Characteristics of Lingonberry Plants Propagated by in Vitro and Ex Vitro Propagation Methods

In vitro REGENERATION OF Fragraria PLANTS

“…Moreover, they are characterized by a greater vigour [Karhu 2001, Karhu andHakala 2002]. Besides of that, plants cultivated both on solid and liquid media are characterized by a higher content of anthocyanins and antioxidants than those grown with conventional methods [Debnath 2009]. Different results has been obtained by Mohamed et al [2007], who reported that, more efficient regeneration from lamina was obtained from explants taken from two months old plants growing in a greenhouse than from explants taken from plants growing earlier in vitro.…”

“…One of them is washing in tap water for 30 min initially and next, sterilization in 10% (v/v) Domestos solution for 20 min and after that rinsing six times in sterile distilled water [Passey et al 2003]. Domestos formulation may be replaced by 5,25% solution of sodium hypochlorite (NaOCl) [Kumar et al 1999] or 12% solution of a mixed solutions (vol/vol) of 0,63% NaOCl and 0,1% (v/v) Tween 20 formulation -sterilization for 15 min and next, rinse in 70% ethanol solution for 30 second [Debnath 2009]. Tween 20 formulation was also used by other authors [Yildirim and Turker 2014].…”

“…The most commonly used and most efficient growth regulators used in multiplication and regeneration are TDZ and 2,4-D. TDZ was used in shoot regeneration of Fragaria plants by many authors [Passey et al 2003, Zhao et al 2004, Husaini and Srivastava 2006, Husaini 2010, similarly as BAP [Foucault and Letouze 1987, Sorvari et al 1993, Żebrowska and Hortyński 2002, Lal et al 2003, Kaushal et al 2004. One of disadvantages of TDZ is possibility of inhibitory effect occurring on shoot elongation [Debnath 2009] which is associated with their cytokinin activity [Huetteman and Preece 1993] which occured also in strawberries [Jones et al 2003]. Consequently, a combination of TDZ and IBA (indole-3-butyric acid) activity induced the higher regeneration frequency on the same genotypes, which confirms positive effects of this auxin in strawberry leaf tissue callogenesis [Yonghua et al 2004, Husaini and Srivastava 2006, Landi and Mezzetti 2006, Yildirim and Turker 2014.…”

“…Fragaria plants (Rosaceae family) are a perennial plants, which taste attributes of fruits are considered very attractive for a huge number of consumers around the world [Debnath 2009]. There are more than 12 species in Fragaria genus.…”

“…Content of phenols (especially ellagic acid) in strawberries causes their health-promoting qualities [Skupień and Oszmiański 2004]. They have also antioxidative properties [Debnath 2009]. The strawberry fruits contain vitamin C in an amount exceeding oranges and lemons [Bhatt and Dhar 2000].…”