Characteristics of the post-thawed Balinese bull semen extended in three different extenders and equilibration times

Amal, Andi Syaiful; Arifiantini, Raden Iis; Setiadi, Mohamad Agus; Said, Syahruddin

doi:10.14710/jitaa.44.2.135-145

Cited by 12 publications

(18 citation statements)

References 32 publications

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“…A completely randomized design with two-factor arrangement (3 breed × 2 age), with 5 repetitions was used. Results are presented as least square means ± standard error of means (LS means ± SEM) (Amal et al 2019). Effects of breed and age were evaluated by ANOVA, while Duncan's test was used to determine differences among the combination of age and breed, using SAS® program version 9.4.…”

Section: Discussionmentioning

confidence: 99%

Breed and age effects on concentration of adiponectin and reproductive performance in Anglo Nubian, Etawah grade and its crossbred bucks

et al. 2021

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Abstract. Hafizuddin, Karja NWK, Praharani L, Setiadi MA. 2021. Breed and age effects on concentration of adiponectin and reproductive performance in Anglo Nubian, Etawah grade and its crossbred bucks. Biodiversitas 22: 1112-1119. The purpose of this study was to evaluate the effect of differences in breed and age on reproductive performance and adiponectin and testosterone production in Anglo Nubian, Etawah grade, and crossbred (Anpera) bucks. A total of 12 bucks with four individuals of each breed were used. This study collected five data points from each buck regarding adiponectin, testosterone, and reproductive performance (libido and semen characteristics). Data were analyzed with factorial analysis of variance and Duncan's test. The result shows that adiponectin concentration between breeds was significantly different (P <0.01). There were also significant differences (P<0.05) in adiponectin concentrations based on buck age. There were also significant interactions with adiponectin concentrations (P <0.01). Furthermore, testosterone concentrations showed significant differences based on breed (P <0.05) and age (P <0.05). There were also significant age-breed interactions affecting testosterone concentrations (P <0.01). Libido and semen characteristics had no significant differences based on breed and age group, and no significant age-breed interactions (P> 0.05). The heterosis effect on adiponectin concentration (48.05%), testosterone concentration (27.68%), libido (-0.61%), semen volume (-1.93%), sperm motility (0.49%), sperm normal morphology (0.18%), and sperm concentration (0.00%) was measured. In conclusion, there is a significant effect of breed, age, and age-breed interactions on the concentration of adiponectin and testosterone in bucks, and both of these variables have a high heterosis effect on crossbred bucks.

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Section: Discussionmentioning

confidence: 99%

Breed and age effects on concentration of adiponectin and reproductive performance in Anglo Nubian, Etawah grade and its crossbred bucks

et al. 2021

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“…The cryoprotectants should ideally have a low molecular weight. Glycerol is an intracellular cryoprotectant that can penetrate the cell, thereby preventing the formation of ice crystals that could otherwise result in membrane rupture [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Green tea extract increases the quality and reduced DNA mutation of post-thawed Kacang buck sperm

Mustofa

Susilowati

Wurlina

et al. 2021

Heliyon

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The study aimed to determine the addition of green tea extract (GTE) in extender on the quality and DNA mutation of post-thawed Kacang buck sperm. The sperm DNA mutation was observed on nicotinamide adenine dinucleotide hydride (NADH) dehydrogenase 1 (ND1) of mitochondrial Deoxyribonucleic Acid (mtDNA). A pool of 12 Kacang buck ejaculates was diluted in skim milk-egg yolk extender contained 0, 0.05, 0.10, and 0.15 mg of GTE/100 mL for T0, T1, T2, and T3 group, respectively. Each of the aliquot groups was packaged in 0.25 mL French mini straw contained 60 million alive sperm and froze according to the protocol. The ND1 mtDNA amplification of samples was carried out Polymerase Chain Reaction machine, followed by DNA sequencing using the Sanger method. Meanwhile, the phylogenetic tree was constructed using the neighbor-joining (NJ) method with MEGA 7.0 software. The results showed that the T2 group maintained the highest quality for Kacang buck post-thawed semen. There was the highest percentages of sperms viability, motility, intact plasma membrane (IPM), the lowest of malondialdehyde (MDA) concentration, sperm DNA fragmentation (SDF), the total and types of ND1 mtDNA mutation frequency. The phylogenetic tree analysis revealed that the clade of the T2 group was most closely related to the sequence reference. However, there was no correlation between the semen quality parameters (sperm viability, motility, IPM, MDA concentration, and SDF) with ND1 mtDNA mutation of post-thawed Kacang buck semen. It could be concluded that GTE was useful as an antioxidant for Kacang buck semen extender for frozen sperm.

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“…Motility and velocity of spermatozoa are (Amal et al, 2019). Another factor that decreases velocity during storage was the production of ROS by damaging the membrane integrity of spermatozoa.…”

Section: Velocity (Vcl Vsl Vap)mentioning

confidence: 99%

“…The ALH values indicate spermatozoa head deviations while swimming (Kathiravan et al, 2011). The increasing ALH value indicates an inferior quality that can disturb the spermatozoa progression movement (Amal et al, 2019). The decreasing ALH value indicates that the spermatozoa are moving straighter and the speed is slower.…”

Section: Linearity (Lin) Straightness (Str) and Wobble (Wob)mentioning

confidence: 99%

Motility characterization of albumin sexed spermatozoa in two different diluents and additional antioxidant

Ratnawati

Luthfi

Pamungkas

et al. 2020

J. Indonesian Trop. Anim. Agric.

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The purpose of this study was to characterize spermatozoa motility of sexed semen (X and Y) using albumin by diluents and antioxidant treatments. The material used in this study was fresh semen of Ongole Crossbreed (OC) bull with progressive motility >70%. The sperm sexing methodology used albumin gradient of 5%, 10%, and 15%. The diluents used were CEP-2 and andromed with or without the addition of 1mM Glutathione as an antioxidant. The sexed semen was made into liquid semen and stored at 3-5°C. The motility was observed at day 0 (H0) and day 5 (H5). Motility was analyzed using SCA v.2.1. The parameters measured were total motility, progressive motility, VCL, VSL, VAP, LIN, STR, WOB, ALH, BCF and sperm hyperactive. The experimental applied was the factorial pattern 2 x 2. The first factor was the type of diluents (CEP-2 and Andromed), and the second factor was additional glutathione (with or without). Data was analyzed by general linear model of SPSS-IBM 24 program. Sexed semen with CEP-2 diluents showed better motility of spermatozoa than andromed in the upper layers (spermatozoa X) and lower layers (spermatozoa Y). The CEP-2 diluent had a big role to maintain motility, progressive motility and velocity spermatozoa during cold storage. The addition of Glutathione 1mM could support the motility of sexed spermatozoa at cold storage, especially for LIN, STR, and WOB values.

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Characteristics of the post-thawed Balinese bull semen extended in three different extenders and equilibration times

Cited by 12 publications

References 32 publications

Breed and age effects on concentration of adiponectin and reproductive performance in Anglo Nubian, Etawah grade and its crossbred bucks

Breed and age effects on concentration of adiponectin and reproductive performance in Anglo Nubian, Etawah grade and its crossbred bucks

Green tea extract increases the quality and reduced DNA mutation of post-thawed Kacang buck sperm

Motility characterization of albumin sexed spermatozoa in two different diluents and additional antioxidant

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