Characterization and comparison of polysaccharides from Lycium barbarum in China using saccharide mapping based on PACE and HPTLC

Wu, Ding‐Tao; Cheong, Kit-Leong; Deng, Yong; Lin, Peng-Cheng; Wei, Feng; Lv, Xiao-Jie; Long, Zerong; Zhao, Jing; Ma, Shuang‐Cheng; Li, Shaoping

doi:10.1016/j.carbpol.2015.07.052

Cited by 55 publications

(28 citation statements)

References 25 publications

(30 reference statements)

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“…Furthermore, chemometric approach was applied to FT-IR, high performance size-exclusion chromatography (HPSEC), and pre-column derivatization high-performance liquid chromatography (PCD-HPLC), which indicated that polysaccharides of goji from different regions barely differed ( Liu W. et al, 2015 ). These findings are supported by a recent research which shows that the molecular structure of polysaccharides from goji ( L. barbarum only) of different regions is highly similar ( Wu et al, 2015 ). This similarity is also shown by our metabolomic analysis, where L. barbarum samples from different cultivation areas cluster together, but differ from L. chinense .…”

Section: Resultssupporting

confidence: 78%

Quality Variation of Goji (Fruits of Lycium spp.) in China: A Comparative Morphological and Metabolomic Analysis

et al. 2018

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Goji (fruits of Lycium barbarum L. and L. chinense Mill.) has been used in China as food and medicine for millennia, and globally has been consumed increasingly as a healthy food. Ningxia, with a semi-arid climate, always had the reputation of producing best goji quality (daodi area). Recently, the increasing market demand pushed the cultivation into new regions with different climates. We therefore ask: How does goji quality differ among production areas of various climatic regions? Historical records are used to trace the spread of goji production in China over time. Quality measurements of 51 samples were correlated with the four main production areas in China: monsoon (Hebei), semi-arid (Ningxia, Gansu, and Inner Mongolia), plateau (Qinghai) and arid regions (Xinjiang). We include morphological characteristics, sugar and polysaccharide content, antioxidant activity, and metabolomic profiling to compare goji among climatic regions. Goji cultivation probably began in the East (Hebei) of China around 100 CE and later shifted westward to the semi-arid regions. Goji from monsoon, plateau and arid regions differ according to its fruit morphology, whereas semi-arid goji cannot be separated from the other regions. L. chinense fruits, which are exclusively cultivated in Hebei (monsoon), are significantly lighter, smaller and brighter in color, while the heaviest and largest fruits (L. barbarum) stem from the plateau. The metabolomic profiling separates the two species but not the regions of cultivation. Lycium chinense and samples from the semi-arid regions have significantly (p < 0.01) lower sugar contents and L. chinense shows the highest antioxidant activity. Our results do not justify superiority of a specific production area over other areas. Instead it will be essential to distinguish goji from different regions based on the specific morphological and chemical traits with the aim to understand what its intended uses are.

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Section: Resultssupporting

confidence: 78%

Quality Variation of Goji (Fruits of Lycium spp.) in China: A Comparative Morphological and Metabolomic Analysis

et al. 2018

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“…Sevage method and organic reagents were used to remove proteins, pigments and other impurities. The structural composition of a LBP can be studied by SDS-PAGE gel electrophoresis, high perfomance size exclusion chromatography (HPSEC), gas-chromatographic–mass-spectrometry (GC-MS), nucleic magnetic resonance (NMR), and matrix-assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-Tof-MS) [ 18 , 21 , 25 ].…”

Section: Constituentsmentioning

confidence: 99%

Systematic Review of Chemical Constituents in the Genus Lycium (Solanaceae)

et al. 2017

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The Lycium genus is widely used as a traditional Chinese medicine and functional food. Many of the chemical constituents of the genus Lycium were reported previously. In this review, in addition to the polysaccharides, we have enumerated 355 chemical constituents and nutrients, including 22 glycerogalactolipids, 29 phenylpropanoids, 10 coumarins, 13 lignans, 32 flavonoids, 37 amides, 72 alkaloids, four anthraquinones, 32 organic acids, 39 terpenoids, 57 sterols, steroids, and their derivatives, five peptides and three other constituents. This comprehensive study could lay the foundation for further research on the Lycium genus.

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“…Actually, only L. barbarum produced in Ningxia is officially recorded as the authentic “Gouqizi.” Therefore, chemical and bioactive comparison of L. barbarum produced in different regions of China is necessary. Previously, the chemical characters of polysaccharides in L. barbarum from different origins (Ningxia, Qinghai, Xinjiang, Inner Mongolia, Gansu, and Shandong) were demonstrated in the similar patterns (Wu and others ). However, to the best of our knowledge, comparison and characterization of small molecules with antioxidant activity in methanol extracts from L. barbarum collected in different origins of China (Ningxia, Qinghai, Inner Mongolia, Xinjiang, and Gansu) has not been demonstrated.…”

Section: Introductionmentioning

confidence: 89%

Comparison and Characterization of Compounds with Antioxidant Activity in Lycium barbarum Using High‐Performance Thin Layer Chromatography Coupled with DPPH Bioautography and Tandem Mass Spectrometry

Lam

Luo

et al. 2016

Journal of Food Science

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Methanol extracts from 50 batches of Lycium barbarum (L. barbarum, wolfberry) in China were compared and characterized using high-performance thin-layer chromatography coupled with 2,2-diphenyl-1-picrylhydrazyl (DPPH) bioautography (HPTLC-DPPH) and electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-Q-TOF-MS/MS), respectively. Results showed that similar components occupying the major antioxidant activity existed in L. barbarum collected from different origins. However, the average antioxidant capacities of methanol extracts of L. barbarum collected in Ningxia were significantly higher than those of Qinghai, Xinjiang, Inner Mongolia, and Gansu, which may contribute to rational use of L. barbarum in China. Furthermore, the chemical structure of compound with the highest antioxidant capacity was tentatively identified as 2-O-β-d-glucopyranosyl-l-ascorbic acid using ESI-Q-TOF-MS/MS analysis, which possessed high potentials to be used as an antioxidant biomarker for the quality control of L. barbarum. Results are helpful for the bioactivity-based quality control of L. barbarum, and beneficial for the improvement of their performance in functional/health foods area, suggesting that HPTLC-DPPH bioautography with ESI-Q-TOF-MS/MS could be used as a routine approach for quality control of antioxidant components in L. barbarum.

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Characterization and comparison of polysaccharides from Lycium barbarum in China using saccharide mapping based on PACE and HPTLC

Cited by 55 publications

References 25 publications

Quality Variation of Goji (Fruits of Lycium spp.) in China: A Comparative Morphological and Metabolomic Analysis

Quality Variation of Goji (Fruits of Lycium spp.) in China: A Comparative Morphological and Metabolomic Analysis

Systematic Review of Chemical Constituents in the Genus Lycium (Solanaceae)

Comparison and Characterization of Compounds with Antioxidant Activity in Lycium barbarum Using High‐Performance Thin Layer Chromatography Coupled with DPPH Bioautography and Tandem Mass Spectrometry

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