Characterization of N2O-producing Xanthomonas-like isolates from biofilters as Stenotrophomonas nitritireducens sp. nov., Luteimonas mephitis gen. nov., sp. nov. and Pseudoxanthomonas broegbernensis gen. nov., sp. nov.

Finkmann, W; Altendorf, Karlheinz; Stackebrandt, Erko; Lipski, André

doi:10.1099/00207713-50-1-273

Cited by 267 publications

(195 citation statements)

References 39 publications

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“…;Gerner-Smidt et al, 1995). S. maltophilia DSM 50170 T (l ATCC 13637 T , a type strain isolated from the pleural fluid of a patient with oral carcinoma ; Hugh & Ryschenko, 1961), S. nitritireducens DSM 12575 T (a type strain isolated from an ammonia-supplied biofilter ; Finkmann et al, 2000) and Xanthomonas campestris DSM 3586 T were used as reference strains. All S. maltophilia isolates were identified using the API system (BioMe!…”

Section: Methodsmentioning

confidence: 99%

“…Identification of the fatty acid methyl esters was performed by GC-MS as described by McInroy & Kloepper (1995). Data on the fatty acids from S. nitritireducens strains were analysed by Finkmann et al (2000).…”

Section: Methodsmentioning

confidence: 99%

“…Genotypic analysis, however, revealed only 35 % DNA homology between the two species. Finkmann et al (2000) characterized Stenotrophomonas strains with an unusual denitrification reaction as new species Stenotrophomonas nitritireducens.…”

Section: Abbreviation : Dgge Denaturing-gradient Gel Electrophoresismentioning

confidence: 99%

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Stenotrophomonas rhizophila sp. nov., a novel plant-associated bacterium with antifungal properties.

Wolf¹,

Fritze²,

Hagemann³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

100

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A polyphasic taxonomic study was performed on 16 Stenotrophomonas strains from environmental and clinical sources. A group of three plant-associated isolates were shown to be phenotypically different from the other strains. This group formed a separate physiological cluster (B1) with 42 % heterogeneity to the other isolates. The defining characteristics of the new species were as follows : growth at 4 mC and the absence of growth at 40 mC ; the utilization of xylose as a carbon source ; lower osmolytic tolerance ( 4 5 % NaCl, w/v), although the isolates can produce trehalose and glucosylglycerol as osmoprotective substances ; the absence of lipase and β-glucosidase production ; and antifungal activity against plant-pathogenic fungi. The wholecell fatty acid profile of this group was different and characterized by the main fatty acids iso-C 15 :0 and anteiso-C 15 :0 . Numerical analysis of the fatty acid profiles of the strains examined supports the differentiation of the physiological B1 group. By 16S rDNA analysis, three clusters were distinguished. The three strains of the B1 group formed a separate environmental cluster (E1). They showed a mean similarity of 99 5 % within the cluster, and differed from strains of a second environmental cluster (E2) by 2 2 % and from the clinical cluster (C) by about 3 0 %. DNA-DNA hybridization data supported the taxonomic differentiation. All results led to the proposal of a new species, Stenotrophomonas rhizophila sp. nov., with strain e-p10 T

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Stenotrophomonas rhizophila sp. nov., a novel plant-associated bacterium with antifungal properties.

Wolf¹,

Fritze²,

Hagemann³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

100

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“…The fatty acid profile of strain RIB1-20 T (Table 1) was similar to those given for L. composti and L. mephitis (Young et al, 2007;Finkmann et al, 2000), but there were some significant differences (Table 1).…”

