Characterization of Cucumber mosaic virus infecting snake gourd and bottle gourd in India

Nagendran, K.; Priyanka, R.; Aravintharaj, R.; Balaji, C. G.; Swamy, Prashant; Basavaraj, B.; Mohankumar, S.; Karthikeyan, G.

doi:10.1016/j.pmpp.2018.05.010

Cited by 14 publications

(11 citation statements)

References 16 publications

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“…For tospovirus, the reaction mixture contains total RNA, random primer and nuclease free water up to 12.5 µl were incubated at 65 °C for 5 min followed by 5× reaction buffer, 10 mM dNTP mix, RNase inhibitor and reverse transcriptase were added and incubated for 1 h at 42 °C 47 . The presence of CMV was confirmed through RT-PCR with CMV coat protein gene specific primer (RsCMV-F and RsCMV-R) with a cyclic condition of 94 °C for 2 min followed by 94 °C for 30 s of denaturation, 59 °C for 30 s of annealing, 72 °C for 60 s with final extension of 72 °C for 10 min 48 . The tospovirus was confirmed through RT-PCR using tospovirus universal primer (gL3637 and gL4435C) corresponding to L segment (RdRp) with the cycling condition of 94 °C for 5 min followed by 35 cycles of 94 °C for 30 s, 56 °C for 1 min and 72 °C for 1 min with 72 °C for 10 min of final extension 44 , 49 .…”

Section: Methodsmentioning

confidence: 99%

Co-existence of chlorosis inducing strain of Cucumber mosaic virus with tospoviruses on hot pepper (Capsicum annuum) in India

Vinodhini

Rajendran

Abirami

et al. 2021

Sci Rep

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Cucumo- and tospoviruses are the most destructive viruses infecting hot pepper (chilli). A diagnostic survey was conducted to assess the prevalence of cucumo and tospoviruses in chilli growing tracts of Tamil Nadu. Infected plants showing mosaic with chlorotic and necrotic rings, veinal necrosis, mosaic mottling, leaf filiformity and malformation were collected. Molecular indexing carried out through reverse transcription polymerase chain reaction (RT-PCR) with coat protein gene specific primer of Cucumber mosaic virus (CMV) and tospovirus degenerate primer corresponding to the L segment (RdRp). Ostensibly, amplifications were observed for both CMV and tospoviruses as sole as well for mixed infections. The sequence analysis indicated that the Capsicum chlorosis virus (CaCV) and Groundnut bud necrosis virus (GBNV) to be involved with CMV in causing combined infections. The co-infection of CMV with CaCV was detected in 10.41% of the symptomatic plant samples and combined infection of CMV with GBNV was recorded in around 6.25% of the symptomatic plants surveyed. The amino acid substitution of Ser129 over conserved Pro129 in coat protein of CMV implies that CMV strain involved in mixed infection as chlorosis inducing strain. Further, the electron microscopy of symptomatic plant samples explicated the presence of isometric particles of CMV and quasi spherical particles of tospoviruses. This is the first molecular evidence for the natural co-existence of chlorosis inducing CMV strain with CaCV and GBNV on hot pepper in India.

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Section: Methodsmentioning

confidence: 99%

Co-existence of chlorosis inducing strain of Cucumber mosaic virus with tospoviruses on hot pepper (Capsicum annuum) in India

Vinodhini

Rajendran

Abirami

et al. 2021

Sci Rep

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“…Similarly Nagendran et al (2018) reported the presence of CMV in the virus infected samples of cucurbitaceous crops viz., bottle gourd and snake gourd in areas of Tamil Nadu and found that the virus isolates were found in close resemblance with subgroup of IB. Pratap et al (2008) categorized the subgroup of CMV strains based on the coat protein sequence analysis infecting tomato in India.…”

Section: Characteristic Symptoms Ofmentioning

confidence: 96%

“…Subgroup I was divided into IA and IB based on phylogenetic analysis of coat protein gene. CMV subgroups IA and II are present worldwide, while subgroup IB is originated from Asia (Nagendran et al, 2018). The molecular analysis and determination of the phy logenetic status of viruses is one of the most important steps towards the elucidation of the virus epidemiology and is necessary for the control of plant viral diseases, by introducing appropriate varieties for cultivation by farm ers.…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection and Phylogenetic Analysis of Cucumber Mosaic Virus Infecting Bhut Jolokia (Capsicum chinense Jacq.) of Assam

Borah¹,

Kumar²,

Siddappa³

2019

IJEP

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Prevalence of Cucumber mosaic virus (CMV) in Bhut Jolokia (Capsicum chinense Jacq.) in Assam was confirmed by reverse transcriptionpolymerase chain reaction (RT-PCR). The symptomatic expression of CMV infection onto Bhut Jolokia was confirmed as chlorotic yellow mosaic appearance of the leaves, smaller leaves, reduction of plant growth, mosaic and mottling of infected leaves, leaves showing shoestring or filliform symptom and leaf puckering based on RT-PCR. This is the first report on molecular characterization of CMV in Assam, India. The RT-PCR diagnosis revealed presence of CMV with a band length of 593bp which has been obtained from CMV infected samples. The amplified coat protein gene of CMV was cloned and sequenced. Phylogenetic analysis of coat Protein gene of CMV showed close relatedness with African isolates of sub group IB and other isolates of the world.

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“…CMV has been categorized into subgroup I and II based on serology, host range and peptide mapping. Further, subgroup I is divided into subgroup IA and IB based on 5′ non-coding region of RNA3 and sequence similarity (Roossinck 2001;Revathy and Bhat 2017;Nagendran et al 2018). Subgroup IA and II have worldwide distribution whereas subgroup IB found in Asian countries and comparatively, subgroup I strains are more virulent than subgroup II strains.…”

Section: Introductionmentioning

confidence: 99%

Characterization of cucumber mosaic virus (CMV) subgroup IB infecting chilli in Tamil Nadu, India

et al. 2020

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CMV (cucumber mosaic virus) is the most primitive virus infecting chilli (Capsicum annuum. L). The mosaic incidence with leaf filiformity, mosaic mottling and stunted growth was observed in major chilli growing regions of Tamil Nadu. CMV sap was inoculated on chilli, cowpea, bitter gourd, bottle gourd, ridge gourd, banana, cucumber, Nicotiana and Chenopodium plants. Host range studies revealed that CMV produced localized infection on Nicotiana and systemic symptoms on most of the test plants. The occurrence of CMV was confirmed through DAC-ELISA and RT-PCR analysis. Host plant samples tested with DAC-ELISA showed strong reaction with 1.7 optical density. For molecular characterization, total RNA isolated from infected plants used in RT-PCR with CMV specific primers. The specific amplicons were cloned and sequenced. The complete genome sequencing depicts CMV-RNA1 consist of 3339 nucleotides (nt), RNA2 and RNA3 with 3052nt and 2027nt respectively. Phylogenetic and nucleotide sequence analysis showed TN CMV isolates closely associated with subgroup IB rather than subgroup IA and II. Comparative sequence analysis indicates replicase protein to be more variable among five genes. CP sequence analysis showed 97-98 per cent identity with subgroup IB strains, 92-93 per cent identity with subgroup IA strains and 81-82 per cent identity with subgroup II strains. CMV-RNA3 was predicted to have recombination with Indian black pepper isolate (KU947031) between 165-505nt and Egyptian tomato isolate (KX014666) between 165-506nt positions.

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Characterization of Cucumber mosaic virus infecting snake gourd and bottle gourd in India

Cited by 14 publications

References 16 publications

Co-existence of chlorosis inducing strain of Cucumber mosaic virus with tospoviruses on hot pepper (Capsicum annuum) in India

Co-existence of chlorosis inducing strain of Cucumber mosaic virus with tospoviruses on hot pepper (Capsicum annuum) in India

Molecular Detection and Phylogenetic Analysis of Cucumber Mosaic Virus Infecting Bhut Jolokia (Capsicum chinense Jacq.) of Assam

Characterization of cucumber mosaic virus (CMV) subgroup IB infecting chilli in Tamil Nadu, India

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