Emergence of SHV-12 extended spectrum beta-lactamase among clinical isolates of Enterobacter cloacae in Tunisia

Lahlaoui, Hella; Anis, Ben Haj Khalifa; Kheder, Mohamed; Mohamed, Ben Moussa

doi:10.1016/j.micpath.2012.04.003

Cited by 13 publications

(12 citation statements)

References 16 publications

(12 reference statements)

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“…Among the 87 isolates, about twothirds produced ESBLs. Several studies have also shown the emerging problem of ESBL-producing E. coli and K. pneumoniae isolates in different geographic regions, including the Mediterranean basin [19] and North Africa [9,[20][21][22][23]. In our work, blaCTX-M-15 was the most frequently detected (54%) gene in the ESBLpositive Tunisian and Libyan isolates.…”

Section: Discussionsupporting

confidence: 56%

Carbapenemases and extended-spectrum β-lactamases producing Enterobacteriaceae isolated from Tunisian and Libyan hospitals

Mathlouthi

Al-Bayssari

Salabi

et al. 2016

J Infect Dev Ctries

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Introduction: The aim of the study was to investigate the prevalence of extended-spectrum β-lactamase (ESBL) and carbapenemase production among clinical isolates of Enterobacteriaceae recovered from Tunisian and Libyan hospitals. Methodology: Bacterial isolates were recovered from patients in intensive care units and identified by biochemical tests and MALDI-TOF. Antibiotic susceptibility testing was performed by disk diffusion and the E-test method. ESBL and carbapenemase activities were detected using standard microbiological tests. Antibiotic resistance-encoding genes were screened by PCR and sequencing. Clonal relationships between Klebsiella pneumoniae strains were carried out using multi-locus sequence typing (MLST). Results: A total of 87 isolates were characterized, with 51 and 36, respectively, identified as E. coli and K. pneumoniae. Overall the resistance prevalence was high for aminoglycosides (> 60%), fluoroquinolones (> 80%), and extended-spectrum cephalosporins (> 94%), and was low for imipenem (11.4%). Among this collection, 58 strains (66.6%) were ESBL producers and 10 K. pneumoniae strains (11.4%) were carbapenemase producers. The antibiotic resistance-encoding genes detected were blaCTX-M-15 (51.7%), blaTEM-1 (35.6%), several variants of blaSHV (21.8%), and blaOXA-48 (11.4%). The MLST typing of K. pneumoniae isolates revealed the presence of multiple clones and three novel sequence types. Also, close relationships between the OXA-48-producing strains from Tunisia and Libya were demonstrated. Conclusions: This study is the first paper describing the emergence of carbapenemase-and ESBL-producing Enterobacteriaceae, sensitive to colistin, isolated in Tunisia and Libya. Active surveillance and testing for susceptibility to colistin should be implementing because resistance to colistin, mainly in Klebsiella, has been recently reported worldwide.

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Section: Discussionsupporting

confidence: 56%

Carbapenemases and extended-spectrum β-lactamases producing Enterobacteriaceae isolated from Tunisian and Libyan hospitals

Mathlouthi

Al-Bayssari

Salabi

et al. 2016

J Infect Dev Ctries

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“…In agreement to this, two isolates producing only SHV-12, although remained susceptible to both CTX and CAZ, had elevated MICs for CTX. In addition, high level of resistance to TIC and ATM observed in these strains may also be related to the SHV-12 production (Lahlaoui et al, 2012;Poirel et al, 2012). Among all ESBL-producing strains, A. caviae isolate (No.11) coproducing CTX-M-15 and PER-1 had the highest MICs for CTX and CAZ Fig.…”

Section: Discussionmentioning

confidence: 92%

Aeromonas spp. simultaneously harbouring blaCTX-M-15, blaSHV-12, blaPER-1 and blaFOX-2, in wild-growing Mediterranean mussel (Mytilus galloprovincialis) from Adriatic Sea, Croatia

Maravić

Skočibušić

Šamanić

et al. 2013

International Journal of Food Microbiology

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“…1). This percentage is higher than that reported in France (16) and Taiwan (9), but lower than that in Tunisia (17). A total of 34.48% (20 out of 58 isolates) of the SHV-12 ESBL genes were located in the bacterial chromosome, 48.28% (28 out of 58 isolates) in plasmids and 17.24% (10 out of 58 isolates) in both (Tables I and II).…”

Section: Resultsmentioning

confidence: 52%

“…Antibiotic resistance and SHV-12 ESBL. (7,17,25). In this study, type C E. cloacae carrying no SHV-12 ESBL were also found to spread between different clinical departments, implying that other factors could also contribute to the spreading of antibiotic-resistant strains in hospital.…”

Section: Shv-12 Esbl Gene Location Affects Antibiotic Resistancementioning

confidence: 99%

Association of antibiotic resistance with SHV-12 extended-spectrum β-lactamase in Enterobacter cloacae

Liu

Lin

et al. 2015

Experimental and Therapeutic Medicine

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Abstract.The association between antibiotic resistance and SHV-12 extended-spectrum β-lactamase (ESBL) in Enterobacter cloacae remains unknown. The aim of the present study was to investigate the prevalence of both chromosome-and plasmid-borne SHV-12 ESBL genes in Enterobacter cloacae. Transmission of the SHV-12 ESBL gene was explored, and the risk factors for antibiotic resistance in E. cloacae were analyzed. Polymerase chain reaction (PCR) results showed that 58 out of the 100 isolates carried the SHV-12 ESBL gene: 34.48% of them occurred in the chromosome, 48.28% were plasmid-borne and 17.24% appeared in both. Enterobacterial repetitive intergenic consensus-PCR tests detected 82 chromosomal genotypes. Conjugation assays showed that 70.00% of plasmid-borne SHV-12 ESBL genes were successfully transconjugated into E. coli C600 and that the antibiotic resistance phenotype of E. cloacae was partially (84%) or completely (10%) transferred. A significantly higher SHV-12 ESBL detection rate was found in patients with underlying conditions and/or complications compared with those without (P<0.05). The detection of SHV-12 ESBL-producing E. cloacae from vertical transmission varied significantly across clinical departments and age groups (P<0.05), with the highest rates in the intensive care unit and the group of patients aged ≥60 years. The present results indicate that the location and transmission efficiency of SHV-12 ESBL are closely correlated with the antibiotic resistance of E. cloacae.

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Emergence of SHV-12 extended spectrum beta-lactamase among clinical isolates of Enterobacter cloacae in Tunisia

Cited by 13 publications

References 16 publications

Carbapenemases and extended-spectrum β-lactamases producing Enterobacteriaceae isolated from Tunisian and Libyan hospitals

Carbapenemases and extended-spectrum β-lactamases producing Enterobacteriaceae isolated from Tunisian and Libyan hospitals

Aeromonas spp. simultaneously harbouring blaCTX-M-15, blaSHV-12, blaPER-1 and blaFOX-2, in wild-growing Mediterranean mussel (Mytilus galloprovincialis) from Adriatic Sea, Croatia

Association of antibiotic resistance with SHV-12 extended-spectrum β-lactamase in Enterobacter cloacae

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