mentioning

confidence: 58%

Luteimonas aquatica sp. nov., isolated from fresh water from Southern Taiwan

Chou

Cho

Arun

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A yellow-pigmented bacterial strain, designated RIB1-20 T , isolated from fresh water was investigated by means of a polyphasic taxonomic approach. The cells were Gram-negative, rodshaped and non-spore-forming. Phylogenetic analyses with the 16S rRNA gene sequence showed that the strain formed a monophyletic branch towards the periphery of the evolutionary radiation occupied by the genus Luteimonas, its two closest neighbours being Luteimonas composti CC-YY255 T (96.1 % sequence similarity) and Luteimonas mephitis B1953/27.1 T (95.8 %). Strain RIB1-20 T was clearly distinguished from both of those type strains using phylogenetic analysis, DNA-DNA hybridization, fatty acid composition data and a range of physiological and biochemical characteristics. It is evident from the genotypic and phenotypic data that strain RIB1-20 T represents a novel species of the genus Luteimonas, for which the name Luteimonas aquatica sp. nov. is proposed. The type strain is RIB1-20 T (5BCRC 17731 T 5LMG 24212 T ).During the characterization of micro-organisms in water samples collected from a freshwater spring located in Kaohsiung County, Taiwan, a yellow-coloured strain, designated RIB1-20 T , was isolated and maintained on R2A agar (BD Difco) plates after incubation at 25 u C for 3 days. Subcultivation was performed on R2A agar at 25 uC for between 48 and 72 h. On this medium, strain RIBI-20 T was able to grow at 15-37 u C, but not at 10 or 40 u C. The organism was able to grow on R2A, nutrient agar (BD Difco) and tryptic soy agar (BD Difco).Cells were observed with phase-contrast microscopy (DM 2000; Leica) in the lag, exponential and stationary phases of growth to ascertain their morphology. Motility was tested by means of the hanging-drop method. The Gram stain set S kit (BD Difco) and the Ryu non-staining KOH method (Powers, 1995) were used to test the Gram-staining reaction. Accumulation of poly-b-hydroxybutyrate granules was investigated using light microscopy after staining of the cells with Sudan black. Colony morphology was observed on R2A agar, using a stereoscopic microscope (SMZ 800; Nikon). Details of the morphology are given in the species description.The pH range for growth was determined by measuring the OD 600 of cultures grown on nutrient broth (BD Difco) adjusted to various pH values (pH 4-10, in increments of 1.0 pH unit), prior to sterilization, using appropriate biological buffers (Chung et al., 1995). To investigate NaCl tolerance, nutrient broth was prepared according to the formula of the BD Difco medium, while NaCl concentrations were varied (0 , 0.5 and 1.0-10.0 %, w/v, in increments of 1.0 %). Growth under anaerobic conditions was determined after incubating strain RIB1-20 T in an Oxoid AnaeroGen system. Strain RIB1-20 T was examined for a broad range of phenotypic properties. Catalase, oxidase, DNase, arginine dihydrolase, urease and lipase activities and hydrolysis of starch, casein and Tweens 20, 40, 60 and 80 were determined using standard methods (Gerhardt et al., 1994; Lányí, 1987;MacFaddin, 2000)....

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“…The genus was characterized as having Gram-stainnegative, oxidase-and catalase-positive rod-shaped cells with a polar flagellum and containing iso-C 15 : 0 , iso-C 17 : 1 v9c and iso-C 17 : 0 as the major fatty acids (Mergaert et al, 2002) and phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), aminolipid (AL), aminophospholipid (APL) and phospholipid (PL) as the main polar lipids (Kämpfer et al, 2010). The quinone system of the members of the genus Fulvimonas, which is a member of the family Xanthomonadaceae, was not reported, however, the main quinone system found in other members of the family Xanthomonadaceae is ubiquinone Q-8 (Finkmann et al, 2000;Kämpfer et al, 2010).…”

mentioning

confidence: 99%

Fulvimonas yonginensis sp. nov., isolated from greenhouse soil, and emended description of the genus Fulvimonas

Ahn

Kim

Weon

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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Fulvimonas yonginensis sp. nov., isolated from greenhouse soil, and emended description of the genus Fulvimonas Jae-Hyung Ahn, Soo-Jin Kim, Hang-Yeon Weon, Seung-Beom Hong, Soon-Ja Seok and Soon-Wo Kwon Correspondence Soon-Wo Kwon swkwon1203@korea.kr Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea A novel strain, designated 5GHs31-2 T , was isolated from a greenhouse soil sample collected from Yongin city, South Korea. Cells of strain 5GHs31-2 T were Gram-stain-negative, rod-shaped, polar flagellated and yellow-pigmented. The isolate was aerobic, catalase-negative and oxidasepositive and grew optimally at 28-30 6C and pH 7.0. Strain 5GHs31-2 T revealed the highest 16S rRNA gene sequence similarities with Fulvimonas soli LMG 19981 T (97.6 %), Dyella thiooxydans ATSB10 T (97.5 %) and Frateuria terrea VA24 T (97.4 %). Furthermore, the neighbourjoining tree showed that strain 5GHs31-2 T was a representative of a member of the genus Fulvimonas. Strain 5GHs31-2 T contained iso-C 16 : 0 , summed feature 9 (iso-C 17 : 1 v9c and/or C 16 : 0 10-methyl), iso-C 15 : 0 and iso-C 17 : 0 as the major fatty acids, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an aminolipid as the main polar lipids and Q-8 as the predominant quinone. The genomic DNA G+C content of 5GHs31-2 T was 73.0 mol%. According to DNA-DNA hybridization experiments, DNA-DNA relatedness values between strain 5GHs31-2 T and its closest phylogenetic neighbours were below 70 %. Based on the taxonomic data, strain 5GHs31-2 T represents a novel species of the genus Fulvimonas, for which the name Fulvimonas yonginensis sp. nov. is proposed. The type strain is 5GHs31-2 T (5KACC 16952 T 5DSM 28344 T ).

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Characterization of N2O-producing Xanthomonas-like isolates from biofilters as Stenotrophomonas nitritireducens sp. nov., Luteimonas mephitis gen. nov., sp. nov. and Pseudoxanthomonas broegbernensis gen. nov., sp. nov.

Cited by 267 publications

References 39 publications

Stenotrophomonas rhizophila sp. nov., a novel plant-associated bacterium with antifungal properties.

Stenotrophomonas rhizophila sp. nov., a novel plant-associated bacterium with antifungal properties.

Luteimonas aquatica sp. nov., isolated from fresh water from Southern Taiwan

Fulvimonas yonginensis sp. nov., isolated from greenhouse soil, and emended description of the genus Fulvimonas

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