ABSTRAKTujuan dari penelitian ini adalah untuk menguji efektivitas dari tiga macam bahan pengencer (Tris kuning telur, Bioxcell® dan Optixcell®) dan tiga waktu ekuilibrasi (T-30 min, T-2 jam dan T-4 jam) untuk kriopreservasi semen sapi bali. Tiga puluh enam ejakulat semen berasal dari 6 ekor sapi bali jantan dikoleksi dan dibekukan mengikuti protokol pembekuan rutin. Evaluasi motilitas sperma postthawing (PTM) dilakukan dengan computer assisted semen analysis (CASA). Integritas membran plasma dievaluasi dengan hypo-osmotic swelling (HOS) test dan viabilitas dievaluasi dengan pewarnaan eosin nigrosin. Hasil penelitian menunjukkan terdapat interaksi yang signifikan antara waktu ekuilibrasi dan pengencer terhadap motilitas, viabilitas dan integritas membran sperma sapi bali. Perlakuan kontrol (4 jam waktu ekuilibrasi) memiliki nilai tertinggi (P<0,05) dan perlakuan 30 menit waktu ekuilibrasi memiliki nilai terrendah (P<0,05) untuk total motilitas, motilitas progresif, persentase sperma dengan membran plasma utuh (MPU) dan viabilitas sperma. Motilitas, skor pergerakan individu dan integritas membran sperma dalam pengencer Optixcell® lebih tinggi dibandingkan dengan semen yang dibekukan dalam pengencer Bioxcell® dan Tris kuning telur. Motilitas total, progresif dan integritas membran sperma terbaik secara keseluruhan terdapat dalam pengencer Tris kuning telur dan Bioxcell® dengan waktu ekuilibrasi 4 jam dan Optixcell® dengan waktu ekuilibrasi 2 dan 4 jam. Kesimpulan berdasarkan analisis subyektif dan obyektif, equilibrasi 30 menit tidak cukup untuk mempertahankan motilitas dan integritas membran sperma yang lebih baik; equilibrasi untuk 2 jam menghasilkan motilitas sperma tertinggi untuk pengencer Optixcell®; dan equilibrasi untuk 4 jam menghasilkan viabilitas tertinggi untuk semua pengencer yang digunakan.Kata kunci: sapi bali, kriopreservasi semen, pengencer semen, waktu ekuilibrasi, CASA. ABSTRACTThe objectives of the present study were to compare and determine the best post-thawed characteristics of balinese bull sperm cryopreserved in three different extenders; animal based (Trisclarified egg yolk (Tris-cEY)), and non-animal based extenders (Bioxcell® (lecithin based) andCharacteristics of Post-thawed Bali Bull Semen (A.S. Amal et al.) 135 Optixcell® (liposome based)) in combination with three different equilibration times (30 minutes, 2 hours, 4hours). Thirty six ejaculates were collected from six Balinese bulls and frozen in three extenders (Tris-cEY, Bioxcell® and Optixcell®) after equilibration in three different times (30 minutes, 2hours and 4hours). Computer-assisted sperm analysis (CASA), hypo-osmotic swelling test (HOST) and eosin nigrosin staining were used in the post-thawed semen analysis. There was a significant interaction between equilibration time and extender type for sperm motility, viability and membrane integrity. Thirty minutes equilibration time had the lowest values (P<0.05) for all the evaluated parameters independent of extender type. Overall, semen extended in Tris-cEY, Bioxcell® and O...

Gondanglegi Kulon Village is located in Gondanglegi District, Malang Regency, the research location is in the Muhammadiyah Entrepreneur Islamic Boarding School area. From the results of information from local villagers, it was explained that in the study area there was no shallow groundwater found. This is also reinforced by the fact that there are no wells in the settlements of the residents around the Entrepreneur Muhammadiyah Islamic Boarding School, so that practically no residents use wells to fulfill clean water, they still depend on river water or PDAM services which are relatively expensive and continuous. still below expectations. As a center for religious education, Islamic boarding schools continue to develop supporting facilities. One of the most needed facilities is the availability of clean water. Quantitatively, the supply from the PDAM is not sufficient and the operational costs borne by the manager are too high. The use of groundwater is a solution that can be used to meet the needs of clean water at the research location. Therefore we need a technology to identify the point of exploration, the technology used in this research is geoelectric resistivity. By using the resistivity geoelectric method, it is expected to know the rock structure at the research location and see the potential for deep groundwater. Thus, the hope that in the future the Entrepreneur Muhammadiyah Islamic Boarding School (PPEM) will no longer depend on water raw materials from the PDAM, so the hope is that daily operational costs related to clean water needs can be minimized. From the results of the analysis and discussion, 2 (two) points of drilling recommendations were obtained, but with technical and non-technical considerations, exploration was suggested at point 3 with a potential of 4.2423 L / sec. Where at this location is a rock lithological arrangement consisting of a top soil layer, a clay layer, a tuff layer, a sandy silt layer, a sand layer and a gravel layer.

