-UV technique and to amplify the UGT76G1 gene by PCR using gene-specific primers. The expression levels of the UGT76G1 gene were determined in the three stevia varieties. The PCR products were sequenced and analyzed, and the nucleotide sequences of the UGT76G1 gene were submitted to GenBank and assigned to the following three varieties: Egy1 (MH087463), China1 (MH087464) and Sponti (MH087465). Cluster analysis was used to separate the three varieties into two major clusters based on their phylogenetic relationship. In addition, chemical analysis was carried out to evaluate stevioside and rebaudioside A. The present study concluded that Egy1 and Sponti are closely related varieties as they fall in the same cluster, while China1 forms a separate cluster. Bioprospecting studies could be useful for selection of superior ecotypes of Stevia rebaudiana .

Soil Application of Nano Silica on Maize Yield and Its Insecticidal Activity Against Some Stored Insects After the Post-Harvest

“…Elements such as Nitrogen, Phosphor, and Potassium, abbreviated as NPK, are considered vital macronutrients for meristematic production and several physiological processes in plant [6][7][8][9][10][11][12][13][14][15] for instance, shoot, root system, flowers etc., moreover, leading to effective water translocation and nutrition, improve the process of photosynthesis [16]. On the other hand, silicon can be considered as a micro nutrient and it is supportive for plant growth, mainly in dry environments, in order to hold water and bind other nutrients, in addition to increasing the cell strength [17].…”

The use of Unmanned Aerial Vehicles (UAVs) to sample the blow microbiome of small cetaceans

“…possible noise produced by UAV rotors could enhance a disturbance through curiosity, avoidance and stress response in the dolphins [24,25]. Although observing behavioral changes was not the main of our study, during the sampling procedure using UAV, no evident changes in dolphin swimming patterns or anomalous behavior caused by the presence of the drone were noted, possibly related to the distance of the flying vehicle.…”

“…drone reported redirection and behavioral changes related to stress due to the presence of UAVs flying at a height of 10 m for several minutes, while the animals were resting [25]. Possible differences were potentially related to the distance of the drone from the sea surface and to the fact that sampling occurred for a short period of time when animals were feeding or traveling: the length of the duration, the distance of the flying vehicles and the activities of the animal were the reason of no evident avoidance response.…”

Enhanced Heterologous Production of Glycosyltransferase UGT76G1 by Co-Expression of Endogenous prpD and malK in Escherichia coli and Its Transglycosylation Application in Production of Rebaudioside

“…Nowadays, more and more countries are facing a serious public health burden from the consumption of high-calorie sugars such as glucose, fructose, and sucrose [1][2][3]. Diets high in such high-calorie sugar are recognized to cause a serious health problem such as obesity, diabetes, hypertension and cardiac blockage [4,5]. Some countries have levied sugar taxes to reduce sugar consumption [1].…”