Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in Nigeria

Kasap, Murat; Fashae, Kayode; Torol, Sinem; Kolayli, Fetiye; Budak, Faik; Vahaboğlu, Haluk

doi:10.1186/1476-0711-9-1

Cited by 39 publications

(34 citation statements)

References 23 publications

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“…In recent years, ESBL production among these isolates is becoming a major clinical concern because of its ability to develop resistance to several classes of antimicrobial agents and has high potential for transmission of resistance to other bacterial species (16). In the current study, the most prevalent clinical Enterobacter species was E. cloacae (78.2 %) that showed the important role of this species in human infections, which was in agreement with other reports (17)(18)(19)(20).…”

Section: Discussionsupporting

confidence: 82%

Emergence of blaCTX-M-15 Gene and Its Transferability in Enterobacter spp. Isolated From the Hospitals of Tehran, Iran

Rizi¹,

Peerayeh²,

Bakhshi³

et al. 2015

Avicenna J Clin Microbiol Infect

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Background: Enterobacter spp. is increasingly recognized as an important nosocomial pathogen and implicated in many episodes of hospital acquired infections. Objectives: The current study aimed to describe distribution and transferability of bla CTX-M-15 gene, and the antibiotic susceptibility pattern in the clinical isolates of Enterobacter spp. Materials and Methods: A total of 110 Enterobacter isolates were collected from five hospitals in Tehran, Iran from May 2012 to April 2013. Enterobacter species were identified by API 20E system. Antibacterial susceptibility was determined by disk diffusion method, and extended spectrum beta lactamase (ESBL) production was confirmed by combined disk method. The bla CTX-M-15 gene was identified by PCR with specific primers. The transferability of the bla CTX-M-15 was tested by conjugation with broth matting method. Results: The prevalence of Enterobacter species was E. cloacae (78.2 %), E. aerogenes (6.13 %) and E. sakazakii (8.2%). They were from different clinical sources. Maximal resistance in Enterobacter isolates was noticed against Augmentin®, trimethoprim -sulfamethoxazole and cefoxitin 75.5%, 64.5%, and 59.1%, respectively. Fourteen isolates, showed ESBL phenotype. The bla CTX-M-15 gene frequency in Enterobacter isolates was 11.8%. Three conjugative plasmids containing bla CTX-M-15 were found in one Enterobacter isolate.

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Section: Discussionsupporting

confidence: 82%

Emergence of blaCTX-M-15 Gene and Its Transferability in Enterobacter spp. Isolated From the Hospitals of Tehran, Iran

Rizi¹,

Peerayeh²,

Bakhshi³

et al. 2015

Avicenna J Clin Microbiol Infect

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“…The majority of these enzymes are derived by amino acid substitutions from the narrow spectrum precursors, TEM-1,2 and SHV-15 [2]. SHV-type ESBLs, encoded by bla SHV genes, confer resistance to a broad spectrum of b-lactam antimicrobial agents and are of significant therapeutic concern for infections caused by many species of gram-negative bacteria [3,4].…”

mentioning

confidence: 99%

Emergence of SHV-12 extended spectrum beta-lactamase among clinical isolates of Enterobacter cloacae in Tunisia

Lahlaoui¹,

Anis²,

Kheder³

et al. 2012

Microbial Pathogenesis

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“…This SHV-12 type extended-spectrum β-lactamase is most often found in Asia (Kim et al, 1998) and including Africa (Kasap et al, 2010). It is already found in Algeria, as shown previously in E. cloacae , and K. pneumonia (Berrazeg et al, 2013).…”

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confidence: 53%

Prevalence and molecular typing of extended-spectrum -lactamases in Escherichia coli, Enterobacter cloacae and Citrobacter freundii isolates from Laghouat Hospital, Algeria

Lagha¹,

Hassaïne²,

Robin³

et al. 2016

Afr. J. Microbiol. Res.

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The antibiotic resistance of enterobacteriacae knows a worldwide worrying evolution with an increase of the extended-spectrum β-lactamases. The present study was to determine the prevalence and molecular typing of extended-spectrum β-lactamases (ESBLs) in clinical isolates of Escherichia coli, Enterobacter cloacae, and Citrobacter freundii, isolated between January 2010 and December 2012, at the Laghouat "Ahemida Ben Adjila" hospital, Algeria. Antimicrobial susceptibility testing was determined by disk diffusion on Mueller Hinton agar. Genetic transfers were performed by conjugation and plasmid DNA was extracted by the alcalin-lysis method. The characterization of ESBL genes were examined using PCR amplification and DNA sequencing and the clonal relatedness was investigated by ERIC-PCR. During the study period, twenty-one (8.23%) isolates were found to produce ESBLs, distributed as follows: 13 isolates of E. coli (61.9%), 6 isolates of E. cloacae (28.57%) and 2 isolates of C. freundii with 9.52 %. The CTX-M-15 ESBL were predominant (95.24%), followed by TEM-4 (14.28%) and SHV-12 (4.76%). ERIC-PCR analysis showed that the isolates are genetically unrelated and conjugation experiments showed that bla CTX-M-15 gene was transferred on a conjugative plasmid of high molecular weight (≈130 kb). This study indicated a high prevalence of CTX-M-15 enzymes among E. coli, E. cloacae and C. freundii in Laghouat hospital, Algeria.

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Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in Nigeria

Cited by 39 publications

References 23 publications

Emergence of blaCTX-M-15 Gene and Its Transferability in Enterobacter spp. Isolated From the Hospitals of Tehran, Iran

Emergence of blaCTX-M-15 Gene and Its Transferability in Enterobacter spp. Isolated From the Hospitals of Tehran, Iran

Emergence of SHV-12 extended spectrum beta-lactamase among clinical isolates of Enterobacter cloacae in Tunisia

Prevalence and molecular typing of extended-spectrum -lactamases in Escherichia coli, Enterobacter cloacae and Citrobacter freundii isolates from Laghouat Hospital, Algeria

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