Road is the prime medium of land transportation that consists of some material like hasrh agregate, smooth agregate, filler, bitumen and also additive materials with certain percentage. Latex is one of additive material that able to be used. The aim of this research is to know about the influence of Latex additition to nature of mixture at Asphalt Treated Base. The mixture planning (mix job) which is gotten from this research is harsh agregate equal to 47,10%, sand equal to 24,03%, stone ash equal to 24,03% (comparison 1 : 1) and filler 4,84%. From this mix job, it is made 60 object test that has rate variation of asphalt 0% - 6% without using Latex. The form of these objects is cylinder. Their measurement is diameter 10 cm, height 7,5 cm and volume 1200 gram. The result of Latex and asphalt mixing shows that the influence of Latex is very real to the stability parameter, irrigate void, flow and Marshall Quotient Optimum Rate Latex is equal to 2,5% with optimal asphalt rate is equal to 3,7%.Keywords : asphalt AC 60/70, Latex

Passenger public transportation in Tuban is one of the means of transport used by the community to meet the transport needs. Total fleet of public transport passengers registered and active operating as many as 40 vehicles, consisting of 30 units of passenger public transportation route A and 10 units of passenger public transportation route B, with a capacity of 12 passengers/vehicle. Passenger public transportation operates from 06.00 - 17.00 with the number of trips ranging from 7-8 trips/day every vehicle and can carry 9-10 passengers/trip every vehicle. The waiting time of passenger route A average of 3 minutes, and route B average of 9 minutes. The purpose of research is to determine the performance and productivity of the passenger public transportation of Tuban. The research using empirical methods to obtain information from observations in the field and these observations are presented in the form of data.The research results it is known that the performance and productivity of public transportation on route A and B. Route A has an average load factor of 81,39%, an average frequency of 20,97 vehicles/hour, an average headway of 2.88 minutes, an average travel time of 3,50 minutes/km, an average travel speed of 21,52 km/hour, and a productivity 7,62 trips/day-vehicle, with 74,45 passengers/day-vehicle. Route B has an average load factor of 78,19%, an average frequency of 7,15 vehicles/hour, an average headway of 8,93 minutes, an average travel time of 2,39 minutes/km, an average travel speed of 21,78 km/hour, and a productivity 7,74 trips/day-vehicle, with 72,6 passengers/day-vehicle..

Jembatan adalah suatu konstruksi yang gunanya untuk meneruskan jalan melalui rintangan yang berada lebih rendah. Rintangan ini biasanya jalan lain berupa jalan air atau jalan lau lintas biasa. Sedangkan desain jembatan bervariasi tergantung pada fungsi dari jembatan, atau kondisi bentuk permukaan bumi dimana jembatan ini di bangun.Bila ditinjau kerusakan jembatan dalam hal ini jembatan jalan raya dimana struktur jembatan masih baik / layak dipergunakan namun lantai jembatan yang mengalami kerusakan. Untuk mengatasi kerusakan lantai jembatan atau pergantian lantai jembatan perlu metode yang khusus untuk mendapatkan nilai ekonomis nya dan kendaraan lalu lintas tetap beroperasi. Salah satu metode untuk penggantian lantai jembatan adalah dengan menggunakan double tee. Keuntungan Memakai Lantai Double Tee antara lain adalah, Pelaksanaannya lebih mudah, waktu pelaksanaan lebih singkat dan biaya lebih murah.Seperti halnya jembatan Ngantru kabupaten Ngawi yang secara struktur jembatan masih layak/baik untuk di pergunakan, namun lantai jembatan mengalami kerusakan.