Association of antibiotic resistance with SHV-12 extended-spectrum β-lactamase in Enterobacter cloacae

Abstract

Abstract.The association between antibiotic resistance and SHV-12 extended-spectrum β-lactamase (ESBL) in Enterobacter cloacae remains unknown. The aim of the present study was to investigate the prevalence of both chromosome-and plasmid-borne SHV-12 ESBL genes in Enterobacter cloacae. Transmission of the SHV-12 ESBL gene was explored, and the risk factors for antibiotic resistance in E. cloacae were analyzed. Polymerase chain reaction (PCR) results showed that 58 out of the 100 isolates carried the SHV-12 ESBL gene: 34.48% of them occurred in the chromosome, 48.28% were plasmid-borne and 17.24% appeared in both. Enterobacterial repetitive intergenic consensus-PCR tests detected 82 chromosomal genotypes. Conjugation assays showed that 70.00% of plasmid-borne SHV-12 ESBL genes were successfully transconjugated into E. coli C600 and that the antibiotic resistance phenotype of E. cloacae was partially (84%) or completely (10%) transferred. A significantly higher SHV-12 ESBL detection rate was found in patients with underlying conditions and/or complications compared with those without (P<0.05). The detection of SHV-12 ESBL-producing E. cloacae from vertical transmission varied significantly across clinical departments and age groups (P<0.05), with the highest rates in the intensive care unit and the group of patients aged ≥60 years. The present results indicate that the location and transmission efficiency of SHV-12 ESBL are closely correlated with the antibiotic resistance of E. cloacae.

Antimicrobial susceptibility and molecular epidemiology of clinical Enterobacter cloacae bloodstream isolates in Shanghai, China

“…cloacae was frequently implicated in nosocomial infections[ 1 , 2 ], and the production of pAmpC, ESBLs and carbapenemases have also led to the multidrug-resistance and high potential dissemination of clinical E . cloacae isolates[ 23 , 24 ]. Reports on MDR E .…”

“…Unlike CTX-M-producers most frequent in Latin American countries[ 13 ], SHV was the dominant ESBL type with all identical to type 12 in this study ( Table 2 ). The proportion of SHV (75.0%) was higher than that in Guangdong China (58%) [ 23 ], and similar to that in Tunisia (77.8%) [ 24 ]. SHV-12, one of the most prevalent SHV types in Asia, can confer resistance against β-lactams, fluoroquinolones, aminoglycosides and sulfonamides, and its location and transmission efficiency were correlated closely with the antibiotic resistance of E .…”

“…SHV-12, one of the most prevalent SHV types in Asia, can confer resistance against β-lactams, fluoroquinolones, aminoglycosides and sulfonamides, and its location and transmission efficiency were correlated closely with the antibiotic resistance of E . cloacae [ 23 ]. What’s worse, the coexistence of carbapenemase and/or pAmpC were detected among over half of the SHV-12-producers (4/6) in our study ( Table 3 ), warranting long-term monitoring on phylogenetic screening and prudent use of antibiotics.…”

Critical threat associated with carbapenem-resistant gram-negative bacteria: prioritizing water matrices in addressing total antibiotic resistance

“…The ESBL which is ubiquitous and rampant among Enterobacteriaceae globally has been described by Liakopoulos et al (2016) as neglected yet ubiquitous. It is vital for resistance (Liu et al 2016). Liu et al (2016) showed that the location and transmission efficiency of SHV-12 ESBL are directly linked with the antibiotic resistance of Enterobacter cloacae.…”

“…It is vital for resistance (Liu et al 2016). Liu et al (2016) showed that the location and transmission efficiency of SHV-12 ESBL are directly linked with the antibiotic resistance of Enterobacter cloacae. Conjugation assays conducted by Liu et al (2016) which were transconjugated into E. coli C600 showed 84% partial expression and 10% complete expression of resistance from E. cloacae by plasmid-borne SHV-12 ESBL genes.…”