Characterization and discrimination of polysaccharides from different species of Cordyceps using saccharide mapping based on PACE and HPTLC

Wu, Ding‐Tao; Cheong, Kit-Leong; Wang, Lanying; Lv, Guangping; Ju, Yaojun; Feng, Ke; Zhao, Jing; Li, Shao‐Ping

doi:10.1016/j.carbpol.2013.12.034

Cited by 63 publications

(32 citation statements)

References 26 publications

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“…Usually, in order to reveal more chemical characters of natural polysaccharides using saccharide mapping based on single enzymatic digestion, more experiments are required (Lin et al, 2015;Wu, Cheong, et al, 2014). Therefore, in order to reduce the experiment times and improve the efficiency of saccharide mapping, composite enzyme (mixture of various enzymes) was used for joint hydrolysis of polysaccharides.…”

Section: Pace and Hptlc Fingerprints Of Composite Enzymatic Digestionmentioning

confidence: 99%

“…Saccharide mapping based on HPTLC and PACE has been proven to be one of powerful methods for the routine analysis of monosaccharides and oligosaccharides derived from polysaccharides in tonic and medicinal fungi Wu, Cheong, et al, 2014). Therefore, partial acid hydrolysates of polysaccharides in L. barbarum collected in different regions of China were compared by using saccharide mapping based on PACE and HPTLC analysis.…”

Section: Pace and Hptlc Fingerprints Of Partial Acid Hydrolysates Of mentioning

confidence: 99%

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Characterization and comparison of polysaccharides from Lycium barbarum in China using saccharide mapping based on PACE and HPTLC

Cheong

Deng

et al. 2015

Carbohydrate Polymers

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Section: Pace and Hptlc Fingerprints Of Composite Enzymatic Digestionmentioning

confidence: 99%

Section: Pace and Hptlc Fingerprints Of Partial Acid Hydrolysates Of mentioning

confidence: 99%

Characterization and comparison of polysaccharides from Lycium barbarum in China using saccharide mapping based on PACE and HPTLC

Cheong

Deng

et al. 2015

Carbohydrate Polymers

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“…Results showed that both PACE and HPTLC fingerprints of pectinase, α-amylase, and β- D -glucanase digested polysaccharides in Bhutanese Cordyceps were similar (Fig. S4B–D), which suggested that α-1,4-galactosidic, α-1,4-glucosidic, and β-1,4-glucosidic linkages might exist in polysaccharides from Bhutanese Cordyceps3435.…”

Section: Resultsmentioning

confidence: 93%

“…S4A). To improve the specificity, pectinase, α-amylase, and β- D -glucanase were selected for enzymatic digestion of polysaccharides from Bhutanese Cordyceps3435. Results showed that both PACE and HPTLC fingerprints of pectinase, α-amylase, and β- D -glucanase digested polysaccharides in Bhutanese Cordyceps were similar (Fig.…”

Section: Resultsmentioning

confidence: 94%

“…Saccharide mapping based on HPTLC and PACE analysis has been proven to be a feasible and desirable technique for qualitative analysis of monosaccharides and oligosaccharides released from polysaccharides, which has been successfully applied for partial characterization and comparison of polysaccharides from natural and cultural C. sinensis and their related species3334. Therefore, partial acid hydrolysates and enzymatic digestions of polysaccharides in Bhutanese Cordyceps were investigated and compared using saccharide mapping based on PACE and HPTLC analysis.…”

Section: Resultsmentioning

confidence: 99%

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Cordyceps collected from Bhutan, an appropriate alternative of Cordyceps sinensis

Zheng

et al. 2016

Sci Rep

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Natural Cordyceps collected in Bhutan has been widely used as natural Cordyceps sinensis, an official species of Cordyceps used as Chinese medicines, around the world in recent years. However, whether Cordyceps from Bhutan could be really used as natural C. sinensis remains unknown. Therefore, DNA sequence, bioactive components including nucleosides and polysaccharides in twelve batches of Cordyceps from Bhutan were firstly investigated, and compared with natural C. sinensis. Results showed that the fungus of Cordyceps from Bhutan was C. sinensis and the host insect belonged to Hepialidae sp. In addition, nucleosides and their bases such as guanine, guanosine, hypoxanthine, uridine, inosine, thymidine, adenine, and adenosine, as well as compositional monosaccharides, partial acid or enzymatic hydrolysates, molecular weights and contents of polysaccharides in Cordyceps from Bhutan were all similar to those of natural C. sinensis. All data suggest that Cordyceps from Bhutan is a rational alternative of natural C. sinensis, which is beneficial for the improvement of their performance in health and medicinal food areas.

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Metabolomic comparison between wild Ophiocordyceps sinensis and artificial cultured Cordyceps militaris

Chen

Guo

Zheng

et al. 2018

Biomedical Chromatography

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A systematic study on the metabolome differences between wild Ophiocordyceps sinensis and artificial cultured Cordyceps militaris was conducted using liquid chromatography-mass spectrometry. Principal component analysis and orthogonal projection on latent structure-discriminant analysis results showed that C. militaris grown on solid rice medium (R-CM) and C. militaris grown on tussah pupa (T-CM) evidently separated and individually separated from wild O. sinensis, indicating metabolome difference among wild O. sinensis, R-CM and T-CM. The metabolome differences between R-CM and T-CM indicated that C. militaris could accommodate to culture medium by differential metabolic regulation. Hierarchical clustering analysis was further performed to cluster the differential metabolites and samples based on their metabolic similarity. The higher content of amino acids (pyroglutamic acid, glutamic acid, histidine, phenylalanine and arginine), unsaturated fatty acid (linolenic acid and linoleic acid), peptides, mannitol, adenosine and succinoadenosine in O. sinensis make it as an excellent choice as a traditional Chinese medicine for invigoration or nutritional supplementation. Similar compositions with O. sinensis and easy cultivation make artificially cultured C. militaris a possible alternative to O. sinensis.

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Characterization and discrimination of polysaccharides from different species of Cordyceps using saccharide mapping based on PACE and HPTLC

Cited by 63 publications

References 26 publications

Characterization and comparison of polysaccharides from Lycium barbarum in China using saccharide mapping based on PACE and HPTLC

Characterization and comparison of polysaccharides from Lycium barbarum in China using saccharide mapping based on PACE and HPTLC

Cordyceps collected from Bhutan, an appropriate alternative of Cordyceps sinensis

Metabolomic comparison between wild Ophiocordyceps sinensis and artificial cultured Cordyceps militaris